Genetic diversity of Bovine Viral Diarrhea Virus in cattle in France between 2018 and 2020

Rivas, José; Hasanaj, Alesia; Deblon, Caroline; Gisbert, Philippe; Garigliany, Mutien-Marie

doi:10.3389/fvets.2022.1028866

Cited by 13 publications

(14 citation statements)

References 58 publications

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“…Bovine pestiviruses can also be classified into subgenotypes based on phylogenetic analysis of full-length genome, 5′UTR sequences, or N pro and E2 analyses [ 3 ]. To date, several subgenotypes have already been described: BVDV-1 (1a–1x) [ 4 , 5 , 6 ], BVDV-2 (2a–2e) [ 7 , 8 , 9 ], and HoBiPeV (a–e) [ 10 , 11 ]. Moreover, BVDV can be classified into cytopathic (CP) and non-cytopathic (NCP) biotypes, according to their ability to induce cytopathic effect (CPE) during viral replication in cell culture.…”

Section: Introductionmentioning

confidence: 99%

HoBi-like Pestivirus Is Highly Prevalent in Cattle Herds in the Amazon Region (Northern Brazil)

Baumbach

Mósena

Alves

et al. 2023

Viruses

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Pestiviruses are globally distributed and cause substantial economic losses to the cattle industry. In Brazil, the country with the world’s largest cattle population, pestivirus infections are well described in some regions, such as in the south, where a high frequency of BVDV-2 is described and contrasts with the high prevalence of HoBi-like pestivirus (HoBiPeV) in the northeast. However, there is a lack of information about pestiviruses in the Amazon Region, in northern Brazil, with a cattle population estimated at 55.7 million head, which has a significant impact on the international livestock market. Therefore, this study investigated the seroprevalence and genetic variability of ruminant pestiviruses in 944 bovine serum samples from four states in northern Brazil: Pará (PA), Amapá (AP), Roraima (RR), and Amazonas (AM). Our results showed that 45.4% of the samples were seropositive (19.8% for BVDV-1, 14.1% for BVDV-2, and 20.9% for HoBiPeV). All samples were tested by RT–qPCR, and three were positive and classified as HoBiPeV in a phylogenetic analysis. These serological and molecular results contrast with those from other regions of the world, suggesting that the northern Brazilian states have a high prevalence of all bovine pestiviruses including HoBiPeV.

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Section: Introductionmentioning

confidence: 99%

HoBi-like Pestivirus Is Highly Prevalent in Cattle Herds in the Amazon Region (Northern Brazil)

Baumbach

Mósena

Alves

et al. 2023

Viruses

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“…The open‐reading frame, flanked by a 5′‐untranslated region (5′‐UTR) and a 3′‐UTR, can be translated into four structural proteins and eight nonstructural proteins [1]. According to the phylogenetic classification for BVDV strains, there are three main genetic populations, namely, BVDV‐1, ‐2, and ‐3 or Pestivirus A , B , and H [2–4]. Compared with the epidemic of BVDV‐2 and BVDV‐3, BVDV‐1 is the most widely distributed species around the world [5].…”

Section: Introductionmentioning

confidence: 99%

“…strains, there are three main genetic populations, namely, BVDV-1, -2, and -3 or Pestivirus A, B, and H [2][3][4]. Compared with the epidemic of BVDV-2 and BVDV-3, BVDV-1 is the most widely distributed species around the world [5].…”

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confidence: 99%

Analysis for codon usage bias in membrane anchor of nonstructural protein 5A from BVDV

Gao

Yang

et al. 2023

J Basic Microbiol

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The nonstructural protein 5A (NS5A) of the bovine viral diarrhea virus (BVDV) is a monotopic membrane protein. This protein can anchor to the cell membrane by an in‐plane amphipathic ⍺‐helix, which participates in the viral replication complex. In this study, the effects of synonymous codon usage pattern of NS5A and the overall transfer RNA (tRNA) abundance in cells on the formation of the in‐plane membrane anchor of NS5A were analyzed, based on NS5A coding sequences of different BVDV genotypes. BVDV NS5A coding sequences represent the most potential for BVDV genotyping. Moreover, the nucleotide usage of BVDV NS5A dominates the genotype‐specific pattern of synonymous codon usage. There is an obvious relationship between synonymous codon usage bias and the spatial conformation of the in‐plane membrane anchor. Furthermore, the overall tRNA abundance profiling displays that codon positions with a high level of tRNA abundance are more than ones with a low level of tRNA abundance in the in‐plane membrane anchor, implying that high translation speed probably acts on the spatial conformation of in‐plane membrane anchor of BVDV NS5A. These results give a new opinion on the effect of codon usage bias in the formation of the in‐plane membrane anchor of BVDV NS5A.

