Genetic Diversity of Cultivated Barley Landraces in Iran Measured Using Microsatellites

Khodayari, Hamed; Saeidi, H.; Roofigar, Azadeh Akhavan; Rahiminejad, Mohammad Reza; Pourkheirandish, Mohammad; Komatsuda, Takao

doi:10.7763/ijbbb.2012.v2.118

Cited by 16 publications

(23 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we employed 33 mapped SSR markers to evaluate the structure of the population and genetic diversity in 100 selected Pakistani genotypes with approved cultivars of barley for core relationship of these genotypes with each other and approved cultivars. On average, the SSR mapped markers amplified 4 alleles per SSR, with a range from 2 to 6 alleles per SSR, which was comparable to the value (4 in average) of the 33 SSR which is similar to the study of [16] on Iranian barley landraces. Our study up to some similarity are similar to the previously published reports of [21] which the conducted their study on Indian accessions of barley and lower than the ones published by others [11][12][13][14]17] Bmac0181, Bmag0120, Bmag0125, Bmag0603, Bmac0156, Bmag0900 and Bmag0872, were highly polymorphic, each producing 6 to 5 alleles.…”

Section: Discussionsupporting

confidence: 80%

Microsatellite Based Diversity Analysis and the Development of Core-Set Germplasm in Pakistani Barley Lines

Ali

Yang²,

Hussain

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Illustrating the population structure and genetic diversity in selected germplasm resources (after three year multi locations trials) plays a key role which directly utilize the selection of lines in a population for accumulative trait breeding in crops.Results In order to further understand, the structure of population and genetic variability, we explored a core set of 100 lines (selected from 600 genotypes) in multi-location trials for three years (2016-2019) in Dir Lower, Swat, Khyber Pukhtonkhwa Pakistan and China with 33 SSR markers. The integrated population structure analysis in a core of hundred germplasm with Pakistani origin with three approved commercial barley cultivars have strong stratification that allowed their division into four major subpopulations (i.e. PI, PII, PIII and PIV) and an admixture subpopulation, with 52, 9, 15 and 27 germplasm respectively. A total of 133 alleles were identified with mean value of 0.80 Polymorphic information content. The number of alleles detected by the system varied from two alleles amplified to as six with an average of 4.03 per SSR marker pair. The gene diversity ranged from 0.56 to 0.98 with an average of 0.82 in selected germplasm resources. Based on the SSR data, the 100 selected germplasm with three cultivars were classified into four main phylogenetic Linages (LI, LII, LIII and LIV) which corresponded to the phylogenic grouping in genotypes.Conclusion We assembled a core set of 20 barley genotypes (~1/5 of original population size) to sustain sufficient mapping of SSR marker with Phenotype, in which we proposed four SSR markers, Bmac0040, Bmac0134, Bmag0125 and Bmag0211 for malt gene and marker (Bmac0399) for tolerance to salinity gene, which will be applicable for marker assisted breeding in barley gene resources.

show abstract

Section: Discussionsupporting

confidence: 80%

Microsatellite Based Diversity Analysis and the Development of Core-Set Germplasm in Pakistani Barley Lines

Ali

Yang²,

Hussain

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…1). Comparable patterns for genetic structure were observed for Landraces in Iran (Khodayari et al, 2012) and landrace populations of barley in Sardinia (Rodriguez et al, 2012), with weak relationships between genetic structure and geographic origin. This analysis confirms genetic differentiation results and shows that the lowest genetic proximity was observed between Kasserine (central west) and Sfax (south) genepools.…”

Section: Population Genetic Structure and Conservation Strategymentioning

confidence: 81%

“…Our results agree with those of Jensen et al (2013), who reported low genetic structure according to geographic origin for Moroccan barley landraces. Comparable patterns for genetic structure were observed for Landraces in Iran (Khodayari et al, 2012) and landrace populations of barley in Sardinia (Rodriguez et al, 2012), with weak relationships between genetic structure and geographic origin. The Bayesian cluster analysis corroborates 3D AFC and gene flow results.…”

Section: Population Genetic Structure and Conservation Strategymentioning

confidence: 81%

Low Genetic Differentiation and Evidence of Gene Flow among Barley Landrace Populations in Tunisia

et al. 2017

View full text Add to dashboard Cite

Tunisian barley (Hordeum vulgare L.) landraces, representing the oldest cultivated accessions, are growing in scattered populations across drought‐ and salt‐stressed environments and constitute a precious reservoir of potentially useful traits for breeding programs. The objective of this study was to elucidate genetic diversity and population structure of barley landraces across the landscape of Tunisia. Populations from 11 geographic zones were genotyped using 21 nuclear microsatellites. A high level of genetic polymorphism with 170 detected alleles was recorded among the studied genotypes. The average allelic richness was 8.095 alleles per locus. The index of genetic diversity (He) showed an average of 0.741. Genetic diversity was very high within populations, whereas differences among populations were difficult to detect. Only 0.15% of the DNA variation was apportioned among landraces (P < 0.001), whereas 99.85% of the DNA variation was maintained within these landraces. A high gene flow (Nm) was revealed among the investigated populations, which has been facilitated by exchange of barley seeds between Tunisian cereal farmers of different regions. Genetic diversity within Tunisian barley landrace germplasms may help to maintain adaptation to a broad range of environmental conditions and provide genetically diverse resources for barley breeders. Both ex situ (seed banks) and in situ (on‐farm) conservation strategies may be required to maintain barley landrace genetic resources.

show abstract

“…These SSR primers totally produced 67 alleles ranging from two to six alleles per locus with a mean value of 3.94 alleles per locus. Also, Khodayari et al (2012) evaluated the genetic diversity of 32 individuals of tworowed and six-rowed Iranian landraces barley using 17 microsatellite markers. A high level of polymorphism information content (PIC; average = 0.651) and an average of 8.1 allele per locus were observed.…”

Section: Data Inmentioning

confidence: 99%

Genetic Diversity and Relationships Among Some Barley Genotypes for Net Blotch Disease Resistance Using Rapd, Scot and SSR Markers

Dora¹,

Mansour²,

Aboulila³

et al. 2018

Egyptian Journal of Genetics and Cytology

View full text Add to dashboard Cite

Genetic Diversity of Cultivated Barley Landraces in Iran Measured Using Microsatellites

Cited by 16 publications

References 16 publications

Microsatellite Based Diversity Analysis and the Development of Core-Set Germplasm in Pakistani Barley Lines

Microsatellite Based Diversity Analysis and the Development of Core-Set Germplasm in Pakistani Barley Lines

Low Genetic Differentiation and Evidence of Gene Flow among Barley Landrace Populations in Tunisia

Genetic Diversity and Relationships Among Some Barley Genotypes for Net Blotch Disease Resistance Using Rapd, Scot and SSR Markers

Contact Info

Product

Resources

About