Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats

Frothingham, Richard; Meeker-O'Connell, Winifred A

doi:10.1099/00221287-144-5-1189

Cited by 516 publications

(478 citation statements)

References 25 publications

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“…The M. bovis strains were also analysed by mycobacterial interspersed repetitive units (MIRU) -variable-number tandem repeats (VNTR) aimed at loci ETR-A [10] and loci 4 [36].…”

Section: Molecular Characterisation Of M Bovis Isolatesmentioning

confidence: 99%

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

et al. 2006

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-The intradermal tuberculin (IDTB) test and the interferon-gamma (IFN-γ) assay are used worldwide for detection of bovine tuberculosis in cattle, but little is known about the effect of co-infecting agents on the performance of these diagnostic tests. This report describes a field trial conducted in a cattle herd with dual infection (bovine tuberculosis and paratuberculosis) during 3.5 years. It has been based on a strategic approach encompassing serial parallel testing (comparative IDTB test, the IFN-γ assay and serology of paratuberculosis) that was repeated 8 times over the period, and segregation of animals into two herds. The IDTB test detected 65.2% and the IFN-γ test detected 69.6% of the Mycobacterium bovis culture-positive cattle. However, the IDTB test performed better during the first part of the trial, while the IFN-γ test was the only method that detected infected animals during the following three samplings. The number of false positive reactors with the IDTB and/or the IFN-γ tests was remarkably high compared to other reports, and could be caused by cross-reactivity with M. avium subsp. paratuberculosis. Also, the M. bovis isolates from cattle and wildlife from the same property were characterised using molecular techniques to disclose an epidemiological link. The IDTB test may not be appropriate to eradicate bovine tuberculosis in herds with dual mycobacterial infections. This report highlights the need to use several diagnostic techniques for the accurate detection of M. bovis infected animals in these herds. tuberculosis / paratuberculosis / IDTB / IFN-γ / eradication

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“…The M. bovis strains were also analysed by mycobacterial interspersed repetitive units (MIRU) -variable-number tandem repeats (VNTR) aimed at loci ETR-A [10] and loci 4 [36].…”

Section: Molecular Characterisation Of M Bovis Isolatesmentioning

confidence: 99%

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

et al. 2006

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“…This allowed allocation of each isolate to one of the small number of readily identifiable M. bovis clones that occur in Britain (Smith et al, 2003); exploratory analysis revealed that an alternative typing method (using variable number tandem repeats; Frothingham and Meeker-O'Connell, 1998) provided no additional information.…”

Section: Diagnosis and Severity Of M Bovis Infection In Badgersmentioning

confidence: 99%

Bovine Tuberculosis in Cattle and Badgers in Localized Culling Areas

Woodroffe¹,

Donnelly

Cox³

et al. 2009

Journal of Wildlife Diseases

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ABSTRACT:Bovine tuberculosis (TB) is a zoonotic disease that can have serious consequences for cattle farming and, potentially, for public health. In Britain, failure to control bovine TB has been linked to persistent infection of European badger (Meles meles) populations. However, culling of badgers in the vicinity of recent TB outbreaks in cattle has failed to reduce the overall incidence of cattle TB. Using data from a large-scale study conducted in 1998-2005, we show that badgers collected on such localized culls had elevated prevalence of Mycobacterium bovis, the causative agent of bovine TB, suggesting that infections in cattle and badgers were indeed associated. Moreover, there was a high degree of similarity in the M. bovis strain types isolated from cattle and associated badgers. This similarity between strain types appeared to be unaffected by time lags between the detection of infection in cattle and culling of badgers, or by the presence of purchased cattle that might have acquired infection elsewhere. However, localized culling appeared to prompt an increase in the prevalence of M. bovis infection in badgers, probably by disrupting ranging and territorial behavior and hence increasing intraspecific transmission rates. This elevated prevalence among badgers could offset the benefits, for cattle, of reduced badger densities and may help to explain the failure of localized culling to reduce cattle TB incidence.

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“…Thirty-five polymorphic tandem repeat (TR) loci, ranging in size between 50 and 111 base pairs (bp), were reported to have potential value. 5,15,16,19 After preliminary testing, 3 loci (0580, 3006, 3191) were dropped from consideration because the primers for these loci amplified the same TR locus as other primers (0580S, 3007, 3192, respectively), and 5 loci (4156, 2074, 1612, 2059, 3155) were dropped because of multiple banding patterns or no amplification of product. These problems were not resolved by adjusting polymerase chain reaction (PCR) conditions or cycling parameters.…”

Section: Tandem Repeat Locimentioning

confidence: 99%

Genotyping North American Animal Mycobacterium Bovis Isolates Using Multilocus Variable Number Tandem Repeat Analysis

et al. 2008

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Abstract. Multilocus variable number tandem repeat analysis (MLVA) has recently emerged as a genotyping method that is both robust and highly discriminatory for the differentiation of Mycobacterium tuberculosis complex (MTBC) strains, including Mycobacterium bovis. However, MLVA assessment of M. bovis isolates recovered from animals in North America has been limited. Using an epidemiologically diverse set of 41 North American M. bovis animal isolates, MLVA, based on 27 published variable number tandem repeat (VNTR) loci, was evaluated. Nineteen loci displayed polymorphism, which resulted in differentiation of 21 unique MLVA genotypes. A subset of 6 loci differentiated the isolates into 14 genetically related groups that displayed remarkable concordance with the epidemiological data gathered via traditional trace-back methods. In most cases, MLVA exhibited greater resolution than spoligotyping, which differentiated the isolates into 11 groups. MLVA genotyping of M. bovis shows great potential as a molecular typing tool for characterizing the epidemiology of M. bovis animal infections in North America. However, the greatest resolution was achieved by using a combination of both MLVA and spoligotyping.

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Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats

Cited by 516 publications

References 25 publications

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

Bovine Tuberculosis in Cattle and Badgers in Localized Culling Areas

Genotyping North American Animal Mycobacterium Bovis Isolates Using Multilocus Variable Number Tandem Repeat Analysis

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