Genetic diversity and population structure of endangered<i>Glehnia littoralis</i>(Apiaceae) in China based on AFLP analysis

Li, Bin; Wang, Ailan; Ping, Zhang; Li, Weiwei

doi:10.1080/13102818.2019.1577172

Cited by 16 publications

(11 citation statements)

References 24 publications

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“…The genetic diversity coefficients were higher than or similar to those of other endangered species [9,11,[48][49][50]. This result was consistent with previous studies based on molecular markers and enzyme alleles [51][52][53][54][55]. Genetic diversity is the essence of species evolution and our results showed that G. littoralis has great evolutionary potential, which in turn, implies the contribution of severe habitat destruction on their endangered status.…”

Section: Genetic Structure and Genetic Diversity Analysis Revealed Bysupporting

confidence: 90%

Potential biological mechanisms underlying the endangered status of Glehnia littoralis revealed by nrDNA ITS and RAPD analyses

Zhang

et al. 2020

Biotechnology & Biotechnological Equipment

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Certain biological mechanisms of Glehnia littoralis, an endangered medicinal plant in China, may be underlying its endangered status and this has attracted extensive attention from researchers. In this study, nuclear ribosomal DNA internal transcribed spacer (nrDNA ITS) sequences and random amplified polymorphic DNA (RAPD) were investigated to determine the genetic diversity of G. littoralis. A total of 291 samples from 11 G. littoralis populations were collected from the east coast of China. The high genetic diversity and large genetic differentiation within populations were reconfirmed by both ITS (Ht ¼ 0.576, Hs ¼ 0.512, G st ¼ 0.1526) and RAPD analyses (Ht ¼ 0.51, Hs ¼ 0.35, G st ¼ 0.31). The phylogenetic trees showed consistent topologies based on ITS and RAPD; all populations were divided into two clades. Mantel tests (ITS: r ¼ 0.3296, p ¼ 0.067; RAPD: r ¼ 0.036, p ¼ 0.418) showed no significant positive correlation between the geographical distance and genetic distance for G. littoralis populations. These results showed that G. littoralis has great evolutionary potential and its status as endangered might be related to special dispersal and germination mechanisms of its seeds, as well as habitat destruction. In conclusion, we suggest that the conservation strategy for G. littoralis should be based on the protection of all the wild populations to the extent possible.

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Section: Genetic Structure and Genetic Diversity Analysis Revealed Bysupporting

confidence: 90%

Potential biological mechanisms underlying the endangered status of Glehnia littoralis revealed by nrDNA ITS and RAPD analyses

Zhang

et al. 2020

Biotechnology & Biotechnological Equipment

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“…AMOVA revealed that a majority of the total genetic diversity resides within populations. Studies on natural populations in China revealed that 77.8% to 84.2% of the total genetic variation occurred within populations (Wang et al 2016;Li et al 2019). These values indicated that the high proportion of the within-population component of genetic variation is a general tendency of the species.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity among Japanese local populations of an edible and medicinal coastal plant Glehnia littoralis F. Schmidt ex Miq.

Tamura

Kubo

Ohsako

2021

Genet Resour Crop Evol

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To assess the current genetic status of a useful coastal plant Glehnia littoralis F. Schmidt ex Miq. in Japan, the genetic diversity within and among natural populations was investigated. A total of 601 individuals from 32 local populations were genotyped using inter-primer binding site (iPBS) markers. In addition, most likelihood inference of the outcrossing rate was determined based on genotyping of maternal families from two populations using newly developed microsatellite markers. A total of 34 polymorphic bands were detected with seven iPBS primers. All populations showed genetic variation, with Shannon's I ranging from 0.577 to 0.107. Analysis of molecular variance (AMOVA), neighbor-joining analysis, and Bayesian cluster analysis revealed that the populations were differentiated into a group on the Sea of Japan coast and another on the Pacific Ocean coast. Furthermore, populations in geographic proximity were genetically more similar to each other. These results suggested that long-distance gene flow among local populations is limited. Nonetheless, a high within-population component of genetic diversity after AMOVA (77.4%) and high population's Q values (an average of 0.781) indicated that the level of genetic differentiation among populations is moderate. Outcrossing rate estimates based on eight microsatellite loci were 0.88 to 0.80 for two different populations, showing the mostly panmictic nature of the species. Substantial level of genetic variation within and among populations implicates that genetic improvement on desirable traits is possible via artificial selection. On the other hand, the present study showed that maintenance of population size is critical to avoid fitness decline in natural populations via inbreeding depression and fixation of deleterious alleles by genetic drift.

