Genetic diversity and spatial genetic structure of Pinus strobus (Pinaceae) across an island landscape inferred from allozyme and cpDNA markers

Myers, E. R.; Chung, Mi Yoon

doi:10.1007/s00606-006-0498-6

Cited by 10 publications

(10 citation statements)

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“…This is the first report of chloroplast microsatellite genetic diversity across the range in eastern white pine. The haplotype diversity observed in our study is lower than that reported for four eastern white pine populations sampled from the Beaver Island Archipelago in Michigan (H = 0.80) [ 52 ]. The differences are likely due to the differences in the sample size and the number of cpSSRs used between the two studies.…”

Section: Discussioncontrasting

confidence: 92%

“…This is consistent with the lower mutation rate in chloroplast than nuclear microsatellites in Pinus [ 50 ] and other plants. Chloroplast microsatellites (cpSSR) have been previously used to test the somatic stability of the cloned material [ 51 ] and spatial genetic structure [ 52 ] in eastern white pine. This is the first report of chloroplast microsatellite genetic diversity across the range in eastern white pine.…”

Section: Discussionmentioning

confidence: 99%

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Post-glacial phylogeography and evolution of a wide-ranging highly-exploited keystone forest tree, eastern white pine (Pinus strobus) in North America: single refugium, multiple routes

Zinck¹,

Rajora²

2016

BMC Evol Biol

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BackgroundKnowledge of the historical distribution and postglacial phylogeography and evolution of a species is important to better understand its current distribution and population structure and potential fate in the future, especially under climate change conditions, and conservation of its genetic resources. We have addressed this issue in a wide-ranging and heavily exploited keystone forest tree species of eastern North America, eastern white pine (Pinus strobus). We examined the range-wide population genetic structure, tested various hypothetical population history and evolutionary scenarios and inferred the location of glacial refugium and post-glacial recolonization routes. Our hypothesis was that eastern white pine survived in a single glacial refugium and expanded through multiple post-glacial recolonization routes.ResultsWe studied the range-wide genetic diversity and population structure of 33 eastern white pine populations using 12 nuclear and 3 chloroplast microsatellite DNA markers. We used Approximate Bayesian Computation approach to test various evolutionary scenarios. We observed high levels of genetic diversity, and significant genetic differentiation (FST = 0.104) and population structure among eastern white pine populations across its range. A south to north trend of declining genetic diversity existed, consistent with repeated founder effects during post-glaciation migration northwards. We observed broad consensus from nuclear and chloroplast genetic markers supporting the presence of two main post-glacial recolonization routes that originated from a single southern refugium in the mid-Atlantic plain. One route gave rise to populations at the western margin of the species’ range in Minnesota and western Ontario. The second route gave rise to central-eastern populations, which branched into two subgroups: central and eastern. We observed minimal sharing of chloroplast haplotypes between recolonization routes but there was evidence of admixture between the western and west-central populations.ConclusionsOur study reveals a single southern refugium, two recolonization routes and three genetically distinguishable lineages in eastern white pine that we suggest to be treated as separate Evolutionarily Significant Units. Like many wide-ranging North American species, eastern white pine retains the genetic signatures of post-glacial recolonization and evolution, and its contemporary population genetic structure reflects not just the modern distribution and effects of heavy exploitation but also routes northward from its glacial refugium.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-016-0624-1) contains supplementary material, which is available to authorized users.

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Section: Discussioncontrasting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Post-glacial phylogeography and evolution of a wide-ranging highly-exploited keystone forest tree, eastern white pine (Pinus strobus) in North America: single refugium, multiple routes

Zinck¹,

Rajora²

2016

BMC Evol Biol

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“…So far, relevant studies have focused on the species’ native distribution range and have dealt with population genetic structure [100]–[105] or spatial genetic structure [47], [68], [69], [106], [107]. The main findings of the present study are:…”

