Genetic diversity and gene flow of some Persian walnut populations in southeast of Iran revealed by SSR markers

Vahdati, Kourosh; Pourtaklu, Somayeh Mohseni; Karimi, Rouhollah; Barzehkar, Rouhollah; Amiri, Rahebeh; Mozaffari, Mohammad; Woeste, Keith

doi:10.1007/s00606-014-1107-8

Cited by 59 publications

(58 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Accordingly, across the globe, walnut production can potentially contribute to improved food security. There is a strong possibility that parts of the world such as eastern Asia, northern America, southeastern Europe and Iran are centres of origin and possess a high percentage of the crop's genetic diversity (Vahdati et al , ).…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of walnut polyphenol oxidase gene (JrPPO1) in transgenic tobacco plants and PPO induction in response to walnut bacterial blight

et al. 2020

Self Cite

View full text Add to dashboard Cite

Walnut (Juglans regia) is economically important for both its wood and nut nutritional value, but it is susceptible to diseases such as walnut bacterial blight, caused by Xanthomonas arboricola pv. juglandis (Xaj). Walnuts contain many phenolic compounds, providing a good model on which to study polyphenol oxidase (PPO). We inoculated the detached walnut fruits of cultivars Ford, Chandler, Franquette, Robert Livermore, and Payne with Xaj and measured the induction of PPO activity in infected sites and adjacent to infected sites. Compared to infected and uninfected sites, PPO activity was induced significantly in areas adjacent to infected sites in all cultivars except Ford. Ford and Franquette, presenting the lowest and highest PPO activity, showed the largest and smallest mean diameter spots in response to Xaj, respectively. Polyacrylamide gel electrophoresis confirmed monophenol oxidase activity of walnut PPO in the assessed tissues. Then, we revealed the antipathogenic potential of walnut PPO through Agrobacterium tumefaciens‐mediated walnut JrPPO1 gene transfer into tobacco (Nicotiana tabacum). Two transformed tobacco lines overexpressing the JrPPO1 gene were regenerated successfully and challenged with Pseudomonas syringae pv. tabaci. Transgenic lines showed significantly higher PPO activity and lower disease severity to the pathogen compared to the control. However, a significant difference in disease severity and PPO activity level was observed between the two transgenic lines. Our results demonstrate a potential defence‐related role of PPO in transgenic tobacco and its induction in areas adjacent to infection sites in walnut cultivars treated with Xaj.

show abstract

Section: Introductionmentioning

confidence: 99%

Functional analysis of walnut polyphenol oxidase gene (JrPPO1) in transgenic tobacco plants and PPO induction in response to walnut bacterial blight

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…A considerable number of authors reported their results after applying sets of those SSR markers on different walnut samples and species (Dangle et al 2005;Victory et al 2006;Ross-Davis et al 2008;Ardhya et al 2010). The same SSR markers were also used for genetic research of national common walnut populations and cultivars in Europe (Foroni et al 2005;Foroni et al 2006;Foroni et al 2007;Cabral et al 2008;Pollegioni et al 2011;Ruiz Garcia et al 2011) and in Asia (Ahmed et al 2012;Gunn et al 2010;Ibrahimov et al 2010;Karimi et al 2010;Kim et al 2012;Mahmoodi et al 2013;Pollegioni et al 2014;Vahdati et al 2015;Wang et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)

Ćelepirović¹,

Vlahović²,

Dounavi³

et al. 2016

Silva Fenn.

View full text Add to dashboard Cite

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.) Ćelepirović N., Karija Vlahović M., Dounavi A., Ivanković M. (2016). Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.). Silva Fennica vol. 50 no. 5 article id 1674. 9 p. http://dx.doi.org/10.14214/sf.1674 Highlights• We combined eleven SSR markers in one multiplex PCR to make faster and cost effective amplification of the common walnut DNA from Croatia.• Genetic variation of common walnut from Croatia was moderate at analyzed SSR loci.• The resultant multiplex PCR could be used for genotyping of common walnut populations. AbstractMultiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube. In this study, we combined existing simple sequence repeat (SSR) markers (nuclear microsatellites) in the novel combination of multiplex PCR to study the population genetics of common walnut from Croatia. From twenty one tested SSR markers, eleven produced satisfactory results in one multiplex PCR. Population genetic results achieved from 15 samples of Croatian common walnut showed moderate genetic variability (average value: He 0.473; Ho 0.568). Our multiplex PCR allowed cost effective work concerning chemicals, plastic ware, device, and working time producing optimal results. The optimized multiplex PCR represented the best combination of eleven SSR primers for genotyping common walnut in a single PCR reaction.

show abstract

“…Los marcadores moleculares denominados microsatélites SSRs (Simple Sequence Repeats) son los marcadores más empleados en la actualidad en estudios de diversidad genética debido a su alta reproductividad, naturaleza multialélica, forma de herencia codominante y amplia cobertura del genoma (Schlötterer, 2004). Trabajos realizados en especies vegetales con dichos marcadores han aportado una cuantiosa información sobre diversidad genética en poblaciones de especies forestales como nogal (Vahdati et al, 2015), pino (Fady, 2012), roble (Steinkellner et al, 1997) y castaño (Martín et al, 2010;Mattioni et al, 2013;Lusini et al, 2014). Además, se han convertido en las mejores herramientas para los estudios de identificación varietal en especies vegetales o de huella genética (Varshney et al, 2005).…”

Section: Introductionunclassified

Influencia de la variedad del castaño en la incidencia de insectos carpófagos en las principales zonas productoras de castañas en Andalucía

Cuestas

Martín²,

Martín³

et al. 2016

Cuad. Soc. Esp. Cienc. For.

View full text Add to dashboard Cite

show abstract

Genetic diversity and gene flow of some Persian walnut populations in southeast of Iran revealed by SSR markers

Cited by 59 publications

References 28 publications

Functional analysis of walnut polyphenol oxidase gene (JrPPO1) in transgenic tobacco plants and PPO induction in response to walnut bacterial blight

Functional analysis of walnut polyphenol oxidase gene (JrPPO1) in transgenic tobacco plants and PPO induction in response to walnut bacterial blight

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)

Influencia de la variedad del castaño en la incidencia de insectos carpófagos en las principales zonas productoras de castañas en Andalucía

Contact Info

Product

Resources

About