Genetic diversity of hybrid durian resulted from cross breeding between <i>Durio kutejensis</i> and <i>Durio zibethinus</i> based on random amplified polymorphic DNAs (RAPDs)

Abstract

One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has golden yellow fruit flesh without smell. Crossbreeding of those two cultivars, in order to generate superior Durian cultivars has been done. Genetic diversity of durian generates from cross breeding between D. kutejensis and D. zibethinus was identified in molecular level using RAPD technique. Among 20 primers used in this study, 5 primers: OPA-02, OPA-03, OPA-08, OPA-10 and OPA-13 were capable of differentiating both the parents and the hybrids. RAPD analysis resulted in genetic diversity of hybrid Durian with family relationship of 0.59% -0.1%. Hybrids UB1, UB5, UB13, UB19, UB21, UB7 and UB35 have similarity value of 0.81% with their parent DRCK, whereas hybrids UB8, UB10, UB18 and UB17 have similarity value of 0.70% with their parent DRCM1. Hybrids UB2, UB16 and UB22 belong to one group with similarity value of 0.67%. Three hybrids lines UB2, UB16 and UB22 show the highest distance to both parent. The rest of the hybrids lines grouped into similar cluster to the parents D. kutejensis, whereas the other parent (D. zibethinus) belong to different cluster separated from all other hybrid lines and parents.

Genetic variability of Indonesian local chili pepper: The facts

“…RAPD bands have been reported as capable of identifying differences between varieties. 8,[14][15][16][17][18][19][20][21] …”

“…This method has a weakness in that its repetition power is low 14 . This method is widely reported in analyzing genetic variations in various plants such as pamelo oranges 14 , kenaf (Hibiscus cannabinus) 15 , soybean16, durians 17,18 , and various species of chili or genus Capsicum like C. annum L. 8,[19][20][21] . Therefore, this research aimed to investigate genetic variation among the six commercial chili peppers found at the Malang market, based on morphological variations and RAPD markers.…”

Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker

“…The fruit of Lai is odorless or has a smooth aroma, covered by blunt spines, and has attractive flesh color from yellow to reddish orange (Antarlina 2009;Santoso 2010) that represents the high content of carotene and vitamin A (Antarlina 2009). Lai has longer fruit storage period (Antarlina 2009;Santoso 2010) compared to its close relative durian (Durio zibethinus) (Hariyati et al 2013) within which it becomes one of the prospective fruits for export purposes.…”

The Survival Rate of Grafted-Seedling of Durian (Durio zibhetinus Murr.) in the Indigenous Agroforestry Orchards of Osingnese in Banyuwangi, East Java, Indonesia

“…is native to southeastern Asia [1]. In Southeast Asia region, Indonesia is one of the centers for genetic diversity of durian [2].The genetic variety of durian in Indonesia provides important germplasm for breeding durian plants in Indonesia [3]. In Indonesia, durians mostly found in home garden of local people, especially home garden and orchards which area managed through agro forestry systems.…”