Genetic diversity among Japanese local populations of an edible and medicinal coastal plant Glehnia littoralis F. Schmidt ex Miq.

Tamura, Yasuhiro; Kubo, Nakao; Ohsako, Takanori

doi:10.1007/s10722-021-01205-5

Cited by 9 publications

(6 citation statements)

References 49 publications

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“…The soil and climatic conditions of the study area have bred rich and unique halophytes in the coastal zones, such as Salicornia europaea , V. rotundifolia , G. littoralis , C. soldanella , Apocynum venetum ( Bian, Li & Zhang, 2007 ). Halophytic vegetation is widely distributed in the coastal saline soil, and is important for wind mitigation and sand stabilization; some halophytes also have high medicinal value ( Zhao & Fang, 2005 ; Bian, Li & Zhang, 2007 ; Tamura, Kubo & Ohsako, 2021 ). Therefore, studying the effects of salt stress and nitrogen supply on halophytes is of great significance for improving the ecological environment and economic development of the coastal zones.…”

Section: Methodsmentioning

confidence: 99%

Effect of salt stress and nitrogen supply on seed germination and early seedling growth of three coastal halophytes

Chen

Liu

Zhang

et al. 2022

PeerJ

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Due to high salinity and low nutrient concentrations, the coastal zone is considered as one of the most vulnerable of the earth’s habitats. Thus, the effect of salt and nitrogen on growth and development of coastal halophytes has been extensively investigated in recent years, but insufficient attention has been paid to the crucial stages of plant establishment, such as seed germination and seedling growth. Thus, we carried out a field experiment to evaluate the effects of salt stress (6, 10 and 20 g/kg NaCl) and nitrogen supply (0, 6 and 12 gm−2year−1) on seed germination and seedling growth of three coastal halophytes (including two dominant herb species Glehnia littoralis and Calystegia soldanella, one constructive shrub species Vitex rotundifolia) from September 2020 to June 2021. The results of our experiment showed that seeds of G. littoralis exhibited an explosive germination strategy in the early spring of 2021 with 70% of the seeds germinating. Conversely, the seeds of V. rotundifolia exhibited slow germination in the late spring of 2021 with only 60% of the seeds germinating. C. soldanella seed germination exhibited two obvious peak periods, but only 6% of the seeds germinated, which means that most seeds may be stored in the soil by stratification or died. All three halophytes showed greater sensitivity to nitrogen than salt stress during the seed germination stage. Nitrogen supply significantly delayed seed germination and reduced the cumulative germination percentage, particularly for G. littoralis. Despite the large impact of nitrogen on seed germination, nitrogen had a larger impact on seedling growth suggesting that the seedling growth stage of halophytes is more vulnerable to changes in nitrogen supply. Moreover, nitrogen supply significantly reduced the individual biomass of G. littoralis, C. soldanella and V. rotundifolia, with greater decreases seen in the dominant species than in the constructive species. Conversely, nitrogen supply increased underground biomass allocation of G. littoralis and C. soldanella, suggesting that the constructive species were less sensitive to nitrogen and exhibited a stronger anti-interference ability than the dominant species. Therefore, increasing nitrogen supply may firstly affect the seed germination and seedling growth of the dominant species, but not the constructive species.

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Section: Methodsmentioning

confidence: 99%

Effect of salt stress and nitrogen supply on seed germination and early seedling growth of three coastal halophytes

Chen

Liu

Zhang

et al. 2022

PeerJ

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“…The dried roots of G. littoralis , generally called “Bei-Sha-Shen” in China, has high medicinal value for it is usually used in the treatment of several diseases as an important traditional Chinese medicine [ 1 ]. In addition, its young leaves and fresh roots are utilized as a vegetable [ 2 , 3 ]. It has been reported that coumarins, polyacetylenes, lignans, polysaccharides and flavonoids have been isolated from the extracts of G. littoralis , which have immunoregulation, anti-inflammatory，antioxidant, antibacterial, antitumor and anticancer activities [ 2 , 4 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