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“…Bands with strong intensity were recorded and classified as present (1) or absent (0) for all accessions to construct a binary data matrix, from which markers lower than 70 bp were excluded. Cluster analysis was carried out using the UPGMA method [11] with the NTSYSpc version 2.2 software package [18] based on Dice's coefficient. Factorial correspondence analysis (FCA) was carried out using GENETIX 4.05 software [19,20].…”

Section: Statistical Proceduresmentioning

confidence: 99%

“…However, genetic diversity and relationships among these two species and their hybrids are still in great need to be unveiled. Genetic diversity and cultivar discrimination studies of non-wood-product forests are currently supported by molecular methods, such as molecular makers and genome sequencing [11]. Amplified fragment length polymorphism (AFLP) technique is a reliable genetic marker with low cost, superior repeatability and high resolution [12].…”

Section: Introductionmentioning

confidence: 99%

“…Amplified fragment length polymorphism (AFLP) technique is a reliable genetic marker with low cost, superior repeatability and high resolution [12]. Thus, AFLP markers have been with broad application in systematics [13], genetic diversity [11,12,14], genetic linkage map construction [15] and association analysis [16] till now. Here, 35 clonal cultivars selected from the main cultivating area in China were applied in AFLP analysis.…”

Section: Introductionmentioning

confidence: 99%

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Molecular genetic variability of Juglans regia L. and Juglans sigillata D. as revealed by fluorescent amplified-fragment length polymorphism

Zhang

Pei

2020

Biotechnology & Biotechnological Equipment

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Juglans regia L. and J. sigillata D. have a long cultivation history in China. With the purpose of detecting interspecific and intraspecific genetic variation, 35 clonal cultivars of J. regia, J. sigillata, J. sigillataÂJ. regia and J. hopeiensis Hu. (J. mandshurica Maxim. ÂJ. regia) were selected from a broad geographic range and applied in amplified-fragment length polymorphism (AFLP) analysis. Clear-cut AFLP profiles were produced using 9 EcoR I/Mse I primer combinations. As for J. regia, J. sigillata and their hybrids, the percentages of polymorphic bands (PPBs) averaged 93.86%, 93.94% and 60.46%; the observed number of alleles (Na) averaged 1.5370, 1.6389 and 1.2593; the effective number of alleles (Ne) averaged 1.2582, 1.2543 and 1.1646; Nei's gene diversity (H) scored 0.1560, 0.1556 and 0.0951; Shannon's information index (I) averaged 0.2402, 0.2459 and 0.1414, respectively. These results implied a moderate level of genetic diversity within the investigated species. The total gene diversity (H t ¼ 0.1596) can be distributed into intra-(H s ¼ 0.1356) and inter-(D st ¼ 0.0240) species gene diversity. The proportion of the distributed genetic diversity among populations (G st ) was 0.1505, indicating that only 15% of the total gene diversity was attributed to differences between species. The factorial correspondence analysis (FCA) and unweighted pair-group method of arithmetic averages (UPGMA) analysis revealed a close genetic distance between these two species.

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Genetic diversity and population structure of endangeredGlehnia littoralis(Apiaceae) in China based on AFLP analysis

Cited by 16 publications

References 24 publications

Potential biological mechanisms underlying the endangered status of Glehnia littoralis revealed by nrDNA ITS and RAPD analyses

Potential biological mechanisms underlying the endangered status of Glehnia littoralis revealed by nrDNA ITS and RAPD analyses

Genetic diversity among Japanese local populations of an edible and medicinal coastal plant Glehnia littoralis F. Schmidt ex Miq.

Molecular genetic variability of Juglans regia L. and Juglans sigillata D. as revealed by fluorescent amplified-fragment length polymorphism

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