Genetic Diversity of Native Bradyrhizobia Isolated from Soybeans (
            <i>Glycine max</i>
            L.) in Different Agricultural-Ecological-Climatic Regions of India

Appunu, C.; N’Zoué, Angèle; Laguerre, Gisèle

doi:10.1128/aem.01320-08

Cited by 87 publications

(77 citation statements)

References 28 publications

(35 reference statements)

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“…In another study by Satya Prakash and Annapurna (2006) also reported occurrence of soybean rhizobia that are distinct from soybean rhizobia of USA and Brazilian origin based on BOX-PCR analysis. This was further supported by occurrence of distinct rhizobia in tropical soils of India (Appunu et al 2008). Thus, remarkable differences among the rhizobial isolates observed in the current study based on C-sources utilization may have ecological significance for their exploitation in future inoculation programmes.…”

Section: Resultssupporting

confidence: 52%

“…The genetic diversity of slow-and fast-growing rhizobia nodulating soybean was reported in many parts of the world. Recently Appunu et al (2008) studied the genetic diversity of soybean-nodulating Bradyrhizobium isolates from India and found that the diversity is wider than expected based on previous studies in various geographic areas and on the current taxonomy of soybean rhizobia.…”

mentioning

confidence: 99%

“…Nevertheless, the significance of rhizobia forming root nodules and growth enhancement in soybean was widely studied by many workers in the recent past (Saeki et al 2006, Sharma 2006. However, selection of niche-based new elite strains adapted to local environmental conditions and to newly bred plant lines stays a need of an hour (Appunu et al 2008).…”

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confidence: 99%

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Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of Central India

Sharma¹,

Srivastava²,

Sharma³

2010

PLANT SOIL ENVIRON

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Soybean cultivation in many zones of India shows occurrence of native rhizobia besides other exotically adapted strains. In the current study, 22 rhizobial isolates (recovered from 12 different soybean growing sites) and 8 reference strains were selected for biochemical and metabolic characterization. Of 22 isolates, 18 were recovered as fast growing isolates while the rest were slow growing based on bromothymol blue (BTB) test. Unlike earlier belief that rhizobia have no ability to grow on glucose peptone agar medium, in this study, some isolates and some reference strains grew well on this medium. Similarly, when all the isolates were subjected to ketolactose test, some of the isolates were found to show growth on the medium. In contrast, based on C-utilization pattern (15 carbohydrates) a remarkable metabolic diversity was observed among the rhizobial isolates recovered in the study. The clustering and matching analysis showed that most of isolates were matching with slow growing reference strains, a few were with fast growing reference strains and some were found to be unique and hence not matching with any of reference strains. Such analysis suggests the occurrence of metabolically distinct types of rhizobia besides commonly known types (B. japonicum, B. elkanii and S. fredii) of soybean rhizobia and further validation is suggested through 16SrRNA gene sequencing technique.

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Section: Resultssupporting

confidence: 52%

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confidence: 99%

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Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of Central India

Sharma¹,

Srivastava²,

Sharma³

2010

PLANT SOIL ENVIRON

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“…Previously there were reports on the isolation and characterization of B. yuanmingense strains that nodulated mungbean and soybean 9,22 and B. yuanmingense strain that did not nodulate soybean but nodulated legume species of M STB30 STB38 STB269 STB54 STB67 STB235 STB286 STB96 STB250 STB310 STB169 STB264 M bp 12,000 5,000 the genus Lespedeza 23 . In this study, two B. yuanmingense strains STB169 and STB264 were isolated.…”

Section: Discussionmentioning

confidence: 99%

“…Presently, there are two recognized strains of fast-growing soybean rhizobia, namely Sinorhizobium fredii and S. xinjiangense 4,5 . In addition, there are 4 recognized strains of slow-growing soybean rhizobia, i.e., Bradyrhizobium elkanii, B. japonicum, B. liaoningense, and the recently-discovered B. yuanmingense biovar that nodulates soybeans [6][7][8][9] . At present, there is not much information on polyphasic taxonomy of soybean rhizobia in Thailand [10][11][12][13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

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Maruekarajtinpleng¹,

Homhaul²,

Chansa-ngavej³

2012

ScienceAsia

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Soybean rhizobia are Gram negative bacteria that fix nitrogen in root nodules of soybeans. Selection of soybean rhizobia from present and previous soybean cultivation areas is one way to obtain efficient strains for inoculant production. At present, information on the diversity of soybean rhizobia in Thailand is scarce. The experiments aimed to isolate and characterize soybean rhizobium strains in soils from 16 subdistricts in Phitsanulok province, Thailand. Host trapping method was used to isolate bacteria from root nodules of 5 soybean cultivars grown in soils from the 16 subdistricts. Identical RAPD-PCR fingerprints revealed the 202 slow-growing isolates consisted of 121 strains. Authentication tests using 5 soybean cultivars showed all the 121 slow-growing strains had nodulated soybean roots. Four types of colony morphology on yeast extract mannitol containing congo red agar plates were obtained for all the isolated strains. Bromothymol blue (BTB) reactions on BTB agar plates revealed two types of slow-growing soybean rhizobia. Type 1 secreted alkali products after 5-day incubation, and acidic products upon prolonged incubation for another 5 days. Type 2 secreted only alkali products after 5-and 10-day incubation. Results from nucleotide sequences of 16S rDNA revealed 7, 6, and 2 strains of Bradyrhizobium elkanii, B. japonicum, and B. yuanmingense, respectively. The B. elkanii and B. japonicum strains were found to be natural variants with different RAPD-PCR fingerprints. This study is the first report on the findings of B. yuanmingense as well as natural variants of slow-growing soybean rhizobia in Thailand.

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Microevolution of symbiotic Bradyrhizobium populations associated with soybeans in east North America

Tang

Bromfield

Rodrigue

et al. 2012

Ecology and Evolution

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Microevolution and origins of Bradyrhizobium populations associated with soybeans at two field sites (A and B, 280 km apart in Canada) with contrasting histories of inoculation was investigated using probabilistic analyses of six core (housekeeping) gene sequences. These analyses supported division of 220 isolates in five lineages corresponding either to B. japonicum groups 1 and 1a or to one of three novel lineages within the genus Bradyrhizobium. None of the isolates from site A and about 20% from site B (the only site with a recent inoculation history) were attributed to inoculation sources. The data suggest that most isolates were of indigenous origin based on sequence analysis of 148 isolates of soybean-nodulating bacteria from native legumes (Amphicarpaea bracteata and Desmodium canadense). Isolates from D. canadense clustered with B. japonicum group 1, whereas those from A. bracteata were placed in two novel lineages encountered at soybean field sites. One of these novel lineages predominated at soybean sites and exhibited a significant clonal expansion likely reflecting selection by the plant host. Homologous recombination events detected in the 35 sequence types from soybean sites had an effect on genetic diversification that was approximately equal to mutation. Interlineage transfer of core genes was infrequent and mostly attributable to gyrB that had a history of frequent recombination. Symbiotic gene sequences (nodC and nifH) of isolates from soybean sites and native legumes clustered in two lineages corresponding to B. japonicum and B. elkani with the inheritance of these genes appearing predominantly by vertical transmission. The data suggest that soybean-nodulating bacteria associated with native legumes represent a novel source of ecologically adapted bacteria for soybean inoculation.

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Genetic Diversity of Native Bradyrhizobia Isolated from Soybeans ( Glycine max L.) in Different Agricultural-Ecological-Climatic Regions of India

Cited by 87 publications

References 28 publications

Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of Central India

Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of Central India

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Microevolution of symbiotic Bradyrhizobium populations associated with soybeans in east North America

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