Genetic diversity in tomato (Solanum lycopersicum L.) genotypes revealed by simple sequence repeats (SSR) markers

Kaushal, Ashish; Singh, Anita; Jeena, A.S.

doi:10.31018/jans.v9i2.1305

Cited by 10 publications

(8 citation statements)

References 13 publications

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“…Primers CA and GT were not very informative. Kaushal et al (2017) found PIC values between 0,0 to 0,54 with an average of 0,27 in the evaluation of 25 tomato genotypes with 20 microsatellite markers. Likewise, Marin-Montes et al (2019), found an average PIC value of 0,63 when evaluating the genetic diversity of 26 accessions of native Mexican tomatoes with 18 SSR molecular markers.…”

Section: Resultsmentioning

confidence: 91%

Study of the genetic diversity of tomato (Solanum spp.) with ISSR markers

2020

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In this study 55 tomato genotypes from different geographical regions were evaluated with ISSR markers (Inter Simple Sequence Repeats). The seven ISSR primers originated 63 amplified bands, of which 90.48% were polymorphic. The cluster analysis based on the Nei-Li similarity coefficient using the average genetic clustering method (UPGMA) revealed the conformation of five clusters at a level of similarity of 72%. The ISSR technique did not discriminate tomato genotypes according to the species or region of provenance. The structure analysis and the dendrogram did not reveal a genetic structure in the population evaluated. The genotypes of the species of S. pimpinellifolium, S. l. cerasiforme, S. lycopersicum and S. peruvianum were found consistently grouped, showing a close genetic relationship among them. A high genetic variation among the individuals within each of the groups formed was suggested by the AMOVA. The ISSR markers were effective in assessing the genetic diversity and structure of populations of tomato genotypes. The high genetic variability found in this study indicates the valuable genetic potential present in tomato germplasm, especially of wild species, which could be used for future breeding programs of the species.

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Section: Resultsmentioning

confidence: 91%

Study of the genetic diversity of tomato (Solanum spp.) with ISSR markers

2020

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“…Pusa Ruby, IC-26372, Manulakshmi, Arka Samrat, Arka Alok, Sakthi showed 100% similarity. Kaushal et al (2017) reported a maximum of 96% similarity among tomato. High level of similarity (95%) was revealed among 39 tomato (Al-Abadi, 2007), similarity of 100% was found among tomato (Tam et al, 2005).…”

Section: Similarity Coefficient Analysismentioning

confidence: 98%

“…PCR reaction was performed in a 20μl reaction mixture which consisted of, 2.0 μl of genomic DNA with quantity 25ng/ μl, 2.0 μl of 10X Taq assay buffer A, dNTPs mix (10mm each) of about 1.5 μl, 0.3 μl of Taq DNA Polymerase (1U). 0.75 μl of Forward and reverse primer (10pM) respectively and 12.7 μl of autoclaved distilled water (Kaushal et al, 2017).…”

Section: Pcr Amplification Using Ssr Primersmentioning

confidence: 99%

High temperature induced changes in quality and yield parameters of tomato (Solanum lycopersicum L.) and similarity coefficients among genotypes using SSR markers

Vijayakumar

Shanija

Beena

et al. 2021

Heliyon

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High temperature induced by climatic fluctuations are an important threat for plant growth, development and quality of agricultural produces. Adaptableness to environmental changes generally derives from a large set of genetic traits affecting physio-morphological, biochemical and agronomic parameters. Therefore, the identification of genotypes with higher yield and good quality parameters at high temperatures is becoming increasingly necessary for future breeding programs. Here, we analyzed the performance of different tomato genotypes grown under elevated temperatures in terms of yield and nutritional quality of the fruit. High temperature stress was induced from flower initiation to maturity stage by keeping the pots in a temperature controlled green house facility for 45 days. The quality and yield parameters were taken at the harvesting stage. Starch and soluble sugar concentration in the leaves of tomato genotypes showed significant reduction in its amount under heat stress. Titrable acidity (TA), total soluble solids (TSS) and ascorbic acid content of tomato fruits were highest under high temperature conditions compared to ambient condition but lycopene content decreased with rise in temperature. The yield attributes viz., number of fruits/plant, fruit set %, average fruit weight (g), yield per plant (g/plant) were significantly lower for Arka Saurabh, Arka Rakshak and Pusa Rohini when compared to other genotypes under study. Molecular characterization of selected 22 tomato genotypes were assessed using 25 simple sequence repeat (SSR) markers. Phylogenetic tree was constructed by the unweighted neighbour-joining method (UPGMA) using NTSYSpc cluster analysis software. The Jaccard's similarity matrix was constructed using the SIMQUAL method using UPGMA algorithm in NTSYSpc. Jaccard's similarity matrix among these tomato genotypes ranged from a minimum of 0.22 to a maximum of 1 with an average genetic similarity of 0.67. Hence this study has importance in identifying genotypes that could maintain good quality and higher yield under high temperature condition.

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“…Briefly, microsatellites were used to analyze a wide array of traits like genetic diversity [16,17,18], organoleptic properties [19], delayed fruit ripening [20] to name a few.…”

Section: Applications Of Ssr and Issr Markers In Tomato Breedingmentioning

confidence: 99%

Applicability of ISAP, ISSR and SSR markers in tomato breeding programs

Панчев¹,

Aziz

Sarsu³

et al. 2019

Ovoŝi Rossii

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Domesticated crops are characterized by narrow genetic base reflecting one or more bottlenecks during millennia-long selection. As a result, current breeding programs are limited in available germplasm and are forced to deal with incremental improvements of yield, resistance, nutritional value, etc. Since the establishment of modern genetics and biotechnology, several new approaches have emerged to extend the genetic base and germplasm improvement. Among these methods, induced mutagenesis appeared as most useful conventional breeding tool. Although, its successful application currently requires good knowledge of modern molecular tools. In this paper we will make an attempt to overview SSR, ISSR and ISAP techniques as well as to offer examples of their application in tomato breeding programs.

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Genetic diversity in tomato (Solanum lycopersicum L.) genotypes revealed by simple sequence repeats (SSR) markers

Cited by 10 publications

References 13 publications

Study of the genetic diversity of tomato (Solanum spp.) with ISSR markers

Study of the genetic diversity of tomato (Solanum spp.) with ISSR markers

High temperature induced changes in quality and yield parameters of tomato (Solanum lycopersicum L.) and similarity coefficients among genotypes using SSR markers

Applicability of ISAP, ISSR and SSR markers in tomato breeding programs

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