In order to assess genetic diversity of Brassica napus L., the seventy indigenous landraces along with two check cultivars (Shiralee and Pakola) were collected from different regions of Pakistan. The said germplasm was evaluated for 25 morphological and biochemical traits under field conditions during 2012 and 2013 at University of Haripur, Pakistan. Significant genotypic variability was observed among the tested landraces of B. napus for majority of traits during both years of studies. Among B. napus germplasm, the foremost genetic variability producing traits were, plant height and maturity, while adequate variations were recorded for flower initiation and completion, main raceme length and silique per main raceme and glucosinolate content. During 2012, cluster analysis divided the total 70 landraces of B. napus into nine main groups and contribution of five principal components (PCs) was 14.13, 13.11, 10.55, 9.85 and 8.25%, respectively. However, during 2013, the genotypes were categorized in seven main groups and the contribution of five PCs was 22.54, 16.69, 7.91, 6.54 and 6.11%, respectively. Based on two year studies, genotypes i.e., 24876, 24864, 24866 and 24901 were found promising with maximum genetic diversity and may be selected for future breeding program