Genetic diversity analysis among collected purslane (Portulaca oleracea L.) accessions using ISSR markers

Alam, Md. Amirul; Juraimi, Abdul Shukor; Rafii, Mohd Y.; Hamid, Azizah Abdul; Arolu, Ibrahim Wasiu; Latif, Abdul

doi:10.1016/j.crvi.2014.10.007

Cited by 18 publications

(12 citation statements)

References 37 publications

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“…Interestingly, the populations from Perak and Terengganu were much closer despite the great distance between them. These values of Nei's genetic distance were low compared to other plants of different families such as Portulaca oleracea [49], in which a comparatively greater genetic distance was reported.…”

Section: Discussionmentioning

confidence: 82%

Genetic Diversity of Torch Ginger (Etlingera elatior) Germplasm Revealed by ISSR and SSR Markers

Ismail

Rafii

Mahmud

et al. 2019

BioMed Research International

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Fifty-seven accessions of torch ginger (Etlingera elatior) collected from seven states in Peninsular Malaysia were evaluated for their molecular characteristics using ISSR and SSR markers to assess the pattern of genetic diversity and association among the characteristics. Diversity study through molecular characterization showed that high variability existed among the 57 torch ginger accessions. ISSR and SSR molecular markers revealed the presence of high genetic variability among the torch ginger accessions. The combination of different molecular markers offered reliable and convincing information about the genetic diversity of torch ginger germplasm. This study found that SSR marker was more informative compared to ISSR marker in determination of gene diversity, polymorphic information content (PIC), and heterozygosity in this population. SSR also revealed high ability in evaluating diversity levels, genetic structure, and relationships of torch ginger due to their codominance and rich allelic diversity. High level of genetic diversity discovered by SSR markers showed the effectiveness of this marker to detect the polymorphism in this germplasm collection.

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Section: Discussionmentioning

confidence: 82%

Genetic Diversity of Torch Ginger (Etlingera elatior) Germplasm Revealed by ISSR and SSR Markers

Ismail

Rafii

Mahmud

et al. 2019

BioMed Research International

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“…Farsani et al (2012) found PIC values ranging from 0.26 to 0.46 in the characterization of Bermudagrass (Cynodon dactylon). Alam et al (2015) reported values varying from 0.22 to 0.37 in their molecular study on purslane (Portulaca oleracea L.) using ISSR markers. Similarity coefficient between the studied accessions varied from 0.462 to 0.962, with an average of 0.726.…”

Section: Discussionmentioning

confidence: 99%

“…The Cluster analysis was done to group the genotypes into a dendrogram. The dendrogram was constructed on the basis of the similarity matrix data using the unweighted pair group method, arithmetic averages (UPGMA) and numerical taxonomy system (NTSYS-pc) software (Rohlf et al, 2002;Alam et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…According to findings of this research, we can deduce that ISSR primers were informative markers and allowed us to detect considerable polymorphism in our genetic materials, suggesting that they could be very useful in safflower germplasm characterization and fingerprinting purposes. The genetic diversity study is very important for the choice of parents to be crossed in breeding program (Alam et al, 2015). An effective germplasm assessment provides scientific basis for breeding safflower for specific agro-ecological conditions.…”

Section: Figmentioning

confidence: 99%

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Genetic Diversity Analysis of Safflower (Carthamus tinctorius) Accessions from Different Geographic Origins using ISSR Markers

Houmanat¹,

Charafi²,

Mazouz³

et al. 2016

IJAB

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Safflower (Carthamus tinctorius) is an oilseed crop with high quality vegetable seed oil. A set of 55 accessions from different geographical origins were introduced to constitute a breeding program germplasm. These materials were characterized by inter-simple sequence repeat (ISSR) as a simple method to reveal polymorphism among them. ISSR analysis was carried out with 13 primers. After agarose gels analysis, ISSR banding patterns were converted into binary data of presence-non presence and matrices were processed. Genetic distances between all pairwise combinations of the accessions were calculated using similarity of simple coefficient matching (SCM). Dendrogram of studied accessions was constructed using the UPGMA (unweighted pair group method arithmetic average) method. A high number of reproducible ISSR bands exhibited a high percentage of polymorphism (69.64%), indicating a high genetic diversity among the material studied. The lowest similarity coefficient was 0.462, observed between accessions 109 and 135, whilst the highest one was 0.962, observed between accessions 68 and 126, with an average of 0.726. Dendrogram similarity relationships revealed some clusters according to different genetic pools. The ISSR markers were very informative and exhibited a high level of polymorphism. The findings of this study would be useful for safflower breeding program in Morocco as well as in other countries of the world.

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“…Besides to the above referred bioactive constituents, different types of alkaloids (oleraceins A-E, betalain alkaloid pigmentsreddish betacyanins and yellow betaxanthins), mucilages and pectins, flavonoids (apigenin, kaempferol, luteolin, quercetin, myricetin, genistein, genistin, portulacanones A-D), phenolic acids (caffeic, chlorogenic, p-coumaric, ferulic and rosmarinic acids), lignins, stilbenes, terpenoids, saponins, tannins, chlorophyll, bergapten and robustin have been also detected (Erkan, 2012). Finally, it has to be mentioned that the chemical composition of purslane shows a seasonal variation, while it also varied depending on growing and harvesting conditions and plant part (Alam et al, 2015).…”

Section: Chemical Compositionmentioning

confidence: 99%

Edible halophytes of the Mediterranean basin: Potential candidates for novel food products

Petropoulos

Karkanis

Martins

et al. 2018

Trends in Food Science & Technology

132

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Background: Recent trends in the food science industry and consumers' preferences for diversified diets suggest the consumption of wild greens not only as diet complements but also as healthy and functional foods for targeted conditions, rendering its commercial cultivation of major importance in order to avoid irrational gathering and genetic erosion threats. The Mediterranean basin abounds in wild edible species which have been used for food and medicinal purposes by human throughout the centuries. Many of these species can be found near coastal areas and usually grow under saline conditions, while others can adapt in various harsh conditions including high salinity. Scope and approach: The aim of this review focuses on listing and describing the most important halophyte species that traditionally have been gathered by rural communities of the Mediterranean basin, while special interest will be given on their chemical composition and health promoting components. Cases of commercially cultivated halophytes will be also presented to highlight their potential as alternative cash crops, while results from in vitro and in vivo health effects will be presented. Key findings and conclusions: The recent literature has provided useful information regarding the potential of wild halophytes as promising ingredients in functional food products and/or as sources of bioactive compounds. However, further research is needed regarding the chemical characterization of these species under commercial cultivation practices, while further clinical and model trials have to be conducted to assess their long term bioactivity and elucidate potential toxic effects and regulations of safe consumption.

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Genetic diversity analysis among collected purslane (Portulaca oleracea L.) accessions using ISSR markers

Cited by 18 publications

References 37 publications

Genetic Diversity of Torch Ginger (Etlingera elatior) Germplasm Revealed by ISSR and SSR Markers

Genetic Diversity of Torch Ginger (Etlingera elatior) Germplasm Revealed by ISSR and SSR Markers

Genetic Diversity Analysis of Safflower (Carthamus tinctorius) Accessions from Different Geographic Origins using ISSR Markers

Edible halophytes of the Mediterranean basin: Potential candidates for novel food products

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