Genetic Dissection of Sympatric Populations of Brown Planthopper,Nilaparvata lugens(Stål), Using DALP-PCR Molecular Markers

Abstract

Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper, Nilaparvata lugens species complex for the analysis of genetic polymorphisms. A total of 11 populations of Nilaparvata spp. were collected from 6 locations from Malaysia. Two sympatric populations of brown planthopper, N. lugens, one from rice and the other from a weed grass (Leersia hexandra), were collected from each of five locations. N. bakeri was used as an out group. Three oligonucleotide primer pairs, DALP231/DALPR′5, DALP234/DALPR′5, and DALP235/DALPR′5 were applied in this study. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on genetic distances for the 11 populations of Nilaparvata spp. revealed that populations belonging to the same species and the same host type clustered together irrespective of their geographical localities of capture. The populations of N. lugens formed into two distinct clusters, one was insects with high esterase activities usually captured from rice and the other was with low esterase activities usually captured from L. hexandra. N. bakeri, an out group, was the most isolated group. Analyses of principal components, molecular variance, and robustness also supported greatly to the findings of cluster analysis.

SSR Markers Revealed Genetic Divergence of Rice Brown Planthopper Populations Maintained on Two Sets of Differential Host Varieties

“…Except for population 42, genomic DNAs and PCR amplicons for the remaining populations have been available from previous studies (Chaerani et al 2021;. The SDS buffer extraction protocol (Latif et al 2012) was used for DNA isolation of five females randomly selected from population 42. A total of 38 SSR primers previously used for genetic analysis of BPH field populations and samples of biotype 1 and 2 cultures (Chaerani et al 2015), were tested for polymorphisms on DNA pools of the six populations consisting of five individuals each.…”

Kemiripan genetik wereng coklat, Nilaparvata lugens Stål. (Homoptera: Delphacidae) populasi Klaten dan Yogyakarta berdasarkan penanda RAPD-PCR

“…Teknik randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) telah digunakan untuk studi genetik populasi serangga (Loxdale & Lushai 1998;Kumar & Gurusubramanian 2011), diantaranya kemiripan genetik biotipe wereng batang coklat di Filipina (Shufran & Whalon 1995) dan di Indonesia (Bahagiawati & Rijzaani 2005), serta populasi wereng batang coklat dari beberapa wilayah di Tamil Nadu, India (Deivendran 2015). Sementara itu, Latif et al (2012) menggunakan teknik direct amplification of length polymorphismspolymerase chain reaction (DALP-PCR) untuk mengidentifikasi populasi wereng batang coklat dengan inang berbeda dengan beberapa lokasi di Malaysia. Meskipun demikian, hasil studi genetik wereng batang coklat dengan teknik RAPD-PCR ini belum sepenuhnya mengungkap aspek genetik biotipenya.…”

Adaptation of the brown planthopper,Nilaparvata lugens(Stål), to resistant rice varieties

“…The results of the study clearly imply that endosymbionts influence the performance of herbivores on plants, to such an extent that they can ultimately determine whether a plant becomes a suitable host for the insect or not. It would be interesting to conduct similar studies on, for example, the two cryptic species that constitute the brown planthopper complex (Latif et al 2008(Latif et al , 2012 : Table 1) Table 1), suggests that virulence adaptations are likely to be similarly diverse and complex.…”