Genetic diversity among elite chickpea (Cicer arietinum L.) varieties of Ethiopia based on inter simple sequence repeat markers

Abstract

The inter simple sequence repeat (ISSR) marker was used in this study for genetic fingerprinting and identification of released chickpea varieties and for the determination of the genetic relationships among these cultivars. A total of 19 released chickpea varieties were subjected to ISSR analysis with the objective of evaluating the genetic diversity among the cultivars. A total of 20 ISSR primers were initially screened and later based on their reproducibility and polymorphism, four of them were selected for the molecular diversity assay. Amplification of genomic DNA of the 19 varieties using four ISSR primers produced 38 scorable fragments. On average, 9.5 polymorphic bands per primer were observed in ISSR analysis. The total number of bands amplified by 3' anchored primers varied from 7 to 12. The primers based on poly (GGAGA) and (AG)YT repeat motifs produced highest number of fragments (10 and 12, respectively), whereas, primers GACA and (GA)T, produced minimum number of fragments (7 and 9, respectively). Overall, 81.58% of the loci were polymorphic and 96.17 and 3.83% of variation were partitioned into within and among population, respectively. The least genetic similarity was recorded between the varieties, Shasho and Minjar (0.41), and highest genetic similarity (0.97) between the varieties, Worku and Kutaye. The UPGMA dendrogram clustered all genotypes into four different clusters and three varieties such as Shasho, Chefe and DZ-10-11 observed to be an out lie. The results indicate the presence of genetic diversity within released cultivars of chickpea in Ethiopia.

Genetic Diversity Analysis of Ethiopian Elite Chickpea (Cicer arietinum L.) Varieties Based on Agronomic Characters

“…The report is supported by other authors who reported the presence of genotypic variability in chickpea; such as Kumar et al (1999), Nimbalkar (2000), Wahid and Ahmad (1999) who observed significant variation for a number of seeds per pod and per plant, seeds per plant, hundred seed weight and yield per plant, respectively. Several chickpea investigations recorded significant genotypic differences among the crop collections studied by them (Chander et al, 2001;Abdalla et al, 2003;Zerihun et al, 2018).…”

Seed germination of seeds preserved for about 20 years and that of seeds preserved for several years

“…There are several reports about genetic diversity, germplasm or seed of plant (Admas and Tesfaye, 2017;Ali et al, 2018;Arantes et al, 2018;Chalachew et al, 2017;Chandra et al, 2017;Daniel et al, 2017;Dilooshi et al, 2016;Santos et al, 2017;Ejigu et al, 2018;Emmanuel et al, 2018;Isaac et al, 2016;Lopez-Puc and Rodriguez-Buenfil, 2017;Revolti et al, 2018;Miccah et al, 2016;Mohammad et al, 2016;Mounawer et al, 2016;Mulima et al, 2018;Ochieng et al, 2015;Pacôme et al, 2016;Raimundo et al, 2015;Rodrigues et al, 2016;Tadesse et al, 2018;Titilayo et al, 2018;Zerihun et al, 2018), fungi (Edelvio, 2018;Ling et al, 2016;Shubha and Srinivas, 2017), bacteria (Chaohe et al, 2015;Paul et al, 2016), and virus (Chikoti et al, 2015).…”