Genetic Diversity of Iranian Accessions, Improved Lines of Chickpea (Cicer arietinum L.) and Their Wild Relatives by Using Simple Sequence Repeats

Abstract

Biodiversity information in available germplasm is very useful for the success of any breeding program. To establish genetic diversity among 44 genotypes of chickpea comprising cultigen, landraces, internationally developed improved lines and wild relatives, genetic distances were evaluated using 19 simple sequence repeat markers with 100 marker loci. Estimation of the number of alleles per locus (n a ), the effective allele number (n e ), and Wright fixation index F were 6.25, 3.67, and 0.44, respectively.Polymorphism information content values ranged from 0.84 (locus NCPGR6 and TA135) to 0.44 (locus NCPGR7) with an average of 0.68. Dice's coefficient similarity matrix for studied chickpea genotypes varied from 0.07 to 1.0 indicating a broader genetic base among genotypes studied. The highest similarity, 1.0, was observed between genotypes Sel 96TH11484 and Sel 96TH11485; while, the lowest, 0.07, was observed between genotypes Sel 95TH1716 and Azad. Based on the UPGMA clustering method, all genotypes were clustered in eight groups, which indicated the probable origin and region similarity of landraces and local Iran landraces over the other cultivars and wild species. It also represents a wide diversity among available germplasm. Analysis of molecular variance revealed that 41% of the total variance was due to differences among groups while 59% was due to differences within groups. The results of principal coordinate analysis approximately corresponded to those obtained through cluster analysis. Genetic variation detected in this study can be useful for selective breeding for specific traits and in enhancing the genetic base of breeding programs.

Simple sequence repeat markers associated with agro-morphological traits in chickpea (Cicer arietinum L.)

“…Detailed information on the levels of genetic diversity of genotypes is available elsewhere (Saeed et al, 2011). Analysis of population structure distinguished 6 subspecific populations (Fig.…”

“…The present study used 16 pairs of SSR primers and subdivided the association population into six subpopulations using the modelbased cluster method. Data from our genetic diversity (Saeed et al, 2011) and population structure analysis revealed that this association population showed a diverse genetic variation and, therefore, could be used for the association analysis. Among factors influencing marker-traits association, the admixture of populations is very important (Zhang et al, 2012).…”

Study of genetic diversity in local rose varieties (Rosa spp.) using molecular markers

“…but was not consistent with reports of Byrne, and Matsumoto and collab. who reported a low level of genetic diversity within rose germplasm [SAEED et al, 2011, GHAFFARI et al, 2014, BYRNE, 1999, MATSUMOTO et al, 1998] .…”

Assessment of genetic diversity among 125 cultivars of chickpea(Cicer arietinum L.) of Indian origin using ISSR markers

“…It is cultivated in about 33 countries and regions including Central-West Asia, South Europe, Ethiopia, North Africa, and Australia (FAO, 2013;ICRISAT, 2013). It has high nutritive value and serves as an important cheap source of protein in developing countries in addition to improving land fertility (Saeed et al, 2011). The chickpea seed is a good source of carbohydrates and proteins, which collectively constitute 80% of the total dry seed weight (Talebi et al, 2008;Aggarwal et al, 2013).…”