Genetic diversity and population structure of castor (Ricinus communis L.) germplasm within the US collection assessed with EST-SSR markers

Abstract

Genetic diversity and population structure of castor (Ricinus communis L.) germplasm within the U.S. collection assessed with EST-SSR markers AbstractCastor is an important oilseed crop and although its oil is inedible, it has multiple industrial and pharmaceutical applications. The entire U.S. castor germplasm collection was previously screened for oil content and fatty acid composition, but its genetic diversity and population structure has not been determined. Based on the screening results of oil content, fatty acid composition, and country origins, 574 accessions were selected and genotyped with 22 polymorphic EST-SSR markers. The results from cluster analysis, population structure, and principal component analysis were consistent, and partitioned accessions into four subpopulations. Although there were certain levels of admixtures among groups, these clusters and subpopulations aligned with geographic origins. Both divergent and redundant accessions were identified in this study. The U.S. castor germplasm collection encompasses a moderately high level of genetic diversity (pairwise dissimilarity coefficient = 0.53). The results obtained here will be useful for choosing accessions as parents to make crosses in breeding programs and prioritizing accessions for regeneration to improve germplasm management. A subset of 230 accessions was selected and will be planted in the field for establishing a core collection of the U.S. castor germplasm. Further evaluation of the U.S. castor germplasm collection is also discussed.

Genetic diversity analysis among 123 accessions of castor (Ricinus communis L.) using SSR marker and its association to agronomic traits

“…The result demonstrated that castor collections originating from a variety of Indonesian islands have moderate genetic diversity (as indicated by the He/Gene diversity score of 0.5). Senthilvel et al (2016) and Wang et al (2017) reported a modest degree of genetic diversity in castor collections. On the other hand, Seo et al (2011) found a low He value (0.30) among Korean castor accessions.…”

“…The marker is highly informative, codominant, multi-allele, reproducible, and transferable among related species (Vieira et al 2016). Some studies on genetic diversity and agronomic trait analysis have been conducted on castor, such as Wang et al (2017), Wahibah et al (2020), Dharajiya et al (2020), and Anggraeni and Purwati (2022).…”

Morphological, Genetic Characterization, and Chemical Analysis of Castor Bean ( Ricinus communis ) Growing in Riyadh Saudi Arabia

“…574 accessions were chosen and genotyped with 22 polymorphic EST-SSR markers based on the screening results for oil content, fatty acid composition, and country of origin. Cluster analysis, population structure, and principal component analysis all produced consistent results, dividing accessions into four subpopulations [30]. Polymorphism was found in EST-SSRs.…”

Phylogenetics and diversity analysis of Pennisetum species using Hemarthria EST‐SSR markers

“…For the 35 accessions, all of the 21 EST‐SSR makers had PIC > 0.5, indicating that they could provide reliable information to the analysis of the relationship of the 35 accessions. Due to the high polymorphism and efficiency of EST‐SSR makers, they have been widely used for genetic mapping (Ferrão et al., ), evaluating genetic diversity (Wang et al., ), phylogenetic analysis (Wang et al., ), and many other research fields.…”