Genetic diversity of oral Fusobacterium nucleatum isolated from patients with different clinical conditions

Abstract

SUMMARYThe genetic diversity of 23 oral Fusobacterium nucleatum isolated from 15 periodontal patients, eight from seven healthy subjects, nine from nine AIDS patients and two from two Cebus apella monkeys were analyzed. EcoRI restricted the bacterial DNA and 28 ribotypes grouped from A to J groups were obtained. Isolates formed 24 ribotypes which were contained into A, B, C, D, E and F groups, and three reference strains and two clinical isolates of A. actinomycetemcomitans, and E. coli CDC formed four different ribotypes into the G, H, I and J groups. Moreover, from nine F. nucleatum from AIDS patients, six were ribotyped as group C and three as group D. By using ribotyping we distinguished F. nucleatum recovered from different sources. It is possible that isolates from AIDS patients may contain some phenotypic or genotypic factor did not observed in this study.

Etiological Analysis of Initial Colonization of Periodontal Pathogens in Oral Cavity

“…Those pathogens found in Brazilian patients with periodontal diseases include A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia, C. rectus, Eikenella corrodens, Treponema denticola, Streptococcus spp., and some others (3,4,6,7,8,9,24,26,32,33,34). In particular, these previous studies focused on the prevalence of periodontal pathogens in diseased subjects representing four different stages of clinical treatment, as follows: (i) before periodontal treatment, (ii) after nonsurgical mechanical therapy (scaling and root planing), (iii) after antibiotic therapy, and (iv) after surgical treatment.…”

Fusobacterium nucleatum un comensal puente con otros microorganismos patógenos de la periodontitis

“…Se pueden hacer pruebas bioquímicas de fermentación de carbohidratos (glucosa, galactosa, lactosa, sacarosa y fructosa) para su identificación en su condición de cepa (26) . Pruebas moleculares pueden realizarse para la identificación de F. nucleatum, como la PCR (reacción en cadena de polimerasa), la cual tiene una gran especificidad y sensibilidad para detectar este microorganismo de muestras de biofilm subgingival (27,28) .…”

Fusobacterium nucleatum un comensal puente con otros microorganismos patógenos de la periodontitis

“…Se pueden hacer pruebas bioquímicas de fermentación de carbohidratos (glucosa, galactosa, lactosa, sacarosa y fructosa) para su identificación en su condición de cepa (26) . Pruebas moleculares pueden realizarse para la identificación de F. nucleatum, como la PCR (reacción en cadena de polimerasa), la cual tiene una gran especificidad y sensibilidad para detectar este microorganismo de muestras de biofilm subgingival (27,28) .…”