Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus

Chao, Hongbo; Wang, Hao; Wang, Xiaodong; Guo, Liangxing; Gu, Jiacun; Zhao, Weiguo; Li, Baojun; Chen, Dengyan; Raboanatahiry, Nadia; Li, Maoteng

doi:10.1038/srep46295

Cited by 88 publications

(107 citation statements)

References 72 publications

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“…OC-QTLs and FA-QTLs from nine previously reported populations were aligned into one map for comparison: DY (‘Darmor-bzh’ × ‘Yudal’) [ 37 ], RNSL (‘Rapid’ × ‘NSL96/25’) [ 37 ], Z5 (‘zy036’ × ‘51,070’) [ 39 ], SG (‘Sollux’ × ‘Gaoyou’) [ 40 , 41 ], KN (‘KenC-8’ × ‘N53–2’) [ 42 , 43 ], TN (‘Tapidor’ × ‘Ningyou7’) [ 44 , 45 ], SO (Sansibar × Oase) [ 46 ], PT (Polo × Topas) [ 47 ] and M201 × M202 [ 48 ]. The QTLs were projected onto the physical map of the reference genome “ Darmor-bzh” and the position of related flanking markers were identified by using of E-PCR [ 83 , 84 ].…”

Section: Methodsmentioning

confidence: 99%

Synteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus

et al. 2017

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BackgroundDeciphering the genetic architecture of a species is a good way to understand its evolutionary history, but also to tailor its profile for breeding elite cultivars with desirable traits. Aligning QTLs from diverse population in one map and utilizing it for comparison, but also as a basis for multiple analyses assure a stronger evidence to understand the genetic system related to a given phenotype.ResultsIn this study, 439 genes involved in fatty acid (FA) and triacylglycerol (TAG) biosyntheses were identified in Brassica napus. B. napus genome showed mixed gene loss and insertion compared to B. rapa and B. oleracea, and C genome had more inserted genes. Identified QTLs for oil (OC-QTLs) and fatty acids (FA-QTLs) from nine reported populations were projected on the physical map of the reference genome “Darmor-bzh” to generate a map. Thus, 335 FA-QTLs and OC-QTLs could be highlighted and 82 QTLs were overlapping. Chromosome C3 contained 22 overlapping QTLs with all trait studied except for C18:3. In total, 218 candidate genes which were potentially involved in FA and TAG were identified in 162 QTLs confidence intervals and some of them might affect many traits. Also, 76 among these candidate genes were found inside 57 overlapping QTLs, and candidate genes for oil content were in majority (61/76 genes). Then, sixteen genes were found in overlapping QTLs involving three populations, and the remaining 60 genes were found in overlapping QTLs of two populations. Interaction network and pathway analysis of these candidate genes indicated ten genes that might have strong influence over the other genes that control fatty acids and oil formation.ConclusionThe present results provided new information for genetic basis of FA and TAG formation in B. napus. A map including QTLs from numerous populations was built, which could serve as reference to study the genome profile of B. napus, and new potential genes emerged which might affect seed oil. New useful tracks were showed for the selection of population or/and selection of interesting genes for breeding improvement purpose.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4176-6) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Synteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus

et al. 2017

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“…Almost no heterosis was found for seed oil and protein contents in the test hybrid populations of the inbred lines derived from the six interspecific crosses. These two traits are mainly controlled by additive genes (Shen et al, 2005;Variath et al, 2009; for review, see Rahman et al, 2013;Cheng et al, 2016;Chao et al, 2017) which could be the reason for the lack of significant heterosis for these two seed traits as has been found in other studies as well (Grant and Beversdorf, 1985;Rahman et al, 2016). The occurrence of strong positive correlation between the performance of the inbred lines and test hybrids for seed oil and protein contents and weak correlation of these traits with MPH suggests that these two traits are largely controlled by additive genes.…”

