Genetic diversity and pathogenicity of Fusarium oxysporum isolated from wilted rocket plants in Italy

Srinivasan, Kannupal; Spadaro, D.; Poli, A.; Gilardi, G.; Gullino, M. L.; Garibaldi, A.

doi:10.1007/s12600-011-0207-z

Cited by 20 publications

(12 citation statements)

References 43 publications

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“…The primers FIGS11/NPIGS-R were able to specifically amplify a DNA product from the Fo isolates that showed potential for controlling Fusarium wilt of cucumber (Table 1 and Fig 2). The variation in the intergenic spacer (IGS) region of ribosomal DNA is useful for resolving intra-specific relationships within Fo [23], [25], [26]. It has been suggested that the variation in the IGS of rDNA may have a considerable effect on development, evolution, and ecology through its effects on growth-rate regulation, resulting from the role of the IGS in the production of rRNA [41].…”

Section: Discussionmentioning

confidence: 99%

“…The intra-species diversity of several fungi, including formae speciales and races, has been further distinguished using the PCR approach [20], [21]. Recent studies showed that the intergenic spacer (IGS) region of ribosomal DNA (rDNA) is a source of phylogenetic markers in Fo [22], [23], [24], [25], [26] and that the region amplified with the general primers FIGS11/FIGS12 is suitable for the study of populations, including relationships among the Fo isolates [22], [27]. The objectives of this study were to identify polymorphisms in the IGS region of rDNA that differentiate nonpathogenic from pathogenic Fo isolates and to develop a method to assess the efficacy of nonpathogenic Fo isolates for use as potential biocontrol agents to manage Fusarium wilt of cucumber.…”

Section: Introductionmentioning

confidence: 99%

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Modified Primers for the Identification of Nonpathogenic Fusarium oxysporum Isolates That Have Biological Control Potential against Fusarium Wilt of Cucumber in Taiwan

Wang

Lin

et al. 2013

PLoS ONE

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Previous investigations demonstrated that Fusarium oxysporum (Fo), which is not pathogenic to cucumbers, could serve as a biological control agent for managing Fusarium wilt of cucumber caused by Fo f. sp. cucumerinum (Foc) in Taiwan. However, thus far it has not been possible to separate the populations of pathogenic Fo from the nonpathogenic isolates that have biological control potential through their morphological characteristics. Although these two populations can be distinguished from one another using a bioassay, the work is laborious and time-consuming. In this study, a fragment of the intergenic spacer (IGS) region of ribosomal DNA from an Fo biological control agent, Fo366, was PCR-amplified with published general primers, FIGS11/FIGS12 and sequenced. A new primer, NPIGS-R, which was designed based on the IGS sequence, was paired with the FIGS11 primer. These primers were then evaluated for their specificity to amplify DNA from nonpathogenic Fo isolates that have biological control potential. The results showed that the modified primer pair, FIGS11/NPIGS-R, amplified a 500-bp DNA fragment from five of seven nonpathogenic Fo isolates. These five Fo isolates delayed symptom development of cucumber Fusarium wilt in greenhouse bioassay tests. Seventy-seven Fo isolates were obtained from the soil and plant tissues and then subjected to amplification using the modified primer pair; six samples showed positive amplification. These six isolates did not cause symptoms on cucumber seedlings when grown in peat moss infested with the isolates and delayed disease development when the same plants were subsequently inoculated with a virulent isolate of Foc. Therefore, the modified primer pair may prove useful for the identification of Fo isolates that are nonpathogenic to cucumber which can potentially act as biocontrol agents for Fusarium wilt of cucumber.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modified Primers for the Identification of Nonpathogenic Fusarium oxysporum Isolates That Have Biological Control Potential against Fusarium Wilt of Cucumber in Taiwan

Wang

Lin

et al. 2013

PLoS ONE

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“…Attempts are made to replace them with molecular identification techniques to group isolates (Lievens et al, 2008;Pasquali et al, 2008). To better understand the genetic relationship between the formae speciales of F. oxysporum that have been already characterized and our isolates, it was useful to sequence the EF-1 and IGS regions (Srinivasan et al, 2012). Sequence analysis is very precise and reproducible to evaluate genetic similarity or identity, and can separate formae speciales of F. oxysporum better than RAPD, which was used in this work only to assess the similarity of the eleven FUSCIC.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Fusarium oxysporum f.sp. cichorii pathogenic on chicory (Cichorium intybus)

