Genetic Diversity and Relationships Among Some Barley Genotypes for Net Blotch Disease Resistance Using Rapd, Scot and SSR Markers

Dora, S. A.; Mansour, Mohamed; Aboulila, Aziza A.; Abdel-Wahab, Eman Ibrahim

doi:10.21608/ejgc.2018.9510

Cited by 7 publications

(9 citation statements)

References 25 publications

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“…These values were lower than those previously reported on the basis of SSRs (60.5 to 93.9%) between Egyptian and ICARDA barley genotypes [18]. This is probably because of unequal distribution of SSR motifs throughout the genome in the form of clusters could not represent the entire genome well [23].…”

Section: Relationships Among Barley Genotypescontrasting

confidence: 77%

“…Similar anomalies in cluster analysis were also observed [18] who examined genomic and expressed molecular diversity of barley genotypes through SCoT and SSR analysis. Thus, phylogenetic studies revealed tight groupings among the barley genotypes with some incongruities in position of genotypes.…”

Section: Relationships Among Barley Genotypessupporting

confidence: 69%

“…A total of 7 microsatellite specific primer pairs (SSR-1 to SSR-7) (iNtRON Biotechnology, Inc, Korea) ( Table 1) were tested. PCR reaction was performed as previously described [18].…”

Section: Microsatellite (Ssr) Analysismentioning

confidence: 99%

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Efficiency of Triple-SCoT Primer in Characterization of Genetic Diversity and Genotype-Specific Markers against SSR Fingerprint in Some Egyptian Barley Genotypes

Aboulila¹,

Mansour²

2017

AJMB

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Ten Egyptian barley genotypes (2 commercial varieties and 8 breeding lines) were cultivated under normal condition at the Experimental Farm of Sakha Agricultural Research station and exposed to salinity stress condition at the Experimental Farm of El-hosainia plain Agricultural Research station, Elsharkia Governorate, Egypt, in an attempt to identify the relative salinity tolerant genotypes. A susceptibility index (SI) was used to estimate the relative stress loss because it accounted for variation in yield potential and stress intensity. Giza 123, Line-1, Line-5, Line-6 and Line-8 genotypes were considered as saline tolerant genotypes on the basis of their highly tolerance indices values. Barley genotypes were characterized by seven SSR markers and three SCoT primers in different combinations to discern the extent of genetic variation and develop a fingerprinting key. Normal SCoT reactions amplify single segments of DNA which are 15-to 19-mer long. A new strategy was used to increase SCoT potential in genetic diversity studies by using two and three different primer combinations per reaction. Amplification products scored a polymorphism percentage of 94.44% for Triple-SCoT and 90.91% for SSR, while the average no. of polymorphic fragments/primer was 17 and 7.14 in the two marker systems, respectively. On the other side, Triple-SCoT exhibited the highest average number of positive and negative genotype-specific markers. The cluster analysis of the studied genotypes using these different marker systems revealed four dendrograms varied in their topology. The dendrogram based on Triple-SCoT data exhibited the closest relationships to those illustrated by SSR dendrogram.

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Section: Relationships Among Barley Genotypescontrasting

confidence: 77%

Section: Relationships Among Barley Genotypessupporting

confidence: 69%

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Efficiency of Triple-SCoT Primer in Characterization of Genetic Diversity and Genotype-Specific Markers against SSR Fingerprint in Some Egyptian Barley Genotypes

Aboulila¹,

Mansour²

2017

AJMB

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“…Moreover, Gorji et al (2011) presented that SCoT markers were more informative and compelling, followed by ISSRs and AFLP markers in fingerprinting of potato varieties. However, the simplicity and reproducibility of SCoT have been successfully applied to the assessment of genetic diversity and taxonomic study of Citrus (Han et al 2011); rice (Collard and Mackill 2009); and barley (Aboulila and Mansour, 2017;Dora et al 2017).…”

Section: Scot Markers For Drought Tolerancementioning

confidence: 99%

Genetic improvement for drought tolerance in rice using mutation induction

et al. 2022

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Thirty-three percent of the world's farmland is subject to drought, making it the most difficult abiotic stress on rice production. Ten different M4-rice mutants were tested, along with three check varieties (Giza 179, Sakha 107, and IET1444 - International check variety for drought stress), to see how well they fared in drought conditions. These genotypes were tested in well-watered (WW: irrigation every 4 days), water-stressed (WS1: irrigation every 8 days), and severe water-stressed (WS2: irrigation every 12 days) conditions across generations M5 to M8. Drought stress was measured regarding its effect on agronomic traits and drought tolerance indices. Of the ten tested mutants, seven high-tillering mutants had higher yields under normal and stress conditions than the check varieties did in the field. The STI, MP, YI, and GMP indices show that, compared to IET444 (DT check variety), the mutant EN25 performed best under drought stress, followed by the mutant EN27. According to the data analysis of SCoT markers, only 34 of the 46 primers used amplified 377 bands (alleles) across 53 different markers. There was a wide range of genetic similarities among mutants, parents, and the check varieties, and it ranged from 17% to 78%. These seven mutants shared 13 common bands with the most drought-tolerant check variety (IET444) using SCoT markers, which indicates that these mutants carried some drought-tolerant genes. Hence, these mutants hold great potential for use in drought-stressed rice breeding programs.

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“…This method provides high polymorphism and reproducible markers related with characters of biological interest (Collard & Mackill 2009). SCoT marker system has been successfully applied in various crops such as Tunisian citrus species (Mahjbi et al 2015), landrace chickpea (Pakseresht et al 2013), durum wheat (Etminan et al 2016, cultivars of Egyptian wheat (Abdel-Lateif & Hewedy 2018), and 20 barley genotypes (Dora et al 2017). Retrotransposons are repetitive and mobile sequences and observed in virtually all known eukaryotic genomes (Flavell et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers

Güngör¹,

İlhan²,

KASAPOĞLU³

et al. 2021

Tarım Bilimleri Dergisi

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Genetic Diversity and Relationships Among Some Barley Genotypes for Net Blotch Disease Resistance Using Rapd, Scot and SSR Markers

Cited by 7 publications

References 25 publications

Efficiency of Triple-SCoT Primer in Characterization of Genetic Diversity and Genotype-Specific Markers against SSR Fingerprint in Some Egyptian Barley Genotypes

Efficiency of Triple-SCoT Primer in Characterization of Genetic Diversity and Genotype-Specific Markers against SSR Fingerprint in Some Egyptian Barley Genotypes

Genetic improvement for drought tolerance in rice using mutation induction

Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers

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