Genetic Dissection of Fe-Dependent Signaling in Root Developmental Responses to Phosphate Deficiency

Wang, Xiaoyue; Wang, Zhen; Zheng, Zai; Dong, Jinsong; Song, Li; Sui, Liqian; Nussaume, Laurent; Desnos, Thierry; Liu, Dong

doi:10.1104/pp.18.00907

Cited by 76 publications

(75 citation statements)

References 42 publications

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“…Using the pSTOP1::GFP‐STOP1 reporter we previously showed that STOP1 accumulates in the nuclei at the primary root tips of seedlings grown in –Pi (Balzergue et al ., ; Wang et al ., ). Our gene expression analysis of ALMT1 led us to test whether Fe is involved in this nuclear accumulation of STOP1.…”

Section: Resultsmentioning

confidence: 97%

“…Using the pSTOP1::GFP‐STOP1 marker we have previously shown that in the als3 and star1 mutants, the accumulation of GFP‐STOP1 in root nuclei is much higher than in the WT (Wang et al ., ). We thus asked whether this overaccumulation depends on Fe.…”

Section: Resultsmentioning

confidence: 97%

“…We, and others, have previously shown that several aspects of the root growth arrest under –Pi conditions are dependent on Fe (Svistoonoff et al ., ; Ward et al ., ; Muller et al ., ; Dong et al ., ; Gutiérrez‐Alanís et al ., ; Singh et al ., ; Wang et al ., ), but the exact relationships between –Pi and Fe were unclear. Here, we focused our work on STOP1 signaling and succeeded in dissociating the effect of –Pi itself from the role of Fe in STOP1 signaling.…”

Section: Discussionmentioning

confidence: 99%

“…By contrast, in –Pi, overexpression of the ALS3–STAR1 fusion protein represses the accumulation of STOP1 in the nucleus and improves root growth of the WT. Moreover, a suppressor screen of als3 identified stop1 and almt1 mutants (as well as lpr1 ) (Wang et al ., ). Taken together, these results show that, in combination, ALS3 and STAR1 attenuate root growth inhibition in –Pi by repressing the accumulation of STOP1 in the nucleus.…”

Section: Introductionmentioning

confidence: 97%

“…Taken together, these results show that, in combination, ALS3 and STAR1 attenuate root growth inhibition in –Pi by repressing the accumulation of STOP1 in the nucleus. This led us to hypothesize that ALS3–STAR1 depletes an unknown cytosolic compound (toward the vacuole) that enhances the accumulation of STOP1 in the nucleus (Wang et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Under phosphate starvation conditions, Fe and Al trigger accumulation of the transcription factor STOP1 in the nucleus of Arabidopsis root cells

et al. 2019

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Summary Low‐phosphate (Pi) conditions are known to repress primary root growth of Arabidopsis at low pH and in an Fe‐dependent manner. This growth arrest requires accumulation of the transcription factor STOP1 in the nucleus, where it activates the transcription of the malate transporter gene ALMT1; exuded malate is suspected to interact with extracellular Fe to inhibit root growth. In addition, ALS3 – an ABC‐like transporter identified for its role in tolerance to toxic Al – represses nuclear accumulation of STOP1 and the expression of ALMT1. Until now it was unclear whether Pi deficiency itself or Fe activates the accumulation of STOP1 in the nucleus. Here, by using different growth media to dissociate the effects of Fe from Pi deficiency itself, we demonstrate that Fe is sufficient to trigger the accumulation of STOP1 in the nucleus, which, in turn, activates the expression of ALMT1. We also show that a low pH is necessary to stimulate the Fe‐dependent accumulation of nuclear STOP1. Furthermore, pharmacological experiments indicate that Fe inhibits proteasomal degradation of STOP1. We also show that Al acts like Fe for nuclear accumulation of STOP1 and ALMT1 expression, and that the overaccumulation of STOP1 in the nucleus of the als3 mutant grown in low‐Pi conditions could be abolished by Fe deficiency. Altogether, our results indicate that, under low‐Pi conditions, Fe2/3+ and Al3+ act similarly to increase the stability of STOP1 and its accumulation in the nucleus where it activates the expression of ALMT1.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

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Under phosphate starvation conditions, Fe and Al trigger accumulation of the transcription factor STOP1 in the nucleus of Arabidopsis root cells

et al. 2019

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Prospects of genetics and breeding for low-phosphate tolerance: an integrated approach from soil to cell

et al. 2022

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Improving phosphorus (P) crop nutrition has emerged as a key factor toward achieving a more resilient and sustainable agriculture. P is an essential nutrient for plant development and reproduction, and phosphate (Pi)-based fertilizers represent one of the pillars that sustain food production systems. To meet the global food demand, the challenge for modern agriculture is to increase food production and improve food quality in a sustainable way by significantly optimizing Pi fertilizer use efficiency. The development of genetically improved crops with higher Pi uptake and Pi-use efficiency and higher adaptability to environments with low-Pi availability will play a crucial role toward this end. In this review, we summarize the current understanding of Pi nutrition and the regulation of Pi-starvation responses in plants, and provide new perspectives on how to harness the ample repertoire of genetic mechanisms behind these adaptive responses for crop improvement. We discuss on the potential of implementing more integrative, versatile, and effective strategies by incorporating systems biology approaches and tools such as genome editing and synthetic biology. These strategies will be invaluable for producing high-yielding crops that require reduced Pi fertilizer inputs and to develop a more sustainable global agriculture.

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Arabidopsis PDE1 confers phosphate-deficiency tolerance in primary root growth

Wang,

Qian,

et al. 2023

Plant Cell Rep

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Genetic Dissection of Fe-Dependent Signaling in Root Developmental Responses to Phosphate Deficiency

Cited by 76 publications

References 42 publications

Under phosphate starvation conditions, Fe and Al trigger accumulation of the transcription factor STOP1 in the nucleus of Arabidopsis root cells

Under phosphate starvation conditions, Fe and Al trigger accumulation of the transcription factor STOP1 in the nucleus of Arabidopsis root cells

Prospects of genetics and breeding for low-phosphate tolerance: an integrated approach from soil to cell

Arabidopsis PDE1 confers phosphate-deficiency tolerance in primary root growth

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