Genetic diversity and spread of Bovine leukaemia virus isolates in Argentine dairy cattle

Monti, Gustavo; Schrijver, R.S.; Beier, Dagmar

doi:10.1007/s00705-004-0437-1

Cited by 43 publications

(49 citation statements)

References 29 publications

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“…Thus, our genotypes 1, 2 and 4 are equivalent to clusters 2, 1 and 3 described by Camargos et al (2007) (Table 1). The sequences AF257515.1, K02120.1 and M35238.1, which clustered into genotypes 2, 1 and 4, were included in the first, third and second clusters described by Monti et al (2005) Table S1). Genotypes 1-6 identified here are indicated with lines surrounding the corresponding tree branches.…”

Section: Resultsmentioning

confidence: 99%

“…In fact, it has been shown that the sensitivity and specificity of different serological testing methods already demonstrate significant variation (Trono et al, 2001) and produce different results when compared with molecular methods (Fechner et al, 1996;Reichel et al, 1998). Furthermore, the existence of BLV strains that escape antibody detection, associated with the presence of particular genotypes, has been described (Fechner et al, 1997;Monti et al, 2005). Monti et al (2005) observed that 31 of 445 animals that tested positive by PCR were negative when screened by serological analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the env sequences of the BLV provirus from different locations worldwide have previously been classified into between two and four genetic groupings (Camargos et al, 2002(Camargos et al, , 2007Felmer et al, 2005;Hemmatzadeh, 2007;Licursi et al, 2003;Mamoun et al, 1990;Monti et al, 2005;Zhao & Buehring, 2007), with similar results obtained from analyses of the pol gene (Dube et al, 1997) and the four complete genomes available (Dube et al, 2000). Some of these groupings appear to correlate with the geographical origin of the strains (Camargos et al, 2002;Coulston et al, 1990;Felmer et al, 2005;Hemmatzadeh, 2007;Mamoun et al, 1990;Monti et al, 2005;Zhao & Buehring, 2007). Each individual study gave unique names to the identified groups and, as of yet, no comprehensive analysis has integrated all of the available sequences.…”

Section: Introductionmentioning

confidence: 99%

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Bovine leukemia virus can be classified into seven genotypes: evidence for the existence of two novel clades

Rodríguez¹,

Golemba²,

Campos³

et al. 2009

Journal of General Virology

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Previous studies have classified the env sequences of bovine leukemia virus (BLV) provirus from different locations worldwide into between two and four genetic groupings. These different studies gave unique names to the identified groups and no study has yet integrated all the available sequences. Thus, we hypothesized that many of the different groups previously identified actually correspond to a limited group of genotypes that are unevenly distributed worldwide. To examine this hypothesis, we sequenced the env gene from 28 BLV field strains and compared these sequences to 46 env sequences that represent all the genetic groupings already identified. By using phylogenetic analyses, we recovered six clades, or genotypes, that we have called genotypes 1, 2, 3, 4, 5 and 6. Genotypes 1-5 have counterparts among the sequence groupings identified previously. One env sequence did not cluster with any of the others and was highly divergent when compared with the six genotypes identified here. Thus, an extra genotype, which we named 7, may exist. Similarity comparisons were highly congruent with phylogenetic analyses. Furthermore, our analyses confirmed the existence of geographical clusters. INTRODUCTIONBovine leukemia virus (BLV) is a member of the family Retroviridae belonging to the genus Deltaretrovirus. This genus also includes Simian T-lymphotropic virus 1, 2, 3 and 5 (STLV-1, -2, -3 and -5) and Human T-lymphotropic virus 1, 2, 3 and 4 (HTLV-1, -2, -3 and -4). have not yet been associated with any pathology, likely due to their recent identification and to the low number of isolates. Therefore, BLV is considered a model of HTLV-1 and -2 . BLV is recognized as the aetiological agent of enzootic bovine leukosis, a disease that results in significant economic losses for the worldwide cattle industry. The most conspicuous clinical manifestation of bovine leukosis, which only develops in a small fraction of infected animals, is the clonal expansion and local accumulation of B cells that results in the development of lymphoid tumours (lymphosarcoma, LS) (Gillet et al., 2007). The majority of infections are not associated with any clinical signs (AL), and in approximately 30 % of infected cattle, the virus causes a persistent lymphocytosis (PL) (Burny et al., 1987;Mirsky et al., 1996).Analyses of the BLV envelope (env) gene of isolates collected in multiple geographical locations demonstrated significant sequence conservation (Camargos et al., 2002(Camargos et al., , 2007Coulston et al., 1990). Nevertheless, up to seven BLV genotypes can be identified by RFLP analysis (Asfaw et al., 2005;Coulston et al., 1990;Fechner et al., 1997;Kettmann et al., 1981;Licursi et al., 2002). Furthermore, the env sequences of the BLV provirus from different locations worldwide have previously been classified into between two and four genetic groupings (Camargos et al., 2002(Camargos et al., , 2007Felmer et al., 2005;Hemmatzadeh, 2007;Licursi et al., 2003;Mamoun et al., 1990;Monti et al., 2005;Zhao & Buehring, 2007), with similar results...