Sporogenesis, gametophyte development and embryogenesis in Glehnia littoralis

Zhou

Zhang

et al. 2023

BMC Plant Biol

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Background Glehnia littoralis is an economic herb with both medicinal and edible uses. It also has important ecological value and special phylogenetic status as it is a monotypic genus species distributing around beach. Little information on its reproductive biology has been reported so far, which has hindered conservation and application of this species. In this study, we observed morphological changes from buds emergence to seeds formation and internal changes during sporogenesis, gametophyte development and embryo and endosperm development of G. littoralis using paraffin-embedded-sectioning and stereo microscope. Results The results showed that the stages of internal development events of G. littoralis corresponded to obvious external morphological changes, most of developmental features were consistent with other Apiaceae species. The development of male and female gametophytes was not synchronized in the same flower, however, exhibited temporal overlap. From mid-late April to mid-May, the anther primordial and ovule primordial developed into the trinucleate pollen grain and eight-nuclear embryo sac, respectively. From late-May to mid-July, the zygote developed into mature embryo. In addition, some defects in gynoecium or ovule development and abnormal embryo and endosperm development were found. We induced that the possible causes of abortion in G. littoralis were as follows: nutrient limitation, poor pollination and fertilization, and bad weather. Conclusions This study revealed the whole process and morphological characteristics of the development of reproductive organ in G. littoralis, which not only provided important data for the study of systematic and conservation biology, but also provided a theoretical basis for cross breeding.

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“…SSR markers have been widely used in variety identification, fingerprinting construction and intraspecific genetic diversity analysis [14,25,34]. The selection of a set of core SSR primers for germplasm identification and genetic diversity have been conducted for many medicinal plants, such as Glehnia littoralis [27], Glycyrrhiza [16], and Euryale ferox [15]. However, such a marker toolkit is not presently available for A. chinensis genetic diversity analysis.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-derived SSR markers for DNA fingerprinting and inter-populations genetic diversity assessment of Atractylodes chinensis

Zhao²,

Su³

et al. 2022

Nucleus

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Atractylodes chinensis (fam. Asteraceae) is an important medicinal plant due to its unique pharmacological activity. The species is widely distributed in most areas of northern China. It is difficult to identify different populations of A. chinensis due to their similarity in characteristics. This study was the first investigation to date that assessed the genetic diversity of A. chinensis from different geographical counties of northern China using simple sequence repeat (SSR) markers. Of the 106 SSR primers in the clusters classified in the sesquiterpenoid biosynthesis pathway in the transcriptomic database of A. chinensis, ten with high polymorphism were used to analyze the inter-populations genetic diversity and construct DNA fingerprinting of 19 A. chinensis populations. A total of 78 alleles were detected, with an average number of 6.5 alleles per primer. The PIC value ranged from 0.4748 to 0.8918 with a mean of 0.6265. The neighbor-joining tree was used to classify 19 populations of A. chinensis into three clusters. DNA fingerprinting was performed according to these ten SSR markers. The results revealed that geographic origin is not exactly related to genetic diversity, as populations belonging to different provinces are grouped in the same cluster. The results of this study confirm that SSR markers are effective for genetic diversity analysis. The inter-populations genetic diversity and fingerprinting of A. chinensis in this study could provide a scientific basis for species identification and selective breeding.

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Genetic diversity among Japanese local populations of an edible and medicinal coastal plant Glehnia littoralis F. Schmidt ex Miq.

Cited by 9 publications

References 49 publications

Effect of salt stress and nitrogen supply on seed germination and early seedling growth of three coastal halophytes

Effect of salt stress and nitrogen supply on seed germination and early seedling growth of three coastal halophytes

Sporogenesis, gametophyte development and embryogenesis in Glehnia littoralis

Transcriptome-derived SSR markers for DNA fingerprinting and inter-populations genetic diversity assessment of Atractylodes chinensis

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