Genetic diversity and structure of Schistosoma japonicum within two marshland villages of Anhui, China, prior to schistosome transmission control and elimination

Abstract

Schistosomiasis is caused by the genus Schistosoma and affected more than 250 million people worldwide. Schistosoma japonicum was once seriously endemic in China and nearly 60 years of efforts has seen great success in disease control. However, due to its zoonotic nature and complex life cycle, the schistosomiasis transmission control and final elimination would require, besides an intersectoral approach, deep understanding of population genetics of the parasite. We therefore performed a snail survey in two marshland villages of Anhui province of China and collected S. japonicum cercariae from infected snails. By using the recent developed microsatellite panel comprising seven loci, we genotyped the sampled parasites and analyzed the population genetic diversity and structure. The results showed much lower infection prevalence of S. japonicum in snails and low infected snail density in either marshland village. Through population genetic analyses, a considerable genetic diversity of parasites was revealed, whereas a small number of clusters were inferred and the sign of bottleneck effect was detected in each village. For the first time in S. japonicum in two villages, we provided estimates of effective population sizes with two different approaches. The results indicated that the parasite in two villages could eventually be eradicated with the ongoing integral control measures, but with potential risk of reinvasion of immigrant parasites through the Yangtze River. Such would be of great importance in assessment of the effects of ongoing control measures and prediction of the transmission capability for S. japonicum, thus guiding decisions on the choice of further control work.

The genetic variation of different developmental stages of Schistosoma japonicum: do the distribution in snails and pairing preference benefit the transmission?

“…Some anthropogenic changes could influence the genetic diversity and population structure of Schistosoma japonicum [ 5 – 8 ]. Geographical differentiation among S. japonicum populations in China has been observed using a variety of markers, including nuclear genes [ 9 , 10 ], mitochondrial genes [ 11 , 12 ], microsatellite loci [ 13 , 14 ], and genome-wide single nucleotide polymorphisms (SNPs) [ 15 , 16 ]. The population differentiation of S. japonicum was thought to result from geographical separation, habitat isolation [ 12 , 17 ], co-evolution with different species of snails [ 18 – 20 ], and different transmission patterns driven by definitive hosts [ 17 , 21 , 22 ].…”