Genetic Diversity and Recombination of Porcine Sapoviruses

Wang, Qiuhong; Han, Myung Guk; Funk, Julie A.; Bowman, Gary; Janies, Daniel; Saif, Linda J.

doi:10.1128/jcm.43.12.5963-5972.2005

Cited by 75 publications

(115 citation statements)

References 33 publications

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“…Porcine SaV QW270 was a potential recombinant strain between a JJ259-like strain (RdRp region) and a Cowden-like strain (capsid, after RdRp) of the same genotype in SaV genogroup III [50]. A recombination event probably occurred for porcine SaV MM280 and a Cowden-like strain, although a strain carrying a similar RdRp but different capsid from that of the MM280 strain was not detected.…”

Section: Recombination Of Porcine Novs and Savsmentioning

confidence: 98%

“…The full name of a NoV or SaV strain is organized as follows: Host species from which the virus was obtained/ genus/genogroup-genotype/strain name/year of occurance/country of origin [1]. The classification and GenBank accession numbers of representative NoV and SaV strains were summarized in two published papers [15,50].…”

Section: Genetic Organization and Classification Of Novs And Savsmentioning

confidence: 99%

“…Presently, porcine SaVs have been reported from the US, Venezuela, South Korea and Japan [44,45,50,54,55]. Most strains are Cowden-like (GIII) SaVs based on sequence in the capsid or RdRp region except for QW19/02/US, JJ681/00/US, LL26/02/US and K7/JP strains.…”

Section: Genetic Classification Of Porcine Savsmentioning

confidence: 99%

“…Most strains are Cowden-like (GIII) SaVs based on sequence in the capsid or RdRp region except for QW19/02/US, JJ681/00/US, LL26/02/US and K7/JP strains. Interestingly, the QW19 strain shared the highest amino acid identity (66.3%) with human GII SaVs Mc10, C12 and Cruise ship, but not with any porcine SaV strains, in a sequence fragment of 95 amino acid (aa) in the RdRp region [50]. Although it is impossible to accurately classify the QW19 strain as a certain genotype based only on the 95 aa RdRp sequence, it likely represents a new genogroup because the overall intragenogroup amino acid identity of the 95 aa RdRp region of SaVs is 80-100% [50].…”

Section: Genetic Classification Of Porcine Savsmentioning

confidence: 99%

“…Although it is impossible to accurately classify the QW19 strain as a certain genotype based only on the 95 aa RdRp sequence, it likely represents a new genogroup because the overall intragenogroup amino acid identity of the 95 aa RdRp region of SaVs is 80-100% [50]. The complete capsid protein of JJ681 strain has been sequenced [50]. It shares only 32-36% identity to each SaV genogroup (GI-GV); thus, it represents a new SaV genogroup (GVI?).…”

Section: Genetic Classification Of Porcine Savsmentioning

confidence: 99%

See 4 more Smart Citations

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Wang

Costantini

Saif

2007

Vaccine

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Porcine enteric caliciviruses include sapoviruses and noroviruses. Porcine sapoviruses infect pigs of all ages and cause diarrhea in young pigs, whereas porcine noroviruses were detected exclusively from adult pigs without clinical signs. Importantly, certain porcine norovirus strains were genetically and antigenically related to human noroviruses. This raises public health concerns that pigs may be reservoirs for emergence of epidemic human norovirus strains. This article reviews the discovery of porcine noroviruses and sapoviruses, their classification, diagnosis, epidemiology and genetic and antigenic relatedness to human caliciviruses.

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Section: Recombination Of Porcine Novs and Savsmentioning

confidence: 98%

Section: Genetic Organization and Classification Of Novs And Savsmentioning

confidence: 99%

Section: Genetic Classification Of Porcine Savsmentioning

confidence: 99%

Section: Genetic Classification Of Porcine Savsmentioning

confidence: 99%

Section: Genetic Classification Of Porcine Savsmentioning

confidence: 99%

See 3 more Smart Citations

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Wang

Costantini

Saif

2007

Vaccine

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Caliciviruses

Knowles¹,

Reuter²

2019

Diseases of Swine

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Detection of human sapovirus by real‐time reverse transcription‐polymerase chain reaction

Oka

Katayama

Hansman

et al. 2006

Journal of Medical Virology

237

183

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Sapovirus (SaV) is an agent of gastroenteritis for humans and swine, and is divided into five distinct genogroups (GI-GV) based on its capsid gene sequences. Typical methods of SaV detection include electron microscopy (EM), enzyme-linked immunosorbent assay (ELISA), and reverse transcription-polymerase chain reaction (RT-PCR). A novel TaqMan-based real-time RT-PCR assay was developed that is sensitive and has the ability to detect the broad range of genetically diverse human SaV strains. A nucleotide alignment of 10 full-length SaV genome sequences was subjected to similarity plot analysis, which indicated that the most conserved site was the polymerase-capsid junction in open reading frame 1 (ORF1). Based on multiple alignments of the 27 available sequences encoding this junction, we designed sets of primers and TaqMan MGB probes that detect human SaV GI, GII, GIV, and GV sequences in a single tube. The reactivity was confirmed with SaV GI, GII, GIV, and GV control plasmids, and the efficiency ranged from 2.5 x 10(7) to 2.5 x 10(1) copies per tube. Analysis using clinical stool specimens revealed that the present system was capable of detecting SaV GI, GII, GIV, and GV sequences, and no cross-reactivity was observed against other enteric viruses, including norovirus (NoV), rotavirus, astrovirus, and adenovirus. This is the first real-time RT-PCR system that could detect all genogroups of human sapoviruses.

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Genetic Diversity and Recombination of Porcine Sapoviruses

Cited by 75 publications

References 33 publications

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Caliciviruses

Detection of human sapovirus by real‐time reverse transcription‐polymerase chain reaction

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