Genetic diversity of flax genotypes (Linum usitatissimum L.) by using agro-morphological properties and molecular markers

Abstract

Flax is one of the signi cant crops owing to oilseed and bre attributes, exhibiting a wide distribution in many parts of the world. The present study was designed to reveal the genetic diversity of 29 genotypes of ax through morphological traits and 12 iPBS and 33 ISSR markers. According to the UPGMA-based dendogram, genotypes of the ax were classi ed into the two major groups (A and B) and group B was composed of two sub-groups (B1 and B2) according to the six phenotypic attributes considered for analysis. Concerning molecular relationships of the genotypes, individual and combined UPGMA-based dendograms were constructed with respect to the ISSR and iPBS markers. According to the Jaccard similarity coe cients for ISSR data, ax genotypes were divided into two main groups (A and B) and the "Van-1" genotype was in group A alone. Also, group B was divided into two separate subgroups. "Afyon-1" and "İzmir-Kemeraltı" genotypes were located in group B1, while all the remaining genotypes were located in group B2. In addition, the average genetic similarity was 0.755 regarding the ndings of iPBSretrotsposon markers, ax genotypes were classi ed into two main groups (A and B), and these main groups formed two subgroups among themselves. While "Konya-1" is located alone in A1 subgroup, "Iğdır", "İzmir-Kemeraltı", "Mardin", "Bitlis" and "Afyon-1" genotypes are located in A2 subgroup. While "Van-1" genotype was located in the B2 subgroup alone, other genotypes were found in the B2 subgroup. The average genetic similarity was determined as 0.578 according to Jaccard binary similarity coe cient. According to the combined data of two markers, two separate groups (A and B) were revealed, similar to the dendrogram constructed with ISSR data. "Van-1" genotype was located in group A alone.Group B was divided into two subgroups (B1 and B2). The average genetic similarity was 0.722 according to the Jaccard similarity coe cient of matrix.

Revealing Genetic Diversity and Population Structure in Türkiye’s Wheat Germplasm Using iPBS-Retrotransposon Markers

“…The genes that provide resistance to diseases, pests, and environmental pressures may be found in many genetic sources, even when these genes are not present in the crop types that are now in use [13]. These valuable genes may be present in wild species and traditional landraces [14,15]. The remaining portions of these gene pools are commonly referred to as genetic resources, as emphasized in the current literature [11].…”

“…The concept of utilizing iPBS-retrotransposons as a comprehensive marker applicable to both animal and plant species was introduced by Kalendar et al [31]. iPBS-retrotransposons have since been employed in molecular characterization, phylogenetic analysis, and evolutionary research across a variety of crop plants [15].…”