Genetic diversity and population structure of sorghum [Sorghum bicolor (L.) Moench] genotypes in Ethiopia as revealed by microsatellite markers

Wubshet, Mamo; Enyew, Muluken; Mekonnen, Tilahun; Tesfaye, Kassahun; Feyissa, Tileye

doi:10.1016/j.heliyon.2023.e12830

Cited by 18 publications

(5 citation statements)

References 38 publications

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“…The moderate PIC value in this study was similar to that observed by Ali et al (2008) and Mangena et al (2018). However, higher polymorphic levels were reported by Tirfessa et al (2020) and Mamo et al (2023) in the study of sweet sorghum genotypes analysed with SSRs markers. The polymorphism of multiple genes controls the phenotypic diversity observed within a species (Buckler et al 2006).…”

Section: Resultssupporting

confidence: 88%

Estimation of genetic diversity of sweet sorghum (Sorghum bicolor (L.) Moench) genotypes as a bioethanol source using SSRs markers

Herniwati,

Pabendon,

Wicaksono

et al. 2024

Czech J. Genet. Plant Breed.

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Sweet sorghum is a cereal crop that can potentially serve as a source for bioethanol production. This study aims to analyse the genetic diversity of promising genotypes of sweet sorghum at the molecular level. The genetic material consisted of 12 sweet sorghum genotypes. The genetic diversity estimated using 59 SSRs markers showed a polymorphism value of 0.48 and the coefficient of genetic diversity was classified as moderate. The unweighted pair group method arithmetic average (UPGMA) analysis assigned the tested genotypes into three major clusters with a similarity coefficient level of 0.596. This indicates that the genetic similarity of the tested genotypes is moderate to high. Eight unique loci were identified with the SSRs markers in six genotypes, which are considered to control high sugar traits.

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Section: Resultssupporting

confidence: 88%

Estimation of genetic diversity of sweet sorghum (Sorghum bicolor (L.) Moench) genotypes as a bioethanol source using SSRs markers

Herniwati,

Pabendon,

Wicaksono

et al. 2024

Czech J. Genet. Plant Breed.

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“…They can be also sources of parental lines for further breeding activities. Our finding confirms that the Ethiopian sorghum landraces often harbored various important traits explained by earlier researchers [2,5,22,24].…”

Section: Discussionsupporting

confidence: 91%

“…Ethiopian farmers grow diverse forms of sorghum varieties with over 95% of the area allocated for sorghum production covered by landraces [16] Landraces are heterogeneous and adapted to diverse growing conditions and are potential sources of desirable traits for crop improvement [17][18]. Given its center of origin and diversity [19][20], and tremendous variation in agro-ecologies, Ethiopia is harbored with extremely rich genetic diversity of sorghum landraces [2,16,[21][22], which serves as sources of various important traits. For instance, drought tolerance [16,[23][24], nutritional quality traits [21,[25][26], resistance to grain mold [27] and resistant to ergot and green bug [28].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity, Correlation and Genotype by Yield by Trait (GYT) Analysis of Grain Yield and Nutritional Quality Traits in Sorghum (Sorghum bicolor [L.] Moench) Genotypes in Tigray, northern Ethiopia

Welderufael,

Abay,

Ayana

et al. 2023

Preprint

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Selecting sorghum genotypes with higher grain yield and nutritional quality is essential to tackle food insecurity and malnutrition in arid and semi-arid areas. Therefore, this study aimed to determine the genetic diversity, trait association and genotype by yield by trait (GYT) analysis and to select superior sorghum genotypes. One hundred and ten sorghum genotypes were evaluated at three locations in Tigray during the 2018 and 2019 growing seasons using alpha lattice design. Traits such as grain yield, protein content, ash content, starch content, zinc content, iron content, calcium content and magnesium content were profiled. Results showed that wide range and highly significant (p < 0.001) genotype mean performance in each environment as well as combined environments. Several highly performing genotypes were distinguished for each trait studied that could be exploited as breeding parents or direct use. This study further detected highly significant variation (p < 0.001) among the test genotypes for all the traits studied in individual environments and across environments suggesting the presence of sufficient genetic diversity for selection. The high broad-sense heritability (H2 > 0.9) in all individual environments and moderate to high (H2 > 0.0.41 < 0.82) in pooled environments recorded in the present study assured the possibility of effective selection among the genotypes. Besides, strong positive and negative associations were detected between some of the traits in individual and across environments. The significant positive association between traits indicates that both the traits can be improved concurrently through direct selection. Using the GYT analysis, we suggest ten promising sorghum genotypes for direct use or breeding programs in arid and semi-arid areas in general and in Tigray in particular.

