Genetic Diversity of Angelica gigas Nakai Collected in Korea using Genome-Wide SSR Markers

Jeong, Dong-Hyuk; Park, Yun Mi; Kim, Ki Yoon; Park, Hong Woo; Jeon, Kwon Seok; Kim, Mahn Jo; Gil, Jin Su; Lee, Yi; Um, Yurry

doi:10.7783/kjmcs.2019.27.6.376

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…In addition, A. dahurica harbors greater genetic diversity within population and a low genetic differentiation among populations as many outcrossing plants [37][38][39][40][41][42]. However, wild A. dahurica showed moderate genetic diversity (Ho = 0.516) among Apiaceae species (Ho ranged from 0.361 to 0.85, with an average of 0.634) using microsatellite markers [43][44][45][46][47]. Anthropic activities also have a huge impact on genetic diversity of wild plants as they are usually accompanied with dramatically increasing loss and damage of natural habitats [48][49].…”

Section: Discussionmentioning

confidence: 99%

Limited Genetic Diversity and High Differentiation in Angelica Dahurica Resulted by Domestication: Insights to Breeding and Conservation

Huang

Liu

Chen

et al. 2021

Preprint

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Background： Angelica dahurica, belonging to the Apiaceae family, whose dry root is a famous traditional Chinese medicine named as “Bai zhi”. There are two cultivars (A. dahurica cv. ‘Hangbaizhi’ and A. dahurica cv. ‘Qibaizhi’), which have been domesticated for thousands of years. Long term artificial selection has led to great changes in root phenotypes of the two cultivars, and also decreased their adaptability to environment. We proposed hypothesis that the cultivars may lose some genetic diversity and highly differentiate from wild A. dahurica during the domestication process. However, few studies have been carried out on how domestication affects the genetic variation of this species. Here, we accessed the levels of genetic variation and differentiation within and between wild A. dahurica and its cultivars using 12 SSR markers. Results: The results revealed that the genetic diversity of the cultivars was much lower than that of wild A. dahurica, and A. dahurica cv. ‘Qibaizhi’ had lower genetic diversity compared to A. dahurica cv. ‘Hangbaizhi’. AMOVA analysis showed significant genetic diﬀerentiation between the wild and cultivated A. dahurica, and between A. dahurica cv. ‘Hangbaizhi’ and A. dahurica cv. ‘Qibaizhi’. The results of Bayesian, UPGMA, NJ and PcoA clustering analysis indicated that all 15 populations were assigned to two genetic clusters corresponding to the wild and cultivated resources. Bayesian clustering analysis further divided the cultivated resources into two sub-clusters corresponding to the two cultivars. Conclusions：Our study suggests that domestication process is likely the major factor resulting in the loss of genetic diversity in cultivated A. dahurica and significant genetic differentiation from the wild resources due to founder effect and/or artificially directional selections. This large-scale analysis of population genetics could provide valuable information for genetic resources conservation and breeding programs of Angelica dahurica.

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Section: Discussionmentioning

confidence: 99%

Limited Genetic Diversity and High Differentiation in Angelica Dahurica Resulted by Domestication: Insights to Breeding and Conservation

Huang

Liu

Chen

et al. 2021

Preprint

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“…정적으로 탐색할 수 있으며 비교적 적은 비용으로 빠른 시간 안에 분석할 수 있다는 장점을 가진다(Jo et al 2013). 당귀 속 식물에서는 현재까지 RAPD 및 SSR 분자마커 등 을 사용한 종 식별에 대한 연구 사례들이 보고된 바 있으며(Jeong et al 2019;Lee et al 2001;Liao et al 2013;Mei et al 2015), 최근 본 연구팀에서는 핵내 리보솜 RNA를 암호 하는 유전자 단편 내 Internal Transcribed Spacer (ITS) 영역의 염기 서열을 비교하여 SNP를 확인하고 이를 이용한 Amplification Refractory Mutation System (ARMS)-PCR기술을 적용하여 실험에서 사용한 당귀 계통은 선행연구에서 사용된 국내 자 생하는 참당귀(Angelica gigas Nakai) 및 세발당귀(A. gigas Jiri), 일당귀(A. acutiloba), 중국당귀(A. sinensis), 및 구당귀(Levisticum officinale)를 대상으로 조사하였다(Lee et al 2021). 이들 계통 들은 국립원예특작과학원 인삼특작부, 경남농업기술원 약 용자원연구소, 경북농업기술원(봉화), 충북농업기술원(오창), 미국(horizon Herbs, Williams, OR)과 국내 한약재 유통 회사 등으로 부터 수집하였다.…”