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“…Recent studies by Rivas et al . [ 5 ] and Sozzi et al . [ 6 ] have also identified new viruses that may belong to the genus Pestivirus .…”

Section: Introductionmentioning

confidence: 99%

Detection and characterization of bovine viral diarrhea virus in beef cattle imported from Australia to West Java, Indonesia

et al. 2023

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Background and Aim: To meet domestic demand for meat, Indonesia imports live cattle from Australia, which have non-bovine viral diarrhea (BVD)-free status. The consequence of importing live cattle from Australia is potentially introducing a novel BVD variant to Indonesia. Therefore, detecting BVD early and determining the agent’s characteristics and clinical symptoms are necessary. This study aimed to detect and characterize clinical symptoms of bovine viral diarrhea (BVD) and highlight the importance of farm management as a risk factor for the spread of BVD. This study aimed to provide information about the effectiveness of preventive measures against BVD in Australian-imported cattle at the Tanjung Priok Seaport Agricultural Quarantine. Bovine viral diarrhea is among the most common diarrheal diseases found in feedlots and is a severe health and economic problem in cattle. Materials and Methods: All cattle in a selected feedlot were examined for clinical symptoms on their first day of arrival. The sampling criteria included age, body weight, body temperature (BT), animal breath (AB), pulse (PL), conjunctivitis (CJ), hyperlacrimation (HL), hypersalivation (HS), DR, fever, limping leg (LL), emaciation, stomatitis (ST), weakness (WK), and coronitis (CR). In addition, 64 blood samples were taken from cattle that exhibited clinical symptoms of BVD. On the 3rd> day of arrival, a blood sample showing positive clinical symptoms was examined using antigen (Ag)-capture enzyme-linked immunosorbent assay (ELISA). The data from these clinical symptoms were analyzed alongside the laboratory results using multidimensional scale analysis, heatmap distribution, and principal component analysis (PCA). Furthermore, the positive serum samples obtained from the Ag-capture ELISA underwent a nested multiplex polymerase chain reaction and molecular detection and genetic characterization of BVDV based on the 5’ untranslated region of the viral genome, followed by sequence and phylogenetic tree analyses. Results: Using PCA, 12 clinical symptom characteristics of BVD were determined from 13 clinical symptoms synergized with five cattle positive for Ag-capture ELISA. The clinical symptoms included internal factors such as physiological conditions of CJ, HL, HS, DR, BT, LL, loss of appetite, ST, WK, CR, AB, and PL. The screening test showed that five samples tested positive for the BVD Ag, while 59 tested negative. Phylogenetic tree analysis using a 360-nucleotide portion of the NS5B gene showed that Sample B23F5R had a distinct path compared to the other two samples in the phylogenetic diagram. The profile of sample B23F5R was closely related to BVDV reference subgenotype 1-a group (NCBI, access no. LC068605), with a homology percentage of 92.36%. Furthermore, this sample was similar to the BVDV reference 1-a, Strain 12, identified in Japan. The other two samples, B13F5R and A13F5R, showed close resemblance to the BVDV reference subgenotype 1-a that had been previously identified in Indonesia (NCBI, access no. MK411755), with homology percentages of 97.81% and 97.75%, respectively. Conclusion: The BVDV-1a strain is the main subtype present in beef cattle imported from Australia to West Java, Indonesia. The characteristics of clinical symptoms associated with BVD infection comprised 12 symptoms synergized with the positive sample in the PCA. The present results can facilitate the development of preventive and control measures for BVD circulation in Indonesia. Keywords: bovine viral diarrhea virus, clinical symptoms, detection, imported cattle, West Java.

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Genetic diversity of Bovine Viral Diarrhea Virus in cattle in France between 2018 and 2020

Cited by 13 publications

References 58 publications

HoBi-like Pestivirus Is Highly Prevalent in Cattle Herds in the Amazon Region (Northern Brazil)

HoBi-like Pestivirus Is Highly Prevalent in Cattle Herds in the Amazon Region (Northern Brazil)

Analysis for codon usage bias in membrane anchor of nonstructural protein 5A from BVDV

Detection and characterization of bovine viral diarrhea virus in beef cattle imported from Australia to West Java, Indonesia

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