Genetic diversity analysis of Cynodon dactylon (bermudagrass) accessions and cultivars from different countries based on ISSR and SSR markers

Wang, Zhiyong; Liao, Li; Yuan, Xuejun; Guo, Hailin; Guo, Aizhen; Liu, Jianxiu

doi:10.1016/j.bse.2012.09.001

Cited by 40 publications

(33 citation statements)

References 23 publications

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“…ISSR refers to the amplification of a DNA region located between 2 microsatellite loci; this technique combines the advantages of random amplified polymorphic DNA markers with high polymorphism and reliability of microsatellites. This marker type has been successfully used for cultivar identification (Assefa et al, 2003;Wang et al, 2013), hybrid species discrimination (Wolfe and Randle, 2001;Chung et al, 2013), and genetic map construction, as well as intra-and interspecies genetic diversity and relationship determination in closely related taxa (Bodo Slota and Porter, 2006;Galván et al, 2010;Li et al, 2011). In this study, we examined genetic variation in P. tabulaeformis f. shekanensis compared with its nearest wild relatives to assess taxonomic status of P. tabulaeformis f. shekanensis and to propose new conservation policies for this rare taxon.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and taxonomic status of Pinus tabulaeformis f. shekanensis revealed by ISSR markers

Liu

Xie

2015

Genet. Mol. Res.

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ABSTRACT. Pinus tabulaeformis f. shekanensis is a rare taxon endemic in the Ziwuling Loess Plateau, of which only one population is known. Inter-simple sequence repeat molecular markers were employed to compare the taxon's genetic diversity with its 4 nearest wild relatives (P. tabulaeformis, P. tabulaeformis var. mukdensis, P. massoniana, and P. henryi) to assess the taxonomic status of P. tabulaeformis f. shekanensis. Inter-simple sequence repeat marker data revealed higher genetic diversity in the P. tabulaeformis f. shekanensis population than in the other populations. Population genetic analysis (neighbor-joining cluster analysis, principal coordinate analysis, and structure clustering) revealed that P. tabulaeformis f. shekanensis and P. tabulaeformis are likely conspecific (the former may be a variety of the latter). Strategies are also proposed for the conservation of P. tabulaeformis f. shekanensis.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity and taxonomic status of Pinus tabulaeformis f. shekanensis revealed by ISSR markers

Liu

Xie

2015

Genet. Mol. Res.

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“…This approach is highly reproducible and has relatively low cost (Bornet and Branchard, 2001). ISSR analysis has been used to assess the genetic diversity of a large number of plant species, including Lolium spp (Dinelli et al, 2004) and Cynodon dactylon (Wang et al, 2013). Both SSRs and ISSRs are powerful and useful tools for genetic studies and have been used in several plant species; however, at present, reports remain P. Madesis et al limited about their use in studies of D. glomerata populations (Litrico et al, 2009;Xie et al, 2010Xie et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and structure of natural Dactylis glomerata L. populations revealed by morphological and microsatellite-based (SSR/ISSR) markers

Madesis¹,

Abraham²,

Kalivas³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Dactylis glomerata L. is an important forage species in the Mediterranean region, and in other regions with a similar climate. Genetic material from 3 locations in north, central, and south Greece was studied, using morphological traits, SSR, and ISSR molecular markers. Morphological analysis revealed differences among the geographic locations studied for all morphological traits, except the number of reproductive tillers. Moreover, the highest phenotypic variation was observed on the accessions from south, while the lowest was observed on the accessions from the north. Although the results of the molecular marker analysis are indicative, a high level of Morphological and genetic diversity of Dactylis glomerata genetic diversity at the species level was revealed by ISSRs (G ST = 0.291) and SSRs (F ST = 0.186). Analysis of molecular variance showed that a high level of genetic diversity existed for ISSRs and SSRs within populations (62 and 83%, respectively), rather than among populations (38 and 17%, respectively). Cluster analysis divided the 3 populations in 2 groups, with the population originating from the island of Crete forming 1 group, while the populations from north Greece (Taxiarchis) and central Greece (Pertouli) were clustered in a 2nd group. In general, the results indicate that SSRs are more informative compared to ISSRs about the genetic variation within a population, whereas the ISSRs were more informative about the genetic diversity among populations However, a similar trend in diversity (genotypic and phenotypic) was observed in the morphological traits and microsatellite-based (SSR/ISSR) markers at the locations studied.

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“…Among the wide diversity of molecular markers, inter simple sequence repeat (ISSR) markers developed by Zietkiewicz et al (1994) have been used to detect genetic similarities or differences in plants (Gurkok et al 2013;Hassanpour et al 2013;Wang et al 2013;Hadian et al 2014). For phylogenetic analyses the method is mostly used to infer relationships within species and among species within subgeneric groups, as for example in Oryza sativa L. (Joshi et al 2000), wheat (Nagaoka and Ogihara 1997), Diplotaxis DC.…”

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Interspecific Relationships in Allium subgenus Melanocrommyum sections Acanthoprason and Asteroprason (Amaryllidaceae) Revealed Using ISSR Markers

et al. 2015

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Allium subgenus Melanocrommyum is taxonomically one of the most complicated groups within Allium and its sectional delimitation has been changed several times. In the present study, the relationships among 136 individuals representing 28 species of the sections Acanthoprason and Asteroprason were surveyed using ISSR (inter simple sequence repeats) markers. Nine ISSR primers generated 322 polymorphic bands (on average 36 bands per primer). In the resulting neighbor joining dendrogram, most conspecific individuals were grouped into clusters, which can be regarded as evidence of the monophyly of most species. Exceptions were the positions of six single samples of A. graveolens, A. zagricum, A. shelkovnikovii, A. subakaka, and A. materculae, which were not grouped with their remaining conspecific individuals. The clusters in dendrograms were significantly correlated with morphological similarities and biogeographical distributions. The lowest molecular similarity (62%) was observed between A. shelkovnikovii and A. ubipetrense and the most similar taxa (similarity value = 93%) were A. materculae and A. graveolens (sect. Acanthoprason). The results showed that ISSR analysis is suitable to detect the relationship of closely related species. The groups constructed agree only partly with those previously published based on molecular data, and represent alternative proposals to define taxonomic groups.

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Genetic diversity analysis of Cynodon dactylon (bermudagrass) accessions and cultivars from different countries based on ISSR and SSR markers

Cited by 40 publications

References 23 publications

Genetic diversity and taxonomic status of Pinus tabulaeformis f. shekanensis revealed by ISSR markers

Genetic diversity and taxonomic status of Pinus tabulaeformis f. shekanensis revealed by ISSR markers

Genetic diversity and structure of natural Dactylis glomerata L. populations revealed by morphological and microsatellite-based (SSR/ISSR) markers

Interspecific Relationships in <I>Allium</I> subgenus <I>Melanocrommyum</I> sections <I>Acanthoprason</I> and <I>Asteroprason</I> (Amaryllidaceae) Revealed Using ISSR Markers

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