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“…The bands appeared on the gels were scored as A, B, H, OFF and "-" based on their pattern compared with those of the parents. "A" is referred to as the presence of allele from the recurrent parent GS-23, "B" is referred to as the presence of allele from K260 and K359w, "H" is referred as the heterozygous (i.e., presence of both recurrent and donor parent alleles), "OFF" was defined as an allele neither from donor parent nor from the recurrent parent and "-" is referred as a missing sample [8].…”

Section: Scanning and Scoring Of The Gelsmentioning

confidence: 99%

Introgression of Stay Green Quantitative Trait Locus (QTLS) into Elite Sorghum Variety by MABC

Priyanka,

Girish,

Lokesha

et al. 2023

IJECC

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Sorghum is a major staple food crop for the people in semi-arid areas of Asia. Post-flowering drought is a global constraint of sorghum production. The study aimed to improve stay-green characteristics of GS-23 sorghum variety by transferring stg3A and stg3B QTL`s respectively from donor genotypes K260 and K359w of ICRISAT by using marker assisted backcrossing. The experimental material comprised of six basic generations P1, P2, F1, F2, BC1 and BC2 developed from crossing GS-23 × K260 and GS-23 × K359 were genotyped using a set of 133 SNPs and 79 SSR markers. Whereas, 53 polymorphic SNPs among parents and backcross F1s for stay green trait at maturity were used to track introgression of stay green trait. Similarly,10 SSR markers were found to be polymorphic were used to track introgression of stay green trait i.e., stay green trait QTL`s stg3A and stg3B from donor parent K260 and K359w respectively in GS-23 background across backcross population and to identify plants that were homozygous for the desired allele. In which 02 SNPs SnpSB0039 and SnpSB0093 were identified as polymorphic for both K260 and K359w. Whereas 17 SNPs identified polymorphic for K260 and 34 SNPs for K359w. These SNPs were validated in both F1 and BC1F1 populations of both the crosses. Similarly, out of 10 SSR markers utilized, 02 SSRs were identified polymorphic to K260 and 02 SSRs were identified for K359w. Whereas 06 SSRs were polymorphic to both the parents K260 and K359w. Prominent 02 SSRs viz., Xtxp 141_Fam and Xgap84_Vic found to be more reliable and polymorphic to both the parents. These SSRs were validated in both F1 and BC1F1 populations of both the crosses. The genotypic analysis revealed the presence of favorable alleles in homozygous conditions at markers loci associated with stg3A and stg3B QTL`s in BC populations, suggesting successful introgression of stay green QTLs from the donor parents to the recurrent parent. Therefore, our study demonstrated the utility of marker-assisted backcrossing for drought tolerance improvement of locally adapted sorghum variety.

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Genetic diversity and population structure of sorghum [Sorghum bicolor (L.) Moench] genotypes in Ethiopia as revealed by microsatellite markers

Cited by 18 publications

References 38 publications

Estimation of genetic diversity of sweet sorghum (Sorghum bicolor (L.) Moench) genotypes as a bioethanol source using SSRs markers

Estimation of genetic diversity of sweet sorghum (Sorghum bicolor (L.) Moench) genotypes as a bioethanol source using SSRs markers

Genetic Diversity, Correlation and Genotype by Yield by Trait (GYT) Analysis of Grain Yield and Nutritional Quality Traits in Sorghum (Sorghum bicolor [L.] Moench) Genotypes in Tigray, northern Ethiopia

Introgression of Stay Green Quantitative Trait Locus (QTLS) into Elite Sorghum Variety by MABC

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