Genetic Diversity and Population Structure of Mycobacterium marinum: New Insights into Host and Environmental Specificities

Broutin, Vincent; Bañuls, Anne‐Laure; Aubry, Alexandra; Keck, Nicolas; Choisy, Marc; Bernardet, Jean‐François; Michel, Christian; Raymond, Jean-Christophe; Libert, Cédric; Barnaud, Antoine; Stragier, Pieter; Portaels, Françoise; Terru, Dominique; Belon, Claudine; Dereure, O.; Gutiérrez, Cristina; Boschiroli, Maria‐Laura; Perre, Philippe Van de; Cambau, Emmanuelle; Godreuil, Sylvain

doi:10.1128/jcm.01274-12

Cited by 18 publications

(10 citation statements)

References 34 publications

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“…Some authors have argued that the otomastoiditis attributed to M. fortuitum or M. chelonae was actually caused by M. abscessus [ 107 ], though this disagreement is actually a taxonomic issue. Different strains of the same species or subspecies of NTM have shown different degrees of virulence, with complex mechanisms of resistance against the host immune system [ 108–110 ]; strains may include some genotypes with a wide host spectrum, while others are avirulent [ 111 ]. Further research is needed to establish the genetic factors related to mycobacteria virulence [ 111 ].…”

Section: Species Distribution and Pathogenesismentioning

confidence: 99%

Nontuberculous mycobacterial osteomyelitis

et al. 2015

Infectious Diseases

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Osteomyelitis caused by nontuberculous mycobacteria (NTM) can have severe consequences and a poor prognosis. Physicians therefore need to be alert to this condition, especially in immunocompromised patients. Although the pathogenesis of NTM osteomyelitis is still unclear, studies in immunodeficient individuals have revealed close relationships between NTM osteomyelitis and defects associated with the interleukin-12–interferon-γ–tumor necrosis factor-α axis, as well as human immunodeficiency virus infection, various immunosuppressive conditions, and diabetes mellitus. Culture and species identification from tissue biopsies or surgical debridement tissue play crucial roles in diagnosing NTM osteomyelitis. Suitable imaging examinations are also important. Adequate surgical debridement and the choice of appropriate, combined antibiotics for long-term anti-mycobacterial chemotherapy, based on in vitro drug susceptibility tests, are the main therapies for these bone infections. Bacillus Calmette–Guerin vaccination might have limited prophylactic value. The use of multiple drugs and long duration of treatment mean that the therapeutic process needs to be monitored closely to detect potential side effects. Adequate duration of anti-mycobacterial chemotherapy together with regular monitoring with blood and imaging tests are key factors determining the recovery outcome in patients with NTM osteomyelitis.

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Section: Species Distribution and Pathogenesismentioning

confidence: 99%

Nontuberculous mycobacterial osteomyelitis

et al. 2015

Infectious Diseases

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“…The origin and other information concerning the tested M. marinum isolates are described in Broutin et al [ 5 ]. The 35 M. marinum isolates from human patients and nine from fish were collected in France between 1995 and 2007.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the SLOMYCO Sensititre® panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates

Chazel¹,

Marchandin²,

Keck³

et al. 2016

Ann Clin Microbiol Antimicrob

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BackgroundThe agar dilution method is currently considered as the reference method for Mycobacterium marinum drug susceptibility testing (DST). As it is time-consuming, alternative methods, such as the E-test, were evaluated for M.marinum DST, but without success. The SLOMYCO Sensititre® panel, recently commercialized by TREK Diagnostic Systems (Cleveland, OH), can be used for DST in slow-growing mycobacteria and for antimicrobial agents recommended by the Clinical and Laboratory Standards Institute (CLSI) for M.marinum DST. The main goal of this work was to evaluate the SLOMYCO Sensititre® panel method for DST in M.marinum isolates from human patients and fish relative to the reference agar dilution method.Methods/ResultsThe reproducibility of the minimum inhibitory concentration (MIC) determination (±1 log2 dilution) was very good for both the agar dilution method and SLOMYCO Sensititre® panel (>90 % agreement). The percentage essential agreement between methods varied, depending on the drug: between 97 and 75 % for ciprofloxacin, moxifloxacin, linezolid, isoniazid, clarithromycin, amikacin, rifabutin and rifampin, 74 % for trimethoprim, 72 % for doxycycline, 70 % for sulfamethoxazole, 59 % for streptomycin, 33 % for ethambutol and only 2.2 % for ethionamide. When the agar dilution and SLOMYCO Sensititre® panel results were converted into interpretive criteria, the category agreement was 100 % for amikacin, ciprofloxacin, clarithromycin, moxifloxacin, rifabutin, sulfamethoxazole and trimethoprim, 98 % for ethambutol and 96 % for rifampin and no agreement for doxycycline.ConclusionsThe SLOMYCO Sensititre® panel method could provide a potential alternative to the reference agar dilution method, when DST in M.marinum is required, except for doxycycline.

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“…marinum infections are primarily localised at the site of inoculation, typically in the upper extremities [32,80]. These infections are also known as "swimming pool granulomas" or "fish tank granulomas", since M. marinum is widespread in both fresh and marine water [84,85,32,33]. In 2008, an outbreak of M. marinum infections occurred on a fish farm in China that workers had contracted from abrasions, or trauma to the skin that had became infected.…”

Section: Atypical Mycobacterium Infections Of the Skinmentioning

confidence: 99%