Genetic diversity evaluation in wild Muntingia calabura L. based on Random Amplified Polymorphic DNA (RAPD) markers

Nasution, Fitriana; Theanhom, Araya Arjcharoen; Sukartini,; Bhuyar, Prakash; Chumpookam, J.

doi:10.1016/j.genrep.2021.101335

Cited by 10 publications

(3 citation statements)

References 25 publications

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“…Plant genetic diversity can be studied and detected with the help of molecular markers [21]. For genetic diversity studies in plants, a variety of molecular markers are used, including RAPD [22,23], ISSR [24], amplified fragment length polymorphism (AFLP) [25,26] and SCoT [27].…”

Section: Discussionmentioning

confidence: 99%

Genetic and Morphological Diversity Assessment of Five Kalanchoe Genotypes by SCoT, ISSR and RAPD-PCR Markers

Al-Khayri

Mahdy

Taha

et al. 2022

Plants

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Determining the appropriate parents for breeding programs is the most important decision that plant breeders must make to maximize the genetic variability and produce excellent recombinant genotypes. Several methods are used to identify genotypes with desirable phenotypic features for breeding experiments. In this study, five kalanchoe genotypes were morphologically characterized by assessing plant height, number of inflorescences, number of flowers, flower length, flower diameter and number of petals. The analysis showed the distinction of yellow kalanchoe in the plant height trait, while the orange kalanchoe was distinguished in the number of inflorescences, the number of flowers and flower length traits, whereas the violet kalanchoe possessed the largest flower diameter and the highest number of petals. The molecular profiling was performed by random amplified polymorphism DNA (RAPD), inter-simple sequence repeats (ISSR) and start codon targeted (SCoT)-polymerase chain reaction (PCR) tools. Genomic DNA was extracted from young leaves and the PCR reactions were performed using ten primers for each SCoT, ISSR and RAPD marker. Only four out of ten primers showed amplicon profiles in all PCR markers. A total of 70 bands were generated by SCoT, ISSR and RAPD-PCR with 35 polymorphic bands and 35 monomorphic bands. The total number of bands of RAPD, ISSR and SCoT was 15, 17 and 38, respectively. The polymorphism percentages achieved by RAPD, ISSR and SCoT were 60.25%, 15% and 57%, respectively. The cluster analysis based on morphological data revealed two clusters. Cluster I consisted of violet and orange kalanchoe, and cluster II comprised red, yellow and purple kalanchoe. Whereas the cluster analysis based on molecular data revealed three clusters. Cluster I included only yellow kalanchoe, cluster II comprised orange and violet kalanchoe while cluster III comprised red, and purple kalanchoe. The study concluded that orange, violet and yellow kalanchoe are distinguished parents for breeding economically valued traits in kalanchoe. Also, the study concluded that SCoT and RAPD markers reproduced reliable banding patterns to assess the genetic polymorphism among kalanchoe genotypes that consider the basis stone for genetic improvements in ornamental plants.

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Section: Discussionmentioning

confidence: 99%

Genetic and Morphological Diversity Assessment of Five Kalanchoe Genotypes by SCoT, ISSR and RAPD-PCR Markers

Al-Khayri

Mahdy

Taha

et al. 2022

Plants

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“…The geographical presence of M. calabura across the globe has been tabulated in Table 1. Taxonomy: Muntingia calabura is a pioneer species 18 . Muntingia calabura was previously classified as a member of the Elaeocarpaceae or Tiliaceae family.…”

Section: Mutengia Calabura Has Its Traditional Medicinal Values Since...mentioning

confidence: 99%

Traditional, Current and Prospective Therapeutic Uses of Muntingia calabura: A Comprehensive Literature Review

