Genetic diversity and plant-growth related features of Burkholderia spp. from sugarcane roots

Luvizotto, Danice Mazzer; Marcon, Joelma; Andreote, Fernando Dini; Dini‐Andreote, Francisco; Neves, Aline A Camargo; Araújo, Welington Luiz; Pizzirani-Kleiner, Aline Aparecida

doi:10.1007/s11274-010-0364-0

Cited by 68 publications

(53 citation statements)

References 42 publications

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“…Genus Burkholderia sp. was identified in rice (Baldani et al, 2000;Oliveira et al, 2002), maize (Riggs et al, 2001) and sugarcane Luvizotto et al, 2010;Beneduzi et al, 2013). In South of Brazil, Beneduzi et al (2013) identified two species: Burkholderia phenazinium and Burkholderia cepacia, but in our study we found a different species (B. cenocepacia).…”

Section: Discussionmentioning

confidence: 40%

“…(Oliveira et al, 2002) and Burkholderia sp. Luvizotto et al, 2010). Some species were also found and described, such as: Bacillus polimixa (Rennie et al, 1982), Pantoea agglomerans (Loiret et al, 2004) and Gluconacetobacter diazotrophicus (Perin et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In sugarcane, the studies have focused on varieties cultivated in Southeast region of the country and have found important bacterial groups that perform BNF (Rennie et al, 1982;Oliveira et al, 2002;Loiret et al, 2004;Perin et al, 2004;Reis et al, 2004;Luvizotto et al, 2010;Costa et al, 2014). Resende et al (2006) reported that biological N 2 fixation (BNF) performed by bacteria associated with sugarcane can be responsible for up to 60% of the total N accumulated in the plants.…”

Section: Introductionmentioning

confidence: 99%

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Bacteria associated with sugarcane in Northeastern Brazil

Silva¹,

Freire²,

Kuklinsky-Sobral³

et al. 2016

Afr. J. Microbiol. Res.

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Sugarcane has a high demand in nitrogen, increasing costs and causing damages to the environment. It is necessary to find alternatives to reduce nitrogen fertilizers use. Diazotrophic bacteria have capacity to promote growth in grass with potential to fix N 2 and solubilize inorganic phosphate. This study aimed to evaluate bacterial community associated with different sugarcane varieties in Northeastern Brazil, select bacteria with plant growth-promoting characteristics, and identify endophytic and epiphytic bacterial lineages in sugarcane. Endophytic bacteria of leaves and roots and epiphytic bacteria of rhizoplane were isolated from three sugarcane commercial varieties and selected for their capacity to fix N 2 and solubilize inorganic phosphate. Bacterial strains from different morphological groups were isolated and a sample of 27 strains with potential for the simultaneous development of these characteristics were selected and identified. The bacterial community that interacted with sugarcane was more associated with rhizoplane and roots regions than with leaves, and had a high potential to fix N 2 and solubilize inorganic phosphate. Bacterial lineages were mainly from genera Pantoea sp. and Burkholderia sp., but there were also genera Enterobacter sp., Klebsiella sp. and Pseudomonas sp. and two lineages at the species level: Pantoea stewartii and Burkholderia cenocepacia.

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Section: Discussionmentioning

confidence: 40%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacteria associated with sugarcane in Northeastern Brazil

Silva¹,

Freire²,

Kuklinsky-Sobral³

et al. 2016

Afr. J. Microbiol. Res.

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“…This evidence is 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 characteristic of nitrogen-fixing bacteria (Marin et al, 2003;Ando et al, 2005;Luvizotto et al, 2010;Shu et al, 2012). Also, our results indicate that Poly PCR primers (Poly et al, 2001) were able to amplify nifH sequences of pineapple endophytes DNA; however, the amplification efficiency differed between them (Figure 2).…”

Section: Discussionmentioning

confidence: 71%

Identification of culturable endophytes in Champaka pineapple grown in an organic system

Weber¹,

Videira²,

Luiz³

et al. 2013

Afr. J. Agric. Res.

