Genetic diversity analysis and DNA fingerprinting of tomato breeding lines using SSR markers

Al-Shammari, Aziz Mahdi Abd; Hamdi, Ghassan Jaafar; Al-Mahdawi, Muthanna Abdulkhader Salh; Mohammed, Noora Khalifa

doi:10.15159/jas.21.13

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…The results indicate that F1 heterosis may not directly correlate with parental performance. Heterosis depends on the nature of genetic variation (Al-Shammari & Hamdi, 2021). However, heterosis may depend on the sum of the parents' GCA and SCA.…”

Section: Discussionmentioning

confidence: 99%

“…In comparison to other types of crops, 90-95 % of the land used for tomato production comprises hybrid varieties, which are preferred over pure line varieties due to their superiority in marketable yield and fruit quality, which are more resistant to biotic and abiotic challenges (Al-Shammari & Hamdi, 2021). The utilization of heterosis proves to be an efficient approach for improving tomatoes, as hybrid varieties exhibit high yield potential and have gained popularity among growers (Alwan & Mohammed, 2023).…”

Section: Introductionmentioning

confidence: 99%

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Impact evaluation combining ability of tomato hybrids and lines for production and fruit quality traits

Essa,

Alwan

2024

Agron. Mesoam.

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Introduction. The selection of pure lines followed by crosses to create superior hybrids is one of the oldest strategies in crop breeding. Combining ability studies provide reliable information for selection of parents for hybrid combination by revealing the nature and magnitude of gene actions involved in expression of quantitative traits. Objective. To estimate the General and Specific Combining Ability (GCA and SCA) values for yield in cherry tomatoes and select F1 hybrids combining high yield and fruit quality traits. Materials and methods. The half-diallel design, following method 2 of Griffing was used to define the best breeding method. Five lines were crossed to produce 10 hybrid combinations using a half diallel design involving the parents. Crosses and evaluations were conducted in a greenhouse in the Experimental Station at the University of Diyala, Baqubah, Iraq, in the winter of 2022-2023. Results. Based on GCA effects, lines 'LA353', 'LA3899' and 'IQ2' could be potential donor parents in breeding. Based on average performance, heterosis manifested and SCA effects, crosses IQ2×LA4013, LA3899×LA353 and IQ2×LA3899 were best in respect to yield and fruit quality. Conclusion. The general and specific combining ability values for the majority of the traits supported the material's great variety. The magnitude of heterosis was improved compared to parental lines. The high-yielding parent lines 'LA3899' and 'IQ2' could be used for breeding cherry tomatoes under greenhouse conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact evaluation combining ability of tomato hybrids and lines for production and fruit quality traits

Essa,

Alwan

2024

Agron. Mesoam.

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“…With all SSR markers was obtained a lower number of detectable bands, sometimes weak, or no bands for the last three plants (number 8, 9 and 10) acclimatized to ex vitro conditions (Figure 4). SSRs have been the most widely used markers for genotyping plants over the past 25 years because they are highly informative, codominant, multi-allele genetic markers that are experimentally reproducible and transferable among related species (Gheorghe et al, 2010;Shirasawa et al, 2014;Aguirre et al, 2017;Al-Shammari and Hamdi 2021). However, when using this type of molecular marker to distinguish between various genotypes, we must consider that SSRs have a high mutation rate.…”

Section: Morphological Aspects Comparisons Between Regenerated and Do...mentioning

confidence: 99%

Androgenic ability and plant regeneration potential in some tomato varieties

Bădulescu

Dumitru

Manolescu

et al. 2022

Not Bot Horti Agrobo

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Aiming to evaluate the in vitro regeneration potential, five varieties of tomatoes (Solanum lycopersicum) were studied for their response in anther culture. Anther explants at an early stage of microspore development were inoculated onto three culture media. The first differentiation processes were recorded during the first three weeks of culture, in darkness. The statistical analysis of the data recorded during the anther culture showed significant differences between genotypes regarding their specific response to culture conditions and the significant influence of the initiation medium composition in triggering the differentiation processes. Under the tested conditions were induced: the embryogenic potential in three genotypes (ʻȘtefănești 22ʼ, ʻCostate 21ʼ and ʻChihlimbarʼ) and the indirect organogenesis in two genotypes (ʻArgeș 20ʼ and ʻCostate 21ʼ). Morphological characteristics of anther-derived plants from ʻArgeș 20ʼ variety, grown in greenhouse conditions (growth rate, features of leaf, flower, and fruit), as well as analyses with nine SSR markers (banding patterns, the coefficient of genetic similarity, and the polymorphism information content) in DNA samples from each regenerant and the donor variety, provided clear evidence of the occurrence of spontaneous genetic variation during in vitro anther culture, and of the existence of somaclonal variation in regenerated plants. The amplified products obtained with SSR primers revealed a total number of scorable bands of 160 and a mean percentage of polymorphic bands of 21.09%. Two out of the nine tested primers, SSR63 and SLM6-7 proved to be efficient in detecting genetic differences not only among regenerants but also between them and the donor genotype.

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“…Not only is the in silico approach time and cost effective but it also allows for the discovery of SSRs from expressed sequence tags (ESTs) that represent the coding region of genome. SSR markers have been successfully utilized to analyze genetic diversity in tomato [ 20 , 21 , 22 , 23 ]. SSR marker are very crucial in breeding and analysis of plant abiotic stresses [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Development of SSR Markers Linked to Stress Responsive Genes along Tomato Chromosome 3 (Solanum lycopersicum L.)

Brake

Al-Qadumii

Hamasha

et al. 2022

BioTech

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This study aimed to develop novel SSR markers in tomato. Several BAC clones along chromosome 3 in tomato were selected based on their content. The criteria was the availability of genes, either directly or indirectly related to stress response (drought, salinity, and heat) in tomato. A total of 20 novel in silico SSR markers were developed and 96 important nearby genes were identified. The identified nearby genes represent different tomato genes involved in plant growth and development and biotic and abiotic stress tolerance. The developed SSR markers were assessed using tomato landraces. A total of 29 determinate and semi-determinate local tomato landraces collected from diverse environments were utilized. A total of 33 alleles with mean of 1.65 alleles per locus were scored, showing 100% polymorphic patterns, with a mean of 0.18 polymorphism information content (PIC) values. The mean of observed and expected heterozygosity were 0.19 and 0.24, respectively. The mean value of the Jaccard similarity index was used for clustering the landraces. The developed microsatellite markers showed potential to assess genetic variability among tomato landraces. The genetic distance information reported in this study can be used by breeders in future genetic improvement of tomato for tolerance against diverse stresses.

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Genetic diversity analysis and DNA fingerprinting of tomato breeding lines using SSR markers

Cited by 4 publications

References 13 publications

Impact evaluation combining ability of tomato hybrids and lines for production and fruit quality traits

Impact evaluation combining ability of tomato hybrids and lines for production and fruit quality traits

Androgenic ability and plant regeneration potential in some tomato varieties

Development of SSR Markers Linked to Stress Responsive Genes along Tomato Chromosome 3 (Solanum lycopersicum L.)

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