Genetic diversity and distribution of a distinct strain of Chili leaf curl virus and associated betasatellite infecting tomato and pepper in Oman

Khan, A. J.; Akhtar, Sohail; Al-Zaidi, Amal M.; Singh, AK; Briddon, R. W.

doi:10.1016/j.virusres.2013.07.018

Cited by 45 publications

(41 citation statements)

References 57 publications

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“…The sequence of Tom‐127 was determined to be 1,373 nt and is available in the databases under accession numbers . Comparison of the sequences by BLASTn to all nucleotide sequences available in the databases showed the betasatellite sequence obtained to have the highest levels of nucleotide sequence identity (92.9%) to tomato leaf curl betasatellite (ToLCB) isolates ( and ) previously characterised from tomato in Oman (Khan, Akhtar, Al‐Zaidi, et al., ; Khan et al., ). These findings indicate that the betasatellite associated with the tomato plant infected by CLCuGeV is an isolate of ToLCB.…”

Section: Resultsmentioning

confidence: 95%

“…OLCOMB was identified in okra with the “Oman” strain of CLCuGeV (Akhtar et al., ). ToLCB is the most commonly encountered betasatellite in Oman, having been identified with a range of viruses including ChLCV, ToLCBrV, ToLCLwV and TYLCV (Al‐Shihi et al., ; Khan, Akhtar, Al‐Zaidi, et al., ; Khan et al., ). ToLCB occurs in Pakistan and India, from where it is believed to have been introduced into Oman (Khan, Akhtar, Al‐Zaidi, et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…ToLCB is the most commonly encountered betasatellite in Oman, having been identified with a range of viruses including ChLCV, ToLCBrV, ToLCLwV and TYLCV (Al‐Shihi et al., ; Khan, Akhtar, Al‐Zaidi, et al., ; Khan et al., ). ToLCB occurs in Pakistan and India, from where it is believed to have been introduced into Oman (Khan, Akhtar, Al‐Zaidi, et al., ). CLCuGeV infecting plants of the family Malvaceae is more commonly identified in the presence of CLCuGeB, a Malvaceae ‐adapted betasatellite (Idris, Briddon, Bull, & Brown, ; Kon, Rojas, Abdourhamane, & Gilbertson, ; Leke et al., ; Shih, Kumar, Tsai, Lee, & Green, ; Tiendrébéogo et al., ) that is occasionally also identified in non‐malvaceous hosts (Leke et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…The Al‐Batinah region produces 68,000–70,000 tons of tomato a year, which is 85% of the total output of the country (Ministry of Agriculture & Fisheries Statistics, 2010, http://www.maf.gov.om). Production of tomato in Oman has been shown to be affected by at least five begomoviruses— Tomato yellow leaf curl virus (TYLCV), Tomato leaf curl Sudan virus (ToLCSDV) , Tomato leaf curl Liwa virus (ToLCLwV; previously known as Tomato leaf curl Al‐Batinah virus), Tomato leaf curl Barka virus (ToLCBrV) and Chilli leaf curl virus (ChLCV) (Al‐Shihi et al., ; Brown et al., ; Khan, Akhtar, Al‐Zaidi, et al., ; Khan, Akhtar, Singh, & Briddon, ; Khan, Akhtar, et al., ). Significantly, the majority of these begomoviruses have their origins outside of Oman and have been introduced relatively recently (Khan, Mansoor, & Briddon, ).…”

Section: Introductionmentioning

confidence: 99%

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Identification of a distinct strain of Cotton leaf curl Gezira virus infecting tomato in Oman