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The objective of the study was to characterize culturable diazotrophic endophytic bacteria from 'Champaka' pineapple plants during the fruiting period in an organic system. Micropropagated plants were inoculated with the diazotrophic endophytic bacterium, strain AB 219a, during acclimatization phase. In the field, the plants were fertilized with different dosages and sources of organic compost. After 17 months of field cultivation, roots and stems were collected for the isolation of endophytes, DNA extraction, 16S rDNA amplification and molecular analysis. A total of nine bacterial groups were identified, and species Burkholderia silvatlantica, Azorhizobium caulinodans, Pantoea eucrina, Erwinia sp. and unculturable bacterium occurred in inoculated plants. In contrast, non-inoculated plants were associated endophytes related to Burkholderia cenocepacia, Azospirillum brasilense, Enterobacter oryzae, Erwinia sp. and Sphingobium yanoikuyae. Segments of 360 base pair from nifH gene were amplified from representative endophytes within identified species, except from the Pantoea eucrina strain AB 295. 16S rDNA phylogenetic analysis of endophytes isolated from inoculated plants revealed distinct families: Xanthobacteraceae, Burkholderiaceae and Enterobacteriaceae; and from noninoculated plants, Rodospirillaceae and Sphingomonadaceae families were also identified. The 'Champaka' pineapple plants at fruiting stage associate with endophytes related to α, β and γ Proteobacteria, after plantlets inoculation or not with the diazotrophic bacterium Burkholderia silvatlantica (AB 219a). Most of those endophytes present nifH gene, a characteristic for nitrogen-fixing bacteria.

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“…These genera have been isolated from different plant hosts, indicating a wide host range. Enterobacteria have been isolates from citrus, cocoa, eucalyptus, soya bean, and sugarcane 27 , whereas Burkholderia are associated in roots of sugarcane 28,29 , rice 30 , and maize 29 . In bamboo, previous research found one species of Enterobacter and six species of Burkholderia from a total number of 56 species of bacteria from root tissue, rhizosphere, and rhizoplane 3 , and B. pyrrocinia is common to this study.…”

Section: Selection Of Phosphate-solubilizing Bacteriamentioning

confidence: 99%

Identification of phosphate-solubilizing bacteria from the bamboo rhizosphere

Ruangsanka¹

2014

ScienceAsia

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Some bacteria in the root zone of plants can solubilize phosphate making it available to the plant. Bacteria associated with bamboo roots may be a good source for development of bio-fertilizer for organic crop production. Here, 24 soil samples were collected from 8 species of bamboo, and 19 isolates of bacteria with the ability to solubilize phosphate were primarily selected based on the halo-to-colony ratio in Pikovskaya's medium. Five isolates with the highest ratios were cultured in vitro in a liquid medium containing mono-calcium phosphate (Ca(H 2 PO 4 ) 2 ) and soluble phosphate was then determined. The five isolates were identified using the full 16S rDNA sequencing method. Buttiauxella izardii had the highest soluble phosphate (134 mg/l) followed by Enterobacter cancerogenus (110 mg/l), Burkholderia ubonensis (106 mg/l), and E. hormaechei (103 mg/l), whereas Burkholderia pyrrocinia was lowest (38 mg/l). The isolates were further inoculated to soil planted with baby corn (Zea mays) in a pot experiment under an open environment. The experiment was not able to confirm the ability of these bacteria isolates to promote growth of the crop. The potential use of the isolates and the methods to improve the experiments for further studies are discussed.

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Genetic diversity and plant-growth related features of Burkholderia spp. from sugarcane roots

Cited by 68 publications

References 42 publications

Bacteria associated with sugarcane in Northeastern Brazil

Bacteria associated with sugarcane in Northeastern Brazil

Identification of culturable endophytes in Champaka pineapple grown in an organic system

Identification of phosphate-solubilizing bacteria from the bamboo rhizosphere

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