Saud,

Geetha,

Dubey

et al. 2023

Res. J. Med. Plants

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Muntingia calabura is a blooming evergreen plant. This plant has a unique traditional significance in different parts of the world. Based on traditional value pre-clinical research being conducted around the world, including identified phytochemicals responsible for various pharmacological activities. This review focuses on the taxonomical classification, worldwide geographical distribution, isolated constituents, traditional importance, therapeutic activity as well as the toxicity of the plant extract on animals. All the data included in this article was gathered from different online search engines. As a result of the survey signified that this plant has several traditional utilization-like headaches, tranquilizer, antispasmodic, including colds, gastric ulcers, antibacterial, insomnia and so on. Modern scientific studies have revealed that M. calabura has significant pharmacological properties, such as gastroprotective, anti-cancer, hepatoprotective, anti-bacterial and anti-diabetic, which are attributed to various phytochemicals found in various parts of the plant which are identified by using various techniques. This review focuses on ethnomedicinal applications, phytochemicals, toxicity and pharmacological properties, as well as continuing pre-clinical trials. Moreover, the review's findings will be valuable in building a full plant profile, which will support the investigator in subsequent research and fill in the gaps in clinical investigations.

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“…Based on the results, D. mirbelianum and D. lamellatum were in the same cluster but in different geographical areas of Java and Kalimantan. According to (Nasution et al 2021), geographical area has no impact on the genetic diversity of a species. Although different environmental conditions such as variations in soil, temperature, and rainfall cause distinct clusters of genotypes, populations from the same geographic area tended to cluster together.…”

Section: Cluster Analysismentioning

confidence: 99%

Genetic similarity among Dendrobium species from Indonesia using RAPD markers

HARTATI,

SAMANHUDI,

CAHYONO

2023

Biodiversitas

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Abstract. Hartati S, Samanhudi, Cahyono O. 2023. Genetic similarity among Dendrobium species from Indonesia using RAPD markers. Biodiversitas 24: 5015-5021. Dendrobium genus orchids are one of the most popular commodities in the world due to their diverse range of flower sizes, shapes, and colors. To enhance plant breeding programs and genetic resources, it is necessary to obtain information on genetic similarity between orchids of the Dendrobium genus through molecular analysis techniques. Therefore, this study aimed to assess the genetic similarity among five Dendrobium species using molecular markers, specifically RAPD. The plant material used was obtained from five species of the Dendrobium spp, namely (i) Dendrobium mirbelianum; (ii) Dendrobium lamellatum, (iii) Dendrobium secundum, (iv) Dendrobium bracteosum, and (v) Dendrobium purpureum. The analysis was carried out to determine the genetic diversity of the Dendrobium orchids using RAPD markers. A total of five RAPD primers were used for amplification, namely OPD 8, OPA 7, OPA 13, OPB 12, and OPB 18. The scoring data were analyzed using NTSYS-pc (Numerical Taxonomy and Multivariate Analysis System) version 2.02 which produced data in the form of cluster dendrograms. The dendrogram that was constructed by Unweighted Pair Group Method Using Arithmetic Average (UPGMA) classified the five Dendrobium species into two main clusters. The results showed that there were two clusters, namely Cluster A consisting of D. mirbelianum, D. lamellatum, and D. secundum while Cluster B consisted of D. bracteosum and D. purpureum. Furthermore, the polymorphism of the five RAPD primers was very high, ranging from 91.6 to 100%, measuring 250-1900 bp. The coefficient of genetic similarity analyzed using the five RAPD primers ranged from 0.24 to 0.77. The species D. mirbelianum and D. lamellatum had a high coefficient of genetic similarity. Which can be discovered through parental selection in breeding program.

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Genetic diversity evaluation in wild Muntingia calabura L. based on Random Amplified Polymorphic DNA (RAPD) markers

Cited by 10 publications

References 25 publications

Genetic and Morphological Diversity Assessment of Five Kalanchoe Genotypes by SCoT, ISSR and RAPD-PCR Markers

Genetic and Morphological Diversity Assessment of Five Kalanchoe Genotypes by SCoT, ISSR and RAPD-PCR Markers

Traditional, Current and Prospective Therapeutic Uses of Muntingia calabura: A Comprehensive Literature Review

Genetic similarity among Dendrobium species from Indonesia using RAPD markers

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