Genetic differentiation of <i>Echinostoma revolutum</i> and <i>Hypodereaum conoideum</i> from domestic ducks in Thailand by multilocus enzyme electrophoresis

Saijuntha, Weerachai; Sithithaworn, Paiboon; Andrews, Ross H.

doi:10.1017/s0022149x09990393

Cited by 13 publications

(3 citation statements)

References 20 publications

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“…Members of these genera are quite common in the environment and use common snail species as first or second intermediate hosts (Cichy and Żbikowska, 2016). Echinostomes are widespread flukes causing intestinal diseases and they are mainly important in wildlife diversity (Saijuntha et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Potamopyrgus antipodarum (Gray, 1843) in Polish waters − its mitochondrial haplotype and role as intermediate host for trematodes

Stanicka

Zając

Lachowska‐Cierlik

et al. 2020

Knowl. Manag. Aquat. Ecosyst.

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The New Zealand mud snail (Potamopyrgus antipodarum (Gray, 1843)) is on the list of one hundred worst invasive species. Researchers point out that genetic variation between populations of P. antipodarum manifested in differences in life-history traits. The main objective of our investigation was to gain pioneer knowledge about mitochondrial haplotypes of P. antipodarum in Polish waters on the background of these haplotypes recorded in the world and confirmation of the main role of P. antipodarum in the life cycle of digenean trematodes. We examined 1000 individuals of P. antipodarum from five water bodies in three different parts of Poland for the presence of larval stages of digenean trematodes. For several randomly selected individuals we carried out DNA sequencing of the 16S ribosomal RNA gene as marker of this non-indigenous mollusk. Only one 16S rRNA haplotype of P. antipodarum was recorded in Polish waters, defined in this study as haplotype 1 which turned out to be the most widespread in Europe. Potamopyrgus antipodarum is a source of trematode metacercariae belonging mainly to the family Echinostomatidae. As a result, we can demonstrate that it plays a role as the second intermediate host of digenean trematodes in European waters.

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Section: Discussionmentioning

confidence: 99%

Potamopyrgus antipodarum (Gray, 1843) in Polish waters − its mitochondrial haplotype and role as intermediate host for trematodes

Stanicka

Zając

Lachowska‐Cierlik

et al. 2020

Knowl. Manag. Aquat. Ecosyst.

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“…The nad 1 sequence variation among the “ E. revolutum group” indicated that E. revolutum haplotypes from Europe formed a monophyletic group which clustered closely with a monophyletic group of isolates from North America [ 45 ]. In Asia, molecular genetic studies were conducted on E. revolutum in comparison with Hypoderaeum conoideum , Echinoparyphium recurvatum , and Artyfechinostomum malayanum (under the name Echinostoma malayanum ) using multilocus enzyme electrophoresis and sequencing of ITS1 and cox 1 [ 52 , 53 , 109 , 110 ]. However, isolates from Southeast Asia were not included in the study of Georgieva et al [ 45 ] due to the lack of nad 1 data [ 72 ].…”

Section: Species Of 37-collar-spined Echinostoma Gmentioning

confidence: 99%

Taxonomy of Echinostoma revolutum and 37-Collar-Spined Echinostoma spp.: A Historical Review

et al. 2020

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Echinostoma flukes armed with 37 collar spines on their head collar are called as 37-collar-spined Echinostoma spp. (group) or ‘Echinostoma revolutum group’. At least 56 nominal species have been described in this group. However, many of them were morphologically close to and difficult to distinguish from the other, thus synonymized with the others. However, some of the synonymies were disagreed by other researchers, and taxonomic debates have been continued. Fortunately, recent development of molecular techniques, in particular, sequencing of the mitochondrial (nad1 and cox1) and nuclear genes (ITS region; ITS1-5.8S-ITS2), has enabled us to obtain highly useful data on phylogenetic relationships of these 37-collar-spined Echinostoma spp. Thus, 16 different species are currently acknowledged to be valid worldwide, which include E. revolutum, E. bolschewense, E. caproni, E. cinetorchis, E. deserticum, E. lindoense, E. luisreyi, E. mekongi, E. miyagawai, E. nasincovae, E. novaezealandense, E. paraensei, E. paraulum, E. robustum, E. trivolvis, and Echinostoma sp. IG of Georgieva et al., 2013. The validity of the other 10 species is retained until further evaluation, including molecular analyses; E. acuticauda, E. barbosai, E. chloephagae, E. echinatum, E. jurini, E. nudicaudatum, E. parvocirrus, E. pinnicaudatum, E. ralli, and E. rodriguesi. In this review, the history of discovery and taxonomic debates on these 26 valid or validity-retained species are briefly reviewed.

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“…As a consequence, a variety of genetic markers have been developed and used effectively to differentiate among echinostome species and explore their population genetic and phylogenetic relationships between/among different genera, species, and geographic areas, as well as temporally and in relation to intermediate hosts (Kostadinova et al 2003, Detwiler et al 2010, Saijuntha et al 2010a. Genetic variation in echinostomes in Southeast Asia has been studied using allozyme markers and the cytochrome c oxidase subunit 1 (CO1) sequence (Saijuntha et al 2010a, b).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of Echinostoma revolutum and Echinoparyphium recurvatum (Trematoda: Echinostomatidae) in Thailand and phylogenetic relationships with other isolates inferred by ITS1 sequence

et al. 2010

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Echinostomatidae are common, widely distributed intestinal parasites causing significant disease in both animals and humans worldwide. In spite of their importance, the taxonomy of these echinostomes is still controversial. The taxonomic status of two species, Echinostoma revolutum and Echinoparyphium recurvatum, which commonly infect poultry and other birds, as well as human, is problematical. Previous phylogenetic analyses of Southeast Asian strains indicate that these species cluster as sister taxa. In the present study, the first internal transcribed spacer (ITS1) sequence was used for genetic characterization and to examine the phylogenetic relationships between an isolate from Thailand with other isolates available from GenBank database. Interspecies differences in ITS1 sequence between E. revolutum and E. recurvatum were detected at 6 (3%) of the 203 alignment positions. Of these, nucleotide deletion at positions 25, 26, and 27, pyrimidine transition at 50, 189, and pyrimidine transversion at 118 were observed. Phylogenetic analysis revealed that E. recurvatum from Thailand clustered as a sister taxa with E. revolutum and not with other members of the genus Echinoparyphium. Interestingly, this result confirms a previous report based on allozyme electrophoresis and mitochondrial DNA that E. revolutum and E. recurvatum in Southeast Asia are sister species. Hence, the taxonomic status of E. recurvatum in Thailand, as well as in Southeast Asian countries needs to be confirmed and revised using more comprehensive analyses based on morphology and other molecular techniques.

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Genetic differentiation of Echinostoma revolutum and Hypodereaum conoideum from domestic ducks in Thailand by multilocus enzyme electrophoresis

Cited by 13 publications

References 20 publications

Potamopyrgus antipodarum (Gray, 1843) in Polish waters − its mitochondrial haplotype and role as intermediate host for trematodes

Potamopyrgus antipodarum (Gray, 1843) in Polish waters − its mitochondrial haplotype and role as intermediate host for trematodes

Taxonomy of Echinostoma revolutum and 37-Collar-Spined Echinostoma spp.: A Historical Review

Genetic characterization of Echinostoma revolutum and Echinoparyphium recurvatum (Trematoda: Echinostomatidae) in Thailand and phylogenetic relationships with other isolates inferred by ITS1 sequence

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