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Development of specific single nucleotide polymorphism molecular markers for Angelica gigas Nakai

Lee

Han

et al. 2021

J Plant Biotechnol

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Angelica is a perennial plant used widely for medicinal purposes. Information on the genetic diversity of Angelica populations is important for their conservation and germplasm utilization. Although Angelica is an important medicinal plant genus registered in South Korea, no molecular markers are currently available to distinguish individual species from other similar species in different countries, in particular, China and Japan. In this study, we developed single nucleotide polymorphism (SNP) markers derived from internal transcribed spacer regions of the nuclear ribosomal DNA to identify a distinct domestic species, Angelica gigas Nakai, via a high-resolution melting (HRM) curve analyses. We also performed HRM curve analysis of intentionally mixed genomic DNA samples from five Angelica species. Finally, we investigated A. gigas Nakai and A. sinensis using varying ratios of mixed genomic DNA templates. The SNP markers developed in this study are useful for rapidly identifying A. gigas species from different countries.

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Limited genetic diversity and high differentiation in Angelica dahurica resulted from domestication: insights to breeding and conservation

et al. 2022

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Background Angelica dahurica belongs to the Apiaceae family, whose dry root is a famous traditional Chinese medicine named as “Bai zhi”. There are two cultivars (A. dahurica cv. ‘Hangbaizhi’ and A. dahurica cv. ‘Qibaizhi’), which have been domesticated for thousands of years. Long term artificial selection has led to great changes in root phenotypes of the two cultivars, and also decreased their adaptability to environment. We proposed hypothesis that the cultivars may have lost some of the genetic diversity found in the wild species and may be highly differentiated from the latter during the domestication process. However, few studies have been carried out on how domestication affected the genetic variation of this species. Here, we accessed the levels of genetic variation and differentiation within and between wild A. dahurica populations and two cultivars using 12 microsatellite markers. Results The results revealed that the genetic diversity of the cultivars was much lower than that of wild A. dahurica, and A. dahurica cv. ‘Qibaizhi’ had lower genetic diversity compared to A. dahurica cv. ‘Hangbaizhi’. AMOVA analysis showed significant genetic differentiation between the wild and cultivated A. dahurica populations, and between A. dahurica cv. ‘Hangbaizhi’ and A. dahurica cv. ‘Qibaizhi’. Results from Bayesian, UPGMA, NJ and PcoA clustering analysis indicated that all 15 populations were assigned to two genetic clusters corresponding to the wild and cultivated populations. Bayesian clustering analysis further divided the cultivated populations into two sub-clusters corresponding to the two cultivars. Conclusions Our study suggests that the domestication process is likely the major factor resulting in the loss of genetic diversity in cultivated A. dahurica populations and in significant genetic differentiation from the wild populations due to founder effect and/or artificially directional selections. This large-scale analysis of population genetics could provide valuable information for genetic resources conservation and breeding programs of Angelica dahurica.

show abstract

Genetic Diversity of Angelica gigas Nakai Collected in Korea using Genome-Wide SSR Markers

Cited by 6 publications

References 21 publications

Limited Genetic Diversity and High Differentiation in Angelica Dahurica Resulted by Domestication: Insights to Breeding and Conservation

Limited Genetic Diversity and High Differentiation in Angelica Dahurica Resulted by Domestication: Insights to Breeding and Conservation

Development of specific single nucleotide polymorphism molecular markers for Angelica gigas Nakai

Limited genetic diversity and high differentiation in Angelica dahurica resulted from domestication: insights to breeding and conservation

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