Genetic diversity of
            <i>Procambarus clarkii</i>
            populations based on mitochondrial DNA and microsatellite markers in different areas of Guangxi, China

Zhong, Yang; ZhangSheng, Tang; Huang, Liming; Wei, Dapeng; Lu, Zhuan-Ling

doi:10.1080/24701394.2020.1721484

Cited by 11 publications

(8 citation statements)

References 43 publications

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“…The species is highly adaptable and can disperse widely in the habitat and tolerate diverse environmental conditions [4,10]. Furthermore, P. clarkii has retained high levels of genetic diversity in both wild populations [5,6,9,11,12] and commercial populations [13]; this contributes to avoiding the harmful effects of inbreeding, for adapting to different environments [14], and in the selection of good breeding germplasm for crayfish artificial culture [5]. At present, P. clarkii farming has become an important industry in China, with production reaching 1,638,700 tons and a total output value of 369 billion China Yuan (CNY) in 2018 [15].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii

et al. 2021

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Background The red swamp crayfish Procambarus clarkii is a freshwater species that possesses high adaptability, environmental tolerance, and fecundity. P. clarkii is artificially farmed on a large scale in China. However, the molecular mechanisms of ovarian development in P. clarkii remain largely unknown. In this study, we identified four stages of P. clarkii ovary development, the previtellogenic stage (stage I), early vitellogenic stage (stage II), middle vitellogenic stage (stage III), and mature stage (stage IV) and compared the transcriptomics among these four stages through next-generation sequencing (NGS). Results The total numbers of clean reads of the four stages ranged from 42,013,648 to 62,220,956. A total of 216,444 unigenes were obtained, and the GC content of most unigenes was slightly less than the AT content. Principal Component Analysis (PCA) and Anosim analysis demonstrated that the grouping of these four stages was feasible, and each stage could be distinguished from the others. In the expression pattern analysis, 2301 genes were continuously increase from stage I to stage IV, and 2660 genes were sharply decrease at stage IV compared to stages I-III. By comparing each of the stages at the same time, four clusters of differentially expressed genes (DEGs) were found to be uniquely highly expressed in stage I (136 genes), stage II (43 genes), stage III-IV (49 genes), and stage IV (22 genes), thus exhibiting developmental stage specificity. Moreover, in comparisons between adjacent stages, the number of DEGs between stage III and IV was the highest. GO enrichment analysis demonstrated that nutrient reservoir activity was highest at stage II and that this played a foreshadowing role in ovarian development, and the GO terms of cell, intracellular and organelle participated in the ovary maturation during later stages. In addition, KEGG pathway analysis revealed that the early development of the ovary was mainly associated with the PI3K-Akt signaling pathway and focal adhesion; the middle developmental period was related to apoptosis, lysine biosynthesis, and the NF-kappa B signaling pathway; the late developmental period was involved with the cell cycle and the p53 signaling pathway. Conclusion These transcriptomic data provide insights into the molecular mechanisms of ovarian development in P. clarkii. The results will be helpful for improving the reproduction and development of this aquatic species.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii

et al. 2021

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“…Qianjiang City of the Hubei province has been recognized as the hometown of red swamp crayfish in China by the Ministry of Agriculture of the People's Republic of China [57]. The artificial breeding and culture model of crayfish is considered to be a popular model of crayfish production [5]. A population breeding strategy was applied, and several generations have been performed to obtain good characteristics, such as growth, disease resistance and suitability [58,59].…”

Section: Discussionmentioning

confidence: 99%

“…The red swamp crayfish is one of the most aggressive species in the world, with their dredging behavior causing damage to dikes, dams and rice paddies, but due to its high market value, including edible meat, high protein content, low fat content and high nutritional power, it is widely loved and consumed in China and is one of the most economically important aquatic species for aquaculture rather than a destructive invasive species [4]. Recently it has recently become a popular freshwater aquaculture species in China, especially in the middle and lower reaches of the Yangtze River [5]. In the past two decades, red swamp crayfish farming in rice fields has become the most commonly farming mode because it can improve ecological conditions and raise the production values of rice fields.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (Procambarus clarkii) from Lakes and Rice Fields by SSR Markers

Guo

Liu

Zhou

et al. 2022

Fishes

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The red swamp crayfish (Procambarus clarkii) is an important aquatic animal and has developed as a popular aquaculture species in China. In this study, a total of 72,839 SSR motifs were identified from transcriptional data, and 20 microsatellite markers of them were finally developed to assess the genetic diversities of seven wild populations from natural lakes and nine cultured populations from rice fields. Genetic diversity was slightly higher in the cultured populations than in the wild populations. The degree of genetic differentiation between cultured populations is slight, while a moderate to a large degree of genetic differentiation between wild populations and most of the variations occurred within individuals (79%). The analysis of cluster, principal coordinate analysis and STRUCTURE were similar, and they showed that isolation-by-distance pattern was not significant. The microsatellite markers developed in this study can not only be used for genetic monitoring of population but also provide important information for the management of breeding and cultured population in red swamp crayfish.

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“…There are only few population genetic studies dealing with the red swap crayfish invasion in the European continent based on different molecular markers [27][28][29][30][31]. Mitochondrial DNA (mtDNA) is considered one of the most useful markers to evaluate population dynamics [32][33][34]. The uniparental inheritance, the high mutation rate and the large number of copies inside the cells, make the mtDNA a very easy and cheap tool to identify the population variations.…”

Section: Introductionmentioning

confidence: 99%

Population Ecology and Genetic Diversity of the Invasive Alien Species Procambarus clarkii in Lake Trasimeno (Italy)

Dörr

Scoparo

Cardinali

et al. 2021

Biology

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The deliberate or accidental introduction of invasive alien species (IAS) causes negative ecological and economic impacts altering ecosystem processes, imperiling native species and causing damage to human endeavors. A monthly monitoring program was performed in Lake Trasimeno (Central Italy) from July 2018 to July 2019 in order to provide an upgrade of the population ecology of Procambarus clarkii and to assess the genetic diversity by analyzing the relationships among mitochondrial DNA diversity. Our results confirmed that P. clarkii is well acclimatized in the lake, revealing a stable population structure favored by the resources and conditions typical of this ecosystem, which seem to be optimal for the maintenance of the species. Four distinct mitochondrial haplotypes were detected, but one of them was clearly overrepresented (76%), suggesting that a single predominant introduction event may have occurred in this area, likely followed by secondary events. The identification of the typical genetic variants provides a better understanding of the evolutionary scenarios of P. clarkii in this biotope and it can be helpful in management plans concerning the expanding populations of this invasive alien species.

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Genetic diversity of Procambarus clarkii populations based on mitochondrial DNA and microsatellite markers in different areas of Guangxi, China

Cited by 11 publications

References 43 publications

Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii

Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii

Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (Procambarus clarkii) from Lakes and Rice Fields by SSR Markers

Population Ecology and Genetic Diversity of the Invasive Alien Species Procambarus clarkii in Lake Trasimeno (Italy)

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