Genetic Distances of Rainbow Trout and Masu Salmon as Determined by PCR-Based Analysis

Abstract

This study used a PCR-based genetic analysis platform to create a hierarchical polar dendrogram of Euclidean genetic distances for two salmonid species, Oncorhynchus mykiss (rainbow trout, RT) and Oncorhynchus masou (masu salmon, MS). The species were distantly related to other fish species based on PCR results from using the designed oligonucleotide primer series. Five oligonucleotide primers were used to generate 330 and 234 scorable fragments in the RT and MS populations, respectively. The DNA fragments ranged in size from approximately 50 bp to more than 2,000 bp. The bandsharing (BS) results showed that the RT population had a higher average BS value (0.852) than that for the MS population (0.704). The genetic distance between individuals supported the presence of adjacent affiliation in cluster I (RT 01-RT 11). The observation of a significant genetic distance between the two Oncorhynchus species verifies that this PCR-based technique can be a useful approach for individual-and population-based biological DNA investigations. The results of this type of investigation can be useful for species safekeeping and the maintenance of salmonid populations in the mountain streams of Korea.

Genetic Variations within and between Blue Crab (Portunus trituberculatus) Groups

“…Parties in the blue crab group beta (0.601±0.017) had higher BS rates than did parties from the blue crab group alpha (0.563±0.017) ( p <0.05), as established in Table 3 . The reported BS rates between the two crab groups are inconsistent with the previously reported results ( Yoon, 2020 ). To illustrate, the average BS rate illustrated by my study is also lower than the value reported for Chinese (0.788±0.014) Urechis spp.…”

“…Higher FM scales (>2,000 bp) are not observed in the two blue crab groups. These results, in fact, indicate that the genome scales of blue crab are in line with to those of the blue catfish ( Liu et al, 1998 ), yellowfin tuna ( Diaz-Jaimes & Uribe-Alcocer, 2003 ), long-fin carp ( Wang & Li, 2004 ), masu salmon ( Yamazaki et al, 2005 ), crayfish ( Yoon et al, 2006 ), cuttlefish ( Yoon & Kim, 2010 ), and white clam ( Yoon et al, 2012 ), and masu salmon ( Yoon, 2020 ). With respect to various fishes, it has been reported that the number of amplified bands varied from three to eight, with the scale range varying from 500 to 3,530 bp in three Channa punctatus groups ( Nagarajan et al, 2006 ).…”

“…To illustrate, the average BS rate illustrated by my study is also lower than the value reported for Chinese (0.788±0.014) Urechis spp. groups ( Yoon, 2019b ), and masu salmon group (0.704) ( Yoon, 2020 ).…”

Genetic Distances between Tailfin Anchovy (Coilia nasus) Populations Analyzed by PCR

“…They reported that the Padma and the Jamuna populations were separated from each other with the lowest genetic distance (D=0.025). Taken together, the data obtained in the current study establishes the perspective of this research method for determining the diagnostic markers for stock, species, genus and geographic population identification of finfish (Zhou et al, 2000;Mamuris et al, 2005;Yoon, 2018;Yoon, 2020), shellfish (Kim et al, 2004;Kim et al, 2006;Yoon et al, 2012;Song & Yoon, 2013;Oh & Yoon, 2014), in plant (Bommineni et al, 1997), and livestock ( Jeffreys & Morton, 1987;Gwakisa et al, 1994;Ye et al, 1998;Sharma et al, 2004;Islam et al, 2005). The points of considerable genetic distance between two Coilia nasus populations proves that this PCR system is one of the several strategies for individual and/or population genetic DNA studies.…”

“…Therefore, in order to evaluate the exact patent genetic consequence, there is a necessity to understand the genetic traits and composition of this finfish population. PCR-based molecular research methods have been applied to study the genetic characters of various finfish and shellfish (Zhou et al, 2000;Mamuris et al, 2005;Park et al, 2005;Kim et al, 2006;Yoon & Kim 2010;Yoon et al, 2012;Oh & Yoon, 2014;Yoon, 2018;Yoon, 2020). Largely, This PCR method is one of fastest and simplest research methods to identify genetic difference and the polymorphism in various organisms that does not require the prior knowledge of the genomic DNA (Bommineni et al, 1997;Mamuris et al, 2005).…”