Genetic Diversity inBrassica oleraceaL. (Cruciferae) and Wild Relatives (2n=18) using RAPD Markers

Genomic relationships between wild and cultivated Brassica oleracea L. with emphasis on the origination of cultivated crops

“…It supports the association of geographic distribution with the classification of wild species of B. oleracea reported previously (Lannér et al 1997;Lázaro and Aguinagalde 1998a).…”

“…Similar findings were documented in other studies. Evidence from the RAPD markers suggested that B. incana was excluded from the clade containing B. rupestris, B. insularis, B. macrocarpa and B. villosa (Lázaro and Aguinagalde 1998a). And the close relationship among B. rupestris, B. macrocarpa, B. villosa and B. insularis, and a more distant relationship with B. incana were also reported by Lannér (1998) and Geraci et al (2001).…”

Genetic diversity in the endangered Sicilian endemicBrassica rupestris: Proposals for a conservation strategy

“…In the case of B. rupestris, the level of genetic variability (H T ¼ 0.307, H S ¼ 0.212) was found to be high both at the species and at the population level, and comparable to those recorded for other (Lannér et al 1996;Lázaro & Aguinagalde 1998a, 1998b. The genetic variability levels of B. rupestris (GD ¼ 0.160-0.244) are similar to those of other plant species endemic to Mediterranean or Atlantic islands, such as Medicago citrina (Font Quer) Greuter (GD ¼ 0.035-0.143) (Juan et al 2004) and Armeria maderensis Lowe (GD ¼ 0.060-0.070, H T ¼ 0.140) (Piñ eiro et al (Minuto et al 2006).…”

“…The genetic variability levels of B. rupestris (GD ¼ 0.160-0.244) are similar to those of other plant species endemic to Mediterranean or Atlantic islands, such as Medicago citrina (Font Quer) Greuter (GD ¼ 0.035-0.143) (Juan et al 2004) and Armeria maderensis Lowe (GD ¼ 0.060-0.070, H T ¼ 0.140) (Piñ eiro et al (Minuto et al 2006). The high genetic variability detected in Brassica (H T ¼ 0.358) is probably due to the fact that species are often sympatric, forming a sort of melting pot (Lázaro & Aguinagalde 1998a, 1998b. This phenomenon definitely takes place in Sicily, a region that has been identified as a centre of genetic differentiation for the wild representatives of the Brassica oleracea group (Snogerup et al 1990;Lannér et al 1997).…”

EFFICIENCY OF RAPD AND ISSR MARKERS FOR GENOTYPE FINGERPRINTING AND GENETIC DIVERSITY STUDIES IN CANOLA (Brassica napus)

“…Numerous molecular markers have been used for variety identification in various plant species, which A allow cultivar identification in early stages of plant development, being neutral to environmental effects (Mohammadi, 2002;Meszaros et al, 2007;Moghaddam et al, 2009). A variety of molecular markers including Restriction Fragment Length Polymorphism (RFLP) (Thormann et al, 1994), Inter-Simple Sequence Repeats (ISSR) (Carolyn et al, 2000;Rudolph et al, 2002), amplified fragment length polymorphism (AFLP) (Sandip et al, 1999;Seyis et al, 2003;Jiang et al, 2007) and random amplified polymorphic DNA (RAPD) (Ashik Rabbani et al, 1998;Lazaro and Aguinagalde, 1998;Divaret et al, 1999), have been used to study the extent of genetic variation among the diverse group of important crop species in the genus Brassica (Afiah et al, 2007;Marjanovic-jeromela et al, 2009).…”