Shihi

Al-Sadi

Deadman

et al. 2017

Journal of Phytopathology

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Tomato (Solanum lycopersicum) plants exhibiting yellowing, curling and stunting symptoms were identified in fields of the Tawoos Agricultural Systems, in Al‐Batinah in Oman. Cloning and sequencing of restriction endonuclease digested rolling circle amplified viral DNA identified a cotton begomovirus (family Geminiviridae) associated with the symptomatic tomato plants. Detailed analysis of complete sequences showed the virus to be a previously unknown strain of Cotton leaf curl Gezira virus (CLCuGeV) in association with the betasatellite Tomato leaf curl betasatellite (ToLCB). The new CLCuGeV strain, for which the name “Al Batinah” strain is suggested, has the greatest levels of sequence identity (91.9%) to an isolate of CLCuGeV recently reported from the neighbouring United Arab Emirates. Additionally, CLCuGeV‐Al Batinah was shown to have a recombinant origin with sequences donated by an African cassava mosaic virus‐like parent. This is the first identification of this Malvaceae‐adapted begomovirus in tomato. Although ToLCB is common in Oman, being one of only two betasatellites identified there so far, this is the first identification of this betasatellite with CLCuGeV. The significance of these findings is discussed.

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Section: Resultsmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of a distinct strain of Cotton leaf curl Gezira virus infecting tomato in Oman

Shihi

Al-Sadi

Deadman

et al. 2017

Journal of Phytopathology

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“…The sequence identity and phylogenetic analysis results strongly support the causative agent is a variant of Tomato leaf curl Sudan virus (ToLCSDV), a virus that has been reported earlier to cause tomato leaf curl disease in Sudan, Oman and Yemen. The association of begomovirus with leaf curl disease on various crops in Arabian Peninsula has been published in many reports (Ajlan et al, 2007;Khan et al, 2008;Idris et al, 2012;Akhtar et al, 2014;Idris et al, 2011;Khan et al, 2013a;(Ajlan et al, 2007;Khan et al, 2008;Idris et al, 2012;Akhtar et al, 2014;Idris et al, 2011;Khan et al, 2013bAl-Saleh et al, 2014Hosseinzadeh et al, 2014;Idris et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Tomato Leaf Curl Sudan Virus (TLCSDV) causing leaf curl disease on a new host Amaranthus cruentus L.

Sohrab¹

2017

Plant Omics

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Amaranthus leaf curl disease symptom was observed in the farmer's field based at Jeddah, Saudi Arabia. This crop used as leafy vegetables in many countries. In this study, a field survey was conducted in April 2014 and naturally infected Amaranthus leaf samples were collected to identify the associated virus with leaf curl disease. The causative agent was transmitted through whiteflies (Bemisia tabaci) from naturally infected leaves to healthy Amaranthus seedlings. The begomovirus infection was identified by PCR by using specific primers. The full viral genome was amplified by rolling circle amplification. The presence of betasatellites was also confirmed by using betasatellites specific primers. The full viral genomes as well as betasatellites were amplified, cloned and sequenced. The full-length viral genome sequence analysis showed the highest (99.9%) homology with Tomato leaf curl Sudan Virus infecting tomato reported from the Arabian Peninsula. The betasatellites sequence analysis showed the highest identity (99.3%) with Tomato leaf curl betasatellites-Yemen. The phylogenetic analysis was performed by using both full as well as betasatellites genome and full genome formed the closest cluster with Tomato leaf curl Sudan virus while betasatellites genome formed closed cluster with tomato yellow leaf curl Yemen betasatellites. The recombination analysis was performed and results showed that the associated virus could be a variant of Tomato leaf curl Sudan virus, a virus that occurs in Sudan, Yemen and Arabian Peninsula. This is the first report that about the Tomato leaf curl Sudan virus causing leaf curl disease on a new host Amaranthus in Jeddah, Saudi Arabia.

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Geminivirus Occurrence in Australia, China, Europe, and the Middle Eastern Countries

Shihi

2019

Geminiviruses

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Genetic diversity and distribution of a distinct strain of Chili leaf curl virus and associated betasatellite infecting tomato and pepper in Oman

Cited by 45 publications

References 57 publications

Identification of a distinct strain of Cotton leaf curl Gezira virus infecting tomato in Oman

Identification of a distinct strain of Cotton leaf curl Gezira virus infecting tomato in Oman

Tomato Leaf Curl Sudan Virus (TLCSDV) causing leaf curl disease on a new host Amaranthus cruentus L.

Geminivirus Occurrence in Australia, China, Europe, and the Middle Eastern Countries

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