Genetic Diversity and Quinolone Resistance in
 Campylobacter jejuni
 Isolates from Poultry in Senegal

Kinana, Alfred; Cardinale, Éric; Tall, F.; Bahsoun, Ibrahim; Sire, Jean-Marie; Garin, Benoît; Breurec, Sébastien; Boye, Cheikh Saad-Bouh; Perrier-Gros-Claude, Jean-David

doi:10.1128/aem.72.5.3309-3313.2006

Cited by 54 publications

(45 citation statements)

References 26 publications

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“…These results suggest that the emergence of resistance among C. jejuni isolates reflects antimicrobial selection pressure among isolates from different lineages rather than the diffusion of a unique clone. In their studies of C. jejuni chicken isolates collected in Senegal, Kinana et al similarly observed that quinolone resistance was variable within STs (20). Of interest, they also noted the highest rate of quinolone resistance among isolates in CC 353.…”

Section: Discussionmentioning

confidence: 82%

Multilocus Sequence Typing of Campylobacter jejuni Isolates from Humans, Chickens, Raw Milk, and Environmental Water in Quebec, Canada

et al. 2008

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Molecular strain typing is essential for deciphering the epidemiology of Campylobacter jejuni infections. We applied two different methods, multilocus sequence typing (MLST) and analysis of the flaA short variable repeat (SVR), to 289 isolates (163 human, 56 chicken, 34 raw milk, and 36 environmental water isolates) collected in the province of Québec, Canada, over 3 years; in addition, the analysis included the pulsed-field gel electrophoresis (PFGE) typing results for a subset of 131 isolates studied previously. MLST defined 96 sequence types (STs) and 20 clonal complexes (CCs), including 49 STs (73 isolates, 25%) and 39 alleles not previously documented in an international database. The frequency of new STs was significantly higher among water isolates than among isolates from other sources (18/36 [50%] and 55/253 [22%], respectively; P < 0.001). Nine of the 10 most prevalent CCs included isolates from humans and at least one other source; five CCs comprised exclusively or mostly human and chicken isolates. However, water and milk were the predominant nonhuman sources among the remaining CCs, suggesting that sporadic C. jejuni infections in humans may frequently arise from sources other than chickens. All three typing systems were discriminatory (discriminatory index > 0.9). Among 131 isolates analyzed by PFGE, each of the 20 types represented by two or more isolates corresponded to a single CC. In contrast, among the 14 most prevalent types detected by analysis of the flaA SVR (5 to 27 isolates each), 8 (57%) included isolates that represented multiple different CCs. The basis for these discordant results was uncertain. Antimicrobial resistance was randomly distributed among the CCs and appeared to be more closely related to the source of an isolate than its genotype. Although MLST is labor-intensive and expensive, it remains the single best method for the genotyping of C. jejuni isolates and deciphering the epidemiologic relationships among isolates.

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Section: Discussionmentioning

confidence: 82%

Multilocus Sequence Typing of Campylobacter jejuni Isolates from Humans, Chickens, Raw Milk, and Environmental Water in Quebec, Canada

et al. 2008

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“…The degree of association between specific genotypes and drug resistance in Campylobacter and Salmonella strains remains a topic of debate. Some researchers reported that drug resistance is associated with specific genotypes (25)(26)(27)(28)(29), whereas others reported no such associations (30,31). Because the number of isolates analyzed in this study was limited, further studies should be conducted to elucidate the association between genotypes and drug resistance in Campylobacter more precisely.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Characteristics of Salmonella and Campylobacter in Retail Poultry Meat in Japan

et al. 2017

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“…This requires the development of reference data sets representing the host-and environmentassociated populations from which human infections originate (9,11,16,21,23,24,26,28,29,31,37,41). An understanding of the population biology of host-associated Campylobacter populations (29,38), including phylogeographic effects (29), is needed to use them effectively.…”

Section: Discussionmentioning

confidence: 99%

“…In both analyses, this comprised 742 isolates from the United Kingdom retail poultry survey described here, 590 from United Kingdom ruminants (cattle and sheep), 160 from United Kingdom wild birds, and 91 from United Kingdom environmental samples. The details of the samples from published sources are given in the original studies (10,16,24,28,29,37). For C. coli, three comparisons were performed.…”

Section: Methodsmentioning

confidence: 99%

Host Association of Campylobacter Genotypes Transcends Geographic Variation

Sheppard

Colles

Richardson³

et al. 2010

Appl Environ Microbiol

126

142

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Genetic attribution of bacterial genotypes has become a major tool in the investigation of the epidemiology of campylobacteriosis and has implicated retail chicken meat as the major source of human infection in several countries. To investigate the robustness of this approach to the provenance of the reference data sets used, a collection of 742 Campylobacter jejuni and 261 Campylobacter coli isolates obtained from United Kingdomsourced chicken meat was established and typed by multilocus sequence typing. Comparative analyses of the data with those from other isolates sourced from a variety of host animals and countries were undertaken by genetic attribution, genealogical, and population genetic approaches. The genotypes from the United Kingdom data set were highly diverse, yet structured into sequence types, clonal complexes, and genealogical groups very similar to those seen in chicken isolates from the Netherlands, the United States, and Senegal, but more distinct from isolates obtained from ruminant, swine, and wild bird sources. Assignment analyses consistently grouped isolates from different host animal sources regardless of geographical source; these associations were more robust than geographic associations across isolates from three continents. We conclude that, notwithstanding the high diversity of these pathogens, there is a strong signal of association of multilocus genotypes with particular hosts, which is greater than the geographic signal. These findings are consistent with local and international transmission of host-associated lineages among food animal species and provide a foundation for further improvements in genetic attribution.Members of the genus Campylobacter, specifically Campylobacter jejuni and Campylobacter coli, are major causes of human morbidity worldwide and are the most common bacterial cause of gastroenteritis in industrialized countries (4). These bacteria are commonly found as apparently harmless members of the gut microbiota of many farmed and wild mammals and birds. This, together with the sporadic nature of most human disease, has contributed to the remaining uncertainty regarding the relative importance of different potential sources of human infection (34), inhibiting the implementation of effective public health interventions, which may have major economic consequences on intensive food production. Human infection with C. jejuni and C. coli has been epidemiologically linked to contact with pets and farm animals and to consumption of red meat, water, milk, and poultry (17,20,34).The advent of multilocus sequence typing (MLST) for both C. jejuni and C. coli and its application to large and diverse isolate collections have enhanced understanding of the ecology (38) and epidemiology (10) of these important pathogens. It has been shown that there is substantial genetic differentiation between farmed ruminants and chickens (29) and even greater differentiation between farmed chickens and wild birds at the same farm site (5). Furthermore, MLST supports the application of popul...

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Genetic Diversity and Quinolone Resistance in Campylobacter jejuni Isolates from Poultry in Senegal

Cited by 54 publications

References 26 publications

Multilocus Sequence Typing of Campylobacter jejuni Isolates from Humans, Chickens, Raw Milk, and Environmental Water in Quebec, Canada

Multilocus Sequence Typing of Campylobacter jejuni Isolates from Humans, Chickens, Raw Milk, and Environmental Water in Quebec, Canada

Prevalence and Characteristics of <i>Salmonella</i> and <i>Campylobacter</i> in Retail Poultry Meat in Japan

Host Association of Campylobacter Genotypes Transcends Geographic Variation

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