Genetic Distances between Tailfin Anchovy (Coilia nasus) Populations Analyzed by PCR

Abstract

The author established a PCR-based genetic platform to examine the hierarchical polar dendrogram of Euclidean genetic distances of one tailfin anchovy population, especially for Coilia nasus, which was further associated with other fish population, by connecting with specifically designed oligonucleotide primer sets. Five oligonucleotide primers were used to generate a total of 260 and 211 scorable fragments in Coilia populations I and II, respectively. The DNA fragments ranged from greater than (approximately) 100 to more than 2,000 bp. The average bandsharing values (BS) of individuals from the anchovy population I (0.693) displayed higher values than individuals from population II (0.675). The genetic distance between individuals established the existence of a close relationship in group II. Comparatively, individuals of one anchovy population were fairly related to other fish populations, as shown in the polar hierarchical dendrogram of Euclidean genetic distances. The noteworthy genetic distance determined between two Coilia nasus populations demonstrates that this PCR technique can be applied as one of the several devices for individuals and/or population biological DNA researches undertaken for safeguarding species and for production of anchovies in the littoral area of Korea.

PCR Analysis for Genetic Distances of Two Charybdis Crab Populations

“…This analysis presented that great MDs could be achieved among individuals of terrestrial sample assembles. The rates of the pairwise appraisals of balanced GD between the populations of the tailfin anchovy ( Coilia nasus ) from the pooled records for the seven ONT-primers, ranged from 0.051 to 0.435 ( Jo & Yoon, 2021 ). The GD between individuals thus confirmed the reality of a neighboring affiliation in group II between two populations of tailfin anchovy.…”

“…( Ghatak et al, 2007 ), rockfish ( Sebastes spp.) ( Yoon et al, 2007 ), gracilaria ( Gracilaria vermiculophylla ) ( Yoon, 2018 ), river pufferfish ( Takifugu obscurus ) ( Yoon, 2020 ), and tailfin anchovy ( C. nasus ) ( Jo & Yoon, 2021 ). The apparent primer revealed significant MDs in parties and groups, resulting from variations in DNA polymorphisms among parties and groups ( Archak et al, 2003 ; Diaz-Jaimes & Uribe-Alcocer, 2003 ; Wang & Li, 2004 ; Wasko et al, 2004 ; Kim et al, 2006 ; Nagarajan et al, 2006 ; Yoon, 2018 ; Yoon, 2020 ; Jo & Yoon, 2021 ).…”

“…Great point of an important GD between two S. subcrenata groups showed this research process is one of the most appropriate apparatuses for biotechnological DNA studies on entities and units of other life beings ( Koh et al, 1998 ; Dixon et al, 2004 ; Araneda et al, 2005 ; Godhe et al, 2006 ; Kang & Yoon, 2013 ; Yoon, 2018 ). It has been described that the ONT-primer was valuable in the documentation of entities and/or groups, resulting from variations in DNA polymorphisms among entities/populations ( Archak et al, 2003 ; Diaz-Jaimes & Uribe-Alcocer, 2003 ; Yoon & Kim, 2003a ; Wang & Li, 2004 ; Wasko et al, 2004 ; Kim et al, 2006 ; Nagarajan et al, 2006 ; Upadhyay et al, 2006 ; Ghatak et al, 2007 ; Yoon et al, 2007 ; Kang & Yoon, 2013 ; Yoon, 2020 ; Jo & Yoon, 2021 ; Yoon, 2021a ).…”

“…Thus, there is a requirement to understand the genetic characters and more statistics of this crab in order to evaluate exactly the accurately genetic suggestion. Above all, the clustering analysis of the genetic distance (GD) between populations/species/genera of several teleost and crustaceans from the different geographic sites has been accomplished by means of PCR technique is a few quantity ( Diaz-Jaimes & Uribe-Alcocer, 2003 ; Wang & Li, 2004 ; Wasko et al, 2004 ; Nagarajan et al, 2006 ; Upadhyay et al, 2006 ; Ghatak et al, 2007 ; Yoon et al, 2007 ; Kang & Yoon, 2013 ; Yoon, 2020 ; Jo & Yoon, 2021 ).…”

Nutritional Components and Quality Evaluation of Coilia nasus Muscle from Offshore of the Yangtze Estuary

“…However, with the impact of water conservancy proj ects' construction along the river, environmental pollution and overfishing, C. nasus resources in the Yangtze River are on the verge of extinction. Therefore, in recent years, a large number of studies have been carried out on the protection and management of C. nasus resources (Ma et al, 2020;Xue et al, 2020), migratory habits (Wang et al, 2020;Xu et al, 2020) and genetic diversity (Jo et al, 2021;Xuan et al, 2021). But, there are relatively few studies on the offshore C. nasus outside the No fishing line (Fig 1).…”