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bovine leukemia virus can be classified into seven genotypes: evidence for the existence of two novel clades

Rodríguez¹,

Golemba²,

Campos³

et al. 2009

Journal of General Virology

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“…En la primera reacción se usaron 25ng de ADN, 10mM de cada cebador "sentido" env 5032 5´-TCTGTGCCAAGTCTCCCAGATA-3´ y "anti sentido" env 5608r 5´-AACAACAACCTCTGGGAA-GGGT-3´, 0.2 mM de cada dNTP, 1X de tampón de PCR, 2.5 mM de MgCl 2 y 1U de Taq DNA Polymerase (Fermentas ® ). En la segunda reacción se utilizó como ADN molde 1µl del producto amplificado de la primera reacción, las mismas concentraciones de los demás reactivos y los cebadores internos "sentido" env 5099 5´-CCCACAAGGGCGGCGCCGGTTT-3› y "anti sentido" env 5521r 5´-GCGAGGCCGGGTCCAGAGCTGG-3´, haciendose ambas reacciones a un volumen final de 15µl (Monti et al, 2005). El perfil térmico de la primera reacción incluyó una desnaturalización inicial a 94°C durante 5 minutos, seguido por 40 ciclos de 94°C por 30 segundos, 60°C por 30 segundos y 72°C por 1 minuto, para terminar con una extensión final a 72°C durante 5 minutos.…”

Section: Presencia Del Vlb Por Métodos Moleculares (Env)unclassified

Dinámica de la Leucosis Bovina en el ganado criollo Hartón del Valle en infección natural

2016

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Carga proviral. Ganado criollo colombiano. Resistencia a enfermedades. resUMenLos bovinos criollos presentan características adaptativas de importancia. Se ha propuesto que el desarrollo y/o progresión de la leucosis bovina tiene bases genéticas. En el presente trabajo se estudió la dinámica del virus de la leucosis bovina en el ganado criollo Hartón del Valle en condiciones de infección natural. En 93 animales Hartón del Valle (HV), 30 Lucerna (LUC) y 30 Holstein (HOL) se evaluó la presencia del VLB por PCR-anidada, el desarrollo de linfocitosis por extendido en placa, los títulos de anticuerpos usando ELISA y la carga proviral mediante PCR en tiempo real, en tres muestreos durante un año. Se estimaron los porcentajes de animales infectados y se realizaron pruebas de independencia entre las variables evaluadas. Se encontró un efecto racial significativo sobre el desarrollo de la leucosis bovina, los animales de la raza Hartón presentaron menor tasa de infección del VLB (38,3%), desarrollaron menos linfocitosis persistente (15%), tuvieron mayores títulos de anticuerpos contra el virus (97,6) y mantuvieron la carga proviral más baja (663828 copias/ng de ADN) que las razas Lucerna y Holstein. Los datos aquí presentados pueden ser utilizados en programas de erradicación y control de la enfermedad. Se concluye que el ganado criollo Hartón del Valle puede ser considerado un progresor lento de la leucosis bovina. Dynamics of Bovine leukosis in creole cattle Hartón del Valle in natural infection INTRODUCCIÓNLa leucosis bovina enzoótica (LBE), es una enfermedad infecciosa producida por un retrovirus, el virus de la leucosis bovina (VLB), que afecta las células de la línea linfoide, los linfocitos "B" (Beyer et al., 2002; Baruta et al., 2011;). Es una enfermedad linfoproliferativa y se caracteriza por presentar linfocitosis persistente (LP) que se manifiesta con un incremento sostenido del número absoluto de linfocitos en la corriente sanguínea la cual aparece con una frecuencia de entre el 30 y 70% de los animales infectados; tumores (linfosarcoma o linfoma maligno) en los bovinos mayores a 3 años con una frecuencia de ocurrencia de entre 0.1 y 10% y una forma en la cual los animales tienen anticuerpos (anti-VLB) sin linfocitosis persistente, ni lesiones tumorales condición conocida como fase Aleucemica (Chamizo, 2005;Cadavid 2012). Entre otros síntomas se cuentan el aumento bilateral más o menos simétrico de los ganglios linfáticos explorables (Shell y Muller, 2004), la exoftalmia por la afección del tejido retro-ocular o de las estructuras internas del ojo, pueden considerarse como una manifestación específica de la enfermedad, así como la presencia de masas linfoadenopatías sub-

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“…In all sequences deletions and insertions were observed. Insertions and deletions were not observed in BLV pol and env sequences (Coulston et al 1990, Dube et al 1997, Dube et al 2000, Camargos et al 2002, Monti et al 2005. The sequences D00647 and K02120 probably had sequencing errors, as mentioned by others (Dube et al 2000).…”

Section: Resultsmentioning

confidence: 69%

Molecular Characterization of Partial Sequences of Brazilian BLV Gag Sequences

Camargos¹,

Reis²,

Leite³

2007

VR&R

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Genetic diversity and spread of Bovine leukaemia virus isolates in Argentine dairy cattle

Cited by 43 publications

References 29 publications

Bovine leukemia virus can be classified into seven genotypes: evidence for the existence of two novel clades

Bovine leukemia virus can be classified into seven genotypes: evidence for the existence of two novel clades

Dinámica de la Leucosis Bovina en el ganado criollo Hartón del Valle en infección natural

Molecular Characterization of Partial Sequences of Brazilian BLV Gag Sequences

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