Genetic divergence of mykizha (Parasalmo (Oncorhynchus) mykiss) from Kamchatka inferred from restriction analysis and sequencing of mtDNA cytochrome b gene

Павлов, С. Д.; Kolesnikov, Alexander; Melnikova, M. N.; Ushakova, M. V.

doi:10.1007/s11177-005-0072-2

Cited by 9 publications

(2 citation statements)

References 10 publications

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“…Previous studies have reported reduced allozyme and mitochondrial DNA variability in Kamchatkan populations (the north-western edge of the species' range) compared with North America (Osinov & Pavlov 1994;Pavlov 2000;Pavlov et al 2004). We also found evidence for reduced microsatellite diversity in populations from Kamchatka.…”

Section: Comparability Of Kamchatka and Eastern Pacific O Mykiss Popmentioning

confidence: 97%

Population structure and partial anadromy in Oncorhynchus mykiss from Kamchatka: relevance for conservation strategies around the Pacific Rim

McPhee

Utter

Stanford

et al. 2007

Ecology of Freshwater Fish

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DS, Allendorf FW. Population structure and partial anadromy in Oncorhynchus mykiss from Kamchatka: relevance for conservation strategies around the Pacific Rim.Abstract -Conservation of life-history diversity found in Oncorhynchus mykiss requires knowledge of the underlying population structure and genetic basis of this variability. We analysed variation at 10 microsatellite loci from seven rivers across Kamchatka to identify population structure and to test for divergence between life-history forms. We found lower heterozygosity in Kamchatkan populations compared with North American populations, but population structure was substantial (region-wide F ST ¼ 0.11) and followed an isolation-by-distance pattern similar to that reported for older North American populations. We found no evidence for genetic divergence between resident and anadromous individuals in the Sopochnaya River or between typically anadromous individuals and 'half-pounders' in the Utkholok River. A review of other studies of reproductive isolation, in combination with our results, suggests: (1) that pristine populations of steelhead should be expected to exhibit partial anadromy; and (2) that managing anadromous and resident individuals separately without demonstrating reproductive isolation is biologically unsound.

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Section: Comparability Of Kamchatka and Eastern Pacific O Mykiss Popmentioning

confidence: 97%

Population structure and partial anadromy in Oncorhynchus mykiss from Kamchatka: relevance for conservation strategies around the Pacific Rim

McPhee

Utter

Stanford

et al. 2007

Ecology of Freshwater Fish

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“…This makes salmon a unique object for studying the problems of the origin, formation of the wild type population structure, mechanisms of microevolution, and speciation pro cesses. An important feature of the Kamchatka group of mykiss populations is the depletion of the genome in the Asian part of the range [1] and a small number of effective population genetic markers. The phyloge netic subdivision of the Kamchatka mykiss popula tions was studied insufficiently and primarily by our group of authors.…”

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confidence: 99%

Introduction of ISSR markers for Kamchatka mykiss (Parasalmo (Oncorhynchus) mykiss) (Walbaum) (Salmonidae, Salmoniformes)

Melnikova

Senchukova

Павлов

2014

Dokl Biochem Biophys

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Pacific noble salmons of the genus Parasalmo (Oncorhynchus) have a latitudinal amphipacific distri bution and exhibit a high level of variations in the behavioral, morphological, and ecological features, which determine the wide range of life strategies and intraspecific forms. The species Parasalmo (Oncorhyn chus) mykiss (rainbow trout, mykiss) is one of the most interesting members of this genus showing a high adaptability, morphological plasticity, and an extremely wide distribution. In the Asian part of the range, the mykiss is of particular scientific and practi cal interest, since here this species is represented pri marily by the wild type populations that have retained their original population structure. This makes salmon a unique object for studying the problems of the origin, formation of the wild type population structure, mechanisms of microevolution, and speciation pro cesses. An important feature of the Kamchatka group of mykiss populations is the depletion of the genome in the Asian part of the range [1] and a small number of effective population genetic markers. The phyloge netic subdivision of the Kamchatka mykiss popula tions was studied insufficiently and primarily by our group of authors. In the previous studies, the geo graphical differentiation was determined using alloz ymes [2], SCAR markers developed in our laboratory [3], and microsatellite locus analysis [4]. The aim of the present study was to develop new techniques and increase the number of such population significant genetic mark ers by analyzing poorly studied DNA regions.The high variability, which can be used in the pop ulation analysis, is characteristic of the inter satellite DNA loci (ISSR PCR, inter microsatellite PCR). This method has been widely used in the practice of multilocus mapping of the genome since 1994 [5]. This method has a high resolution and is used in phy logenetics mainly at the level of populations and closely related species [6]. However, this method has not been used earlier to study the population genetic relationships of the Kamchatka mykiss. Earlier, we have created seven original ISSR markers specifically for this fish species [7]. In the present study, we evalu ated the efficiency of using these markers for genetic differentiation of myukiss populations in Kamchatka.The study was performed on the same material ( Table 1) that was used in the previous study per formed with SCAR markers [3].The DNA isolation method, the tests for determi nation of DNA quantity and quality, the selection of ISSR primers, and PCR conditions were described in detail in our methodical work on the development of ISSR markers [7]. The reproducibility of results in dif ferent experiments was tested three times using seven pairs of primers. When amplification reagents manu factured by the same company were used, the results were reproduced in 100% of cases.The frequency of null alleles was calculated by the formula proposed by L.A. Zhivotovsky for diploid objects studied using multilocus dominant DNA markers [8]. The allelic d...

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Regularities of Formation of Diversity of Life Strategy and Genetic Variability of The Kamchatka Rainbow Trout Parasalmo mykiss in a Local Population

et al. 2020

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Genetic divergence of mykizha (Parasalmo (Oncorhynchus) mykiss) from Kamchatka inferred from restriction analysis and sequencing of mtDNA cytochrome b gene

Cited by 9 publications

References 10 publications

Population structure and partial anadromy in Oncorhynchus mykiss from Kamchatka: relevance for conservation strategies around the Pacific Rim

Population structure and partial anadromy in Oncorhynchus mykiss from Kamchatka: relevance for conservation strategies around the Pacific Rim

Introduction of ISSR markers for Kamchatka mykiss (Parasalmo (Oncorhynchus) mykiss) (Walbaum) (Salmonidae, Salmoniformes)

Regularities of Formation of Diversity of Life Strategy and Genetic Variability of The Kamchatka Rainbow Trout Parasalmo mykiss in a Local Population

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