et al. 2012

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Fusarium oxysporum is a ubiquitous soilborne ascomycete responsible for vascular wilt in many plant species worldwide. This species comprises more than 120 putative host-specific formae speciales capable of causing remarkable economical losses. In summer 2009, wilt symptoms, including chlorosis and poor development of the root system were observed on cultivars of chicory (Cichorium intybus) in northern Italy. The causal agent isolated from symptomatic tissues in this case was identified as F. oxysporum on the basis of both morphological features and molecular analyses. In this work, we attempted to characterize the isolates of F. oxysporum from C. intybus by both biological and molecular approaches. Pathogenicity trials performed on 5 species of the Asteraceae family with isolates of F. oxysporum from C. intybus indicated that the pathogen has a unique host range, infecting chicory only. Neither lettuce nor endive, lawn daisy and Paris daisy developed the disease. Five cultivars within C. intybus species were tested, and the cv Clio was the most susceptible. Phylogenetic analyses relative to the ribosomal intergenic spacer (IGS) and translation elongation factor 1-alpha (EF1-) assigned isolates pathogenic to chicory to a single cluster, distinct from other pathogenic F. oxysporum. In light of these views, we propose to designate this organism as Fusarium oxysporum f.sp. cichorii.

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“…The two formae speciales that affect cultivated rocket, that is, Fusarium oxysporum ff.spp. raphani and conglutinans, the first of which is more frequently detected (Garibaldi et al, 2006;Srinivasan et al, 2012), also affect other genera belonging to Brassicaceae, such as cabbage, brussel sprouts, broccoli, turnip, radish and stock (Garibaldi et al, 2006). The Fusarium wilt of lettuce and rocket are easily and frequently seed-transmitted (Gullino et al, 2014), thus suggesting the importance of preventative disease management strategies.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the short term effect of nursery treatments with phosphite-based products, acibenzolar-S-methyl, pelleted Brassica carinata and biocontrol agents, against lettuce and cultivated rocket fusarium wilt under artificial inoculation and greenhouse conditions

et al. 2016

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Experimental trials have been carried out in order to evaluate the efficacy of preventative treatments based on plant defense activator products, biocontrol agents, a microbial complex with arbuscular mycorrhizal fungi, and Brassica carinata pellets against Fusarium oxysporum f. sp. lactucae race 1 on lettuce and Fusarium oxysporum f. sp. raphani on cultivated rocket under greenhouse conditions. These products were compared with fungicides known for their ability to induce host resistance (phosethyl-Al and acibenzolar-S-methyl), and with azoxystrobin. Three and four applications of the tested products were carried out on lettuce and rocket seedlings grown in nursery conditions. Treated and untreated plants were transplanted into soil infested with Fusarium wilt agents to obtain an average disease severity (DS) of 65.6-69.2 and of 56.9-62.1 on the untreated lettuce and rocket plants, respectively. The best Fusarium wilt biocontrol was obtained after four 2 applications of Bacillus subtilis Qst713 and with the Glomas microbial complex (42 and 46.7%, efficacy, respectively). Brassica carinata pellets provided a consistent control when applied 14 days before the rocket and lettuce were transplanted into the infested soil. Acibenzolar-S-methyl, applied at 0.025 g/liter, showed a DS reduction in F. oxysporum f. sp. lactucae from 36 to 61% and of F. oxysporum f. sp. raphani from 54 to 73%, thus showing statistically similar results to those of azoxystrobin, which was used as a reference (DS reduction from 59 to 65%). Although the Fusarium wilt control provided by such products was not complete in the present experimental conditions, these products can be considered interesting components for an integrated pest management of the Fusarium wilt of leafy vegetables, starting from nursey applications. Moreover, the tested BCAs could become potentially useful, especially for plant monocultures. This study has been produced new information on the effects of potassium phosphite, applied at the nursery level, on reducing lettuce and rocket fusarium wilt. An average efficacy of 69.5% was observed for lettuce, while an average efficacy of 65.2% was observed for cultivated rocket. The good fungicidal activity of the phosphite-based product, coupled with the positive effect on plant biomass, is of special interest.

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Genetic diversity and pathogenicity of Fusarium oxysporum isolated from wilted rocket plants in Italy

Cited by 20 publications

References 43 publications

Modified Primers for the Identification of Nonpathogenic Fusarium oxysporum Isolates That Have Biological Control Potential against Fusarium Wilt of Cucumber in Taiwan

Modified Primers for the Identification of Nonpathogenic Fusarium oxysporum Isolates That Have Biological Control Potential against Fusarium Wilt of Cucumber in Taiwan

Molecular characterization of Fusarium oxysporum f.sp. cichorii pathogenic on chicory (Cichorium intybus)

Evaluation of the short term effect of nursery treatments with phosphite-based products, acibenzolar-S-methyl, pelleted Brassica carinata and biocontrol agents, against lettuce and cultivated rocket fusarium wilt under artificial inoculation and greenhouse conditions

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