Section: Discussionmentioning

confidence: 84%

European Invasion of North American Pinus strobus at Large and Fine Scales: High Genetic Diversity and Fine-Scale Genetic Clustering over Time in the Adventive Range

et al. 2013

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BackgroundNorth American Pinus strobus is a highly invasive tree species in Central Europe. Using ten polymorphic microsatellite loci we compared various aspects of the large-scale genetic diversity of individuals from 30 sites in the native distribution range with those from 30 sites in the European adventive distribution range. To investigate the ascertained pattern of genetic diversity of this intercontinental comparison further, we surveyed fine-scale genetic diversity patterns and changes over time within four highly invasive populations in the adventive range.ResultsOur data show that at the large scale the genetic diversity found within the relatively small adventive range in Central Europe, surprisingly, equals the diversity found within the sampled area in the native range, which is about thirty times larger. Bayesian assignment grouped individuals into two genetic clusters separating North American native populations from the European, non-native populations, without any strong genetic structure shown over either range. In the case of the fine scale, our comparison of genetic diversity parameters among the localities and age classes yielded no evidence of genetic diversity increase over time. We found that SGS differed across age classes within the populations under study. Old trees in general completely lacked any SGS, which increased over time and reached its maximum in the sapling stage.ConclusionsBased on (1) the absence of difference in genetic diversity between the native and adventive ranges, together with the lack of structure in the native range, and (2) the lack of any evidence of any temporal increase in genetic diversity at four highly invasive populations in the adventive range, we conclude that population amalgamation probably first happened in the native range, prior to introduction. In such case, there would have been no need for multiple introductions from previously isolated populations, but only several introductions from genetically diverse populations.

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“…A possible explanation for this phenomenon is that the cpDNA genome behaves as a single locus and the spatial autocorrelation of chloroplast haplotypes is subject to higher stochasticity than the multi-locus nSSRs. Thus, drift may have a stronger effect on the pattern of genetic differentiation of the chloroplast haplotypes than on that measured by nuclear markers (Myers et al 2007). Nevertheless, we cannot completely rule out that partly low sample sizes contributed to the overall variance in values of genetic differentiation.…”

Section: Population Genetic Comparison Of Nssr and Cpssr Markersmentioning

confidence: 84%

“…Thus, chloroplastic loci are prone to higher stochasticity and have higher susceptibility to genetic drift than nuclear loci (Birky et al 1989;Petit et al 1993;Ennos 1994;Ennos 1997, 1999;Ribeiro et al 2002). Consequently, population genetic differentiation is expected to be higher for chloroplast loci than for nuclear genes, and their spatial autocorrelation will be a better indicator for genetic drift (Myers et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic structure in Pinus cembra from the Carpathian Mountains inferred from nuclear and chloroplast microsatellites confirms post-glacial range contraction and identifies introduced individuals

Lendvay

Höhn

Brodbeck

et al. 2014

Tree Genetics & Genomes

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Genetic differentiation of scattered populations at neutral loci is characterized by genetic drift counteracted by the remaining gene flow. Populations of Pinus cembra in the Carpathian Mountains are isolated and restricted to island-like stands at high-elevation mountain ranges. In contrast, paleobotanical data suggest an extended early Holocene distribution of P. cembra in the Carpathians and its surrounding areas, which has contracted to the currently disjunct occurrences. We analyzed the genetic variation of 11 Carpathian populations of P. cembra at chloroplast and, in part newly developed, nuclear microsatellites. Both marker types revealed low levels of genetic differentiation and a lack of isolation by distance, reflecting the post-glacial retraction of the species to its current distribution. Stronger effects of genetic drift were implied by the higher genetic differentiation found for haploid chloroplast than for diploid nuclear markers. Moreover, we found no association between the values of population genetic differentiation for the two marker types. Several populations indicated recent genetic bottlenecks and inbreeding as a consequence of decline in population sizes. Moreover, we found individuals in two populations from the Rodnei Mountains that strikingly differed in assignment probabilities from the remaining specimens, suggesting that they had been introduced from a provenance outside the studied populations. Comparison with Eastern Alpine P. cembra and individuals of the closely related Pinus sibirica suggests that these individuals presumably are P. sibirica. Our study highlights the importance of the maintenance of sufficiently large local population sizes for conservation due to low connectivity between local occurrences.

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Genetic diversity and spatial genetic structure of Pinus strobus (Pinaceae) across an island landscape inferred from allozyme and cpDNA markers

Cited by 10 publications

References 46 publications

Post-glacial phylogeography and evolution of a wide-ranging highly-exploited keystone forest tree, eastern white pine (Pinus strobus) in North America: single refugium, multiple routes

Post-glacial phylogeography and evolution of a wide-ranging highly-exploited keystone forest tree, eastern white pine (Pinus strobus) in North America: single refugium, multiple routes

European Invasion of North American Pinus strobus at Large and Fine Scales: High Genetic Diversity and Fine-Scale Genetic Clustering over Time in the Adventive Range

Genetic structure in Pinus cembra from the Carpathian Mountains inferred from nuclear and chloroplast microsatellites confirms post-glacial range contraction and identifies introduced individuals

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