Genetic Diversity of Clinical and Environmental Strains of
            <i>Salmonella enterica</i>
            Serotype Weltevreden Isolated in Malaysia

Thong, Kwai Lin; Goh, Y.L.; Radu, Son; Noorzaleha, S.; Yasin, Rohani Md; Koh, Y.T.; Lim, Victor; Rusul, Gulam; Puthucheary, S. D.

doi:10.1128/jcm.40.7.2498-2503.2002

Cited by 75 publications

(83 citation statements)

References 16 publications

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“…There are over 1.3 billion foodborne diarrhea cases occurred worldwide annually, resulting in 3 million deaths (Mastropeni and Maskell 2006). In Malaysia, the incidence rate of foodborne disease is about 8,640 cases reported by the Ministry of Health for the year 1999, where 811 (9.4%) cases were due to Salmonella infections (Thong et al 2002). Most Salmonella enterica serovars can cause illnesses in humans.…”

Section: Introductionmentioning

confidence: 99%

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Khoo

Cheah

Lee

et al. 2009

Antonie van Leeuwenhoek

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The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetables in Malaysia via an optimized, rapid and specific multiplex PCR assay. The assay encompasses a total of 19 Salmonella pathogenicity islands genes and a quorum sensing gene (sdiA) in three multiplex reaction sets. A total of 114 Salmonella enterica isolates belonging to 38 different serovars were tested. Each isolate in under this study was found to possess up to 70% of the virulence genes tested and exhibited variable pathogenicity gene patterns. Reproducibility of the multiplex PCR assay was found to be 100% and the detection limit of the optimized multiplex PCR was tested with lowest detectable concentration of DNA 0.8 pg microl(-1). This study demonstrated various Salmonella pathogenicity island virulence gene patterns even within the same serovar. This sets of multiplex PCR system provide a fast and reliable typing approach based on Salmonella pathogenicity islands, thus enabling an effective monitoring of emerging pathogenic Salmonella strains as an additional tool in Salmonella surveillance studies.

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Section: Introductionmentioning

confidence: 99%

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Khoo

Cheah

Lee

et al. 2009

Antonie van Leeuwenhoek

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“…Processing isolates using different restriction enzymes can yield distinct PFGE patterns and, subsequently, discordant interpretations of strain relationships (16). Genetic diversity in closely related outbreak strains (1,16) or clonally dominant endemic strains (17) may be insufficient to permit their correct interpretation, and some isolates with different phenotypes share the same PFGE pattern (18). PFGE results may correlate poorly with the true relatedness of isolates (16,19,20), especially among more distantly related strains (21), and at least one study reported that greater discriminatory power could be obtained through alternative molecular approaches (22).…”

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confidence: 99%

Application of Whole-Genome Sequencing for Bacterial Strain Typing in Molecular Epidemiology

et al. 2015

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Nosocomial infections pose a significant threat to patient health; however, the gold standard laboratory method for determining bacterial relatedness (pulsed-field gel electrophoresis [PFGE]) remains essentially unchanged 20 years after its introduction. Here, we explored bacterial whole-genome sequencing (WGS) as an alternative approach for molecular strain typing. We compared WGS to PFGE for investigating presumptive outbreaks involving three important pathogens: vancomycin-resistant Enterococcus faecium (n ‫؍‬ 19), methicillin-resistant Staphylococcus aureus (n ‫؍‬ 17), and Acinetobacter baumannii (n ‫؍‬ 15). WGS was highly reproducible (average < 0.39 differences between technical replicates), which enabled a functional, quantitative definition for determining clonality. Strain relatedness data determined by PFGE and WGS roughly correlated, but the resolution of WGS was superior (P ‫؍‬ 5.6 ؋ 10 ؊8 to 0.016). Several discordant results were noted between the methods. A total of 28.9% of isolates which were indistinguishable by PFGE were nonclonal by WGS. For A. baumannii, a species known to undergo rapid horizontal gene transfer, 16.2% of isolate pairs considered nonidentical by PFGE were clonal by WGS. Sequencing whole bacterial genomes with single-nucleotide resolution demonstrates that PFGE is prone to false-positive and false-negative results and suggests the need for a new gold standard approach for molecular epidemiological strain typing.

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“…S. weltevreden was isolated from beef and was similar reported from Thailand (Bangtrakulnonth et al 2004) and has been the most prevalent bovine Salmonella serovars in Vietnam (Vo et al 2006). In Malaysia, food poisoning cases reported by the Ministry of Health for the year 1999, of 8,640 cases, 811 (9.4%) were due to Salmonella (Thong et al 2002). In addition, report of food poisoning from Division of Food Safety and Quality, Department of Health Kelantan showed the result in 2009, in 11 of 56 episodes (19.6%), Salmonella was isolated from food samples collected during the outbreak investigation.…”

Section: Resultsmentioning

confidence: 67%

Prevalence of Pathogenic Foodborne Bacteria from Beef in Retail Stalls in Kelantan

Fauzi¹,

Arshad²,

Ruhil³

et al. 2016

Proceedings of International Seminar on Livestock Production and Veterinary Technology

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Salmonella, E. coli O157:H7, Methicillin-resistant Staphylococcus aureus (MRSA) and Listeria monocytogenes are common pathogenic foodborne bacteria causing foodborne illness in humans. The objective of this study was to determine the prevalence of those bacteria in beef from retail stalls in Kelantan. Beef samples were collected from retail stalls (roadside stalls and wet markets) and processed for the isolation and identification of Salmonella, E. coli O157:H7, MRSA and L. monocytogenes. Out of 25 beef samples from retail stalls, 11 (44%) were positive for Salmonella, 11 (44%) positive for E. coli and 8 (32%) positive for S. aureus. None of the beef samples contained E. coli O157:H7, MRSA and L. monocytogenes. Of 11 Salmonella isolated, the most common were S. mbandaka (46%), S. albany (36%) followed by S. weltevreden (18%). In this study, Salmonella, E. coli and S. aureus were found to be the most common foodborne bacteria from beef in retail stalls in Kelantan. Therefore, consumers have to practice a good safety foodhandling during purchasing, transporting and preparing the beef in the kitchen to prevent the risk of food poisoning.

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Genetic Diversity of Clinical and Environmental Strains of Salmonella enterica Serotype Weltevreden Isolated in Malaysia

Cited by 75 publications

References 16 publications

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Application of Whole-Genome Sequencing for Bacterial Strain Typing in Molecular Epidemiology

Prevalence of Pathogenic Foodborne Bacteria from Beef in Retail Stalls in Kelantan

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