Section: Discussionmentioning

confidence: 90%

Potential of the C Genome of the Different Variants of Brassica oleracea for Heterosis in Spring B. napus Canola

et al. 2020

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The genetic base of Brassica napus canola need to be broadened for exploitation of heterosis at a greater level in the breeding of F 1 hybrid canola cultivars. In this study, we evaluated 228 inbred B. napus canola lines derived from six B. napus × B. oleracea interspecific crosses and following two breeding methods (F 2-and BC 1-derived lines) to understand the effect of the B. oleracea alleles on heterosis for different agronomic and seed quality traits. Test hybrids of the inbreds derived from crosses involving vars. botrytis (cauliflower), alboglabra (Chinese kale) and capitata (cabbage) cv. Badger Shipper, on an average, gave about 10% mid-parent heterosis (MPH), and about 67% of the test hybrids gave higher seed yield than the common B. napus parent indicating that B. oleracea alleles can contribute to heterosis for seed yield in spring B. napus canola hybrids. This was also evident from a positive correlation of the genetic distance of the inbred lines from the common B. napus parent with MPH for seed yield (r = 0.31) as well as with hybrid yield (r = 0.26). Almost no correlation was found between genetic distance and MPH for seed oil and protein content as well as with the performance of the test hybrids for these two traits. The occurrence of positive correlation between seed yield of the inbred lines and test hybrids suggested the importance of the genes exerting additive effect for high seed yield in the hybrids. Very little or almost no heterosis was found for the other agronomic traits as well as for seed oil and protein content. While comparing the two breeding methods, no significant difference was found for seed yield of the test hybrids or the level of MPH; however, the BC 1-derived inbred and test hybrid populations flowered and matured earlier and had longer grain-filling period than the F 2-derived population. Thus, the results suggested that the B. oleracea gene pool can be used in the breeding of spring B. napus canola to improve seed yield in hybrid cultivars.

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“…A high density SNP-based linkage map that included more than 3000 markers with an average genetic distance of 0.96 cM was constructed for KN DH population (Chao et al, 2017 ). The map was used for QTL mapping and epistasis analysis for FAs together with phenotype data in the current study.…”

Section: Methodsmentioning

confidence: 99%

Stable, Environmental Specific and Novel QTL Identification as Well as Genetic Dissection of Fatty Acid Metabolism in Brassica napus

et al. 2018

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Fatty acid (FA) composition is the typical quantitative trait in oil seed crops, of which study is not only closely related to oil content, but is also more critical for the quality improvement of seed oil. The double haploid (DH) population named KN with a high density SNP linkage map was applied for quantitative trait loci (QTL) analysis of FA composition in this study. A total of 406 identified QTL were detected for eight FA components with an average confidence interval (CI) of 2.92 cM, the explained phenotypic variation (PV) value ranged from 1.49 to 45.05%. Totally, 204 consensus and 91 unique QTL were further obtained via meta-analysis method for the purpose of detecting multiple environment expressed and pleiotropic QTL, respectively. Of which, 74 stable expressed and 22 environmental specific QTL were also revealed, respectively. In order to make clear the genetic mechanism of FA metabolism at individual QTL level, conditional QTL analysis was also conducted and more than two thousand conditional QTL which could not be detected under the unconditional mapping were detected, which indicated the complex interrelationship of the QTL controlling FA content in rapeseed. Through comparative genomic analysis and homologous gene annotation, 61 candidates related to acyl lipid metabolism were identified underlying the CI of FA QTL. To further visualize the genetic mechanism of FA metabolism, an intuitive and meticulous network about acyl lipid metabolism was constructed and some closely related candidates were positioned. This study provided a more accurate localization for stable and pleiotropic QTL, and a deeper dissection of the molecular regulatory mechanism of FA metabolism in rapeseed.

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Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus

Cited by 88 publications

References 72 publications

Synteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus

Synteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus

Potential of the C Genome of the Different Variants of Brassica oleracea for Heterosis in Spring B. napus Canola

Stable, Environmental Specific and Novel QTL Identification as Well as Genetic Dissection of Fatty Acid Metabolism in Brassica napus

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