Genetic diversity of porcine sapoviruses

Jeong, Chaehwan; Park, Sang-Ik; Park, Sung-Hee; Kim, Ha-Hyun; Park, Su‐Jin; Jeong, Jae-Ho; Choy, Hyon E.; Saif, Linda J.; Kim, Sang‐Ki; Kang, Mun-Il; Hyun, Bang-Hun; Cho, Kyoung-Oh

doi:10.1016/j.vetmic.2007.02.008

Cited by 33 publications

(22 citation statements)

References 31 publications

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“…The primers used and the selection of samples tested by the age and the state of health of the pigs also differed in those studies, making direct comparisons of the results of the different studies difficult, but it can be concluded that sapovirus infection in pigs is common. However, our data confirm that pigs are infected with sapoviruses early in life, a finding that is in line with the findings of studies performed in Venezuela, Brazil, South Korea, and the United States (1,9,16,26). As also described in the papers from Venezuela, Brazil, and Belgium (17), we found sapoviruses in nearly equal numbers in pigs with and without diarrhea as well.…”

Section: Discussionsupporting

confidence: 92%

Incidence, Diversity, and Molecular Epidemiology of Sapoviruses in Swine across Europe

et al. 2010

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Porcine sapovirus is an enteric calicivirus in domestic pigs that belongs to the family Caliciviridae. Some porcine sapoviruses are genetically related to human caliciviruses, which has raised public health concerns over animal reservoirs and the potential cross-species transmission of sapoviruses. We report on the incidence, genetic diversity, and molecular epidemiology of sapoviruses detected in domestic pigs in a comprehensive study conducted in six European countries (Denmark, Finland, Hungary, Italy, Slovenia, and Spain) between 2004 and 2007. A total of 1,050 swine fecal samples from 88 pig farms were collected and tested by reverse transcription-PCR for sapoviruses, and positive findings were confirmed by sequencing. Sapoviruses were detected in 80 (7.6%) samples collected on 39 (44.3%) farms and in every country. The highest prevalence was seen among piglets aged 2 to 8 weeks, and there was no significant difference in the proportion of sapoviruspositive findings for healthy animals and animals with diarrhea in Spain and Denmark (the only countries where both healthy animals and animals with diarrhea were tested). On the basis of the sequence of the RNA polymerase region, highly heterogeneous populations of viruses representing six different genogroups (genogroups III, VI, VII, and VIII, including potential new genogroups IX and X) were identified, with a predominance of genogroup GIII (50.6%). Genogroup VIII, found in five of the six countries, had the highest degree of homology (up to 66% at the amino acid level) to human sapovirus strains. Sapoviruses are commonly circulating and endemic agents in swine herds throughout Europe. Highly heterogeneous and potential new genogroups of sapoviruses were found in pigs; however, no "human-like" sapoviruses were detected.

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Section: Discussionsupporting

confidence: 92%

Incidence, Diversity, and Molecular Epidemiology of Sapoviruses in Swine across Europe

et al. 2010

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“…Several previous studies have reported the prevalence rates and the genetic features of porcine NoVs and SaVs in Korea [15,[23][24][25]. The results of phylogenetic analyses of porcine NoVs in Korea revealed that all strains belonged to the typical porcine genotypes, such as GII-21 and GII-23.…”

Section: Introductionmentioning

confidence: 89%

“…Porcine NoVs were detected only in asymptomatic finisher pigs, but porcine SaVs could be identified in both diarrheic and asymptomatic pigs [15,[23][24][25]. Several previous studies have reported the prevalence rates and the genetic features of porcine NoVs and SaVs in Korea [15,[23][24][25].…”

Section: Introductionmentioning

confidence: 92%

Identification of genetic diversity of porcine Norovirus and Sapovirus in Korea

Song

Nam

et al. 2011

Virus Genes

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It is well known that Norovirus (NoV) and Sapovirus (SaV) identified in humans and pigs have heterogeneous genome sequences. In this study, a total of three strains of NoV and 37 strains of SaV were detected in 567 porcine fecal samples by RT-PCR, corresponding detection rates of 0.5 and 6.5%, respectively. Phylogenetic analyses were conducted using amino acid sequences of the partial RNA-dependent RNA polymerase (RdRp) and complete capsid proteins of both viruses to determine their genogroups. Analysis with the RdRp sequences indicated that all three NoV strains HW41, DG32, and DO35 detected in this study were classified into genogroup II (GII). A further analysis with the complete capsid sequence demonstrated that the DO35 strain belonged to subgenotype b in GII-21 (GII-21b) along with the SW918 strain. A total of 26 strains out of 27 strains that were selected from the 37 porcine SaVs were classified into genogroup III when they were analyzed with the RdRp sequences. The remaining strain (DO19) was not clustered with any of the previously classified SaV strains, thereby suggesting the advent of a new genogroup virus. Additional analyses with the amino acid sequence of the capsid and the nucleotide sequence of the RdRp and capsid junction region supported the notion that the DO19 strain belonged to a novel genogroup of SaV. To the best of our knowledge, this is the first report to describe a novel porcine SaV belonging to an unknown genogroup in Korea.

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“…SaVs are also classified into GI to GV, which are further subdivided into a number of clusters and genotypes, according to the genetic variation of their capsid or RdRp genes (8,16,36,41). GI, GII, GIV, and GV have been detected in humans, while GIII has been detected in swine.…”

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confidence: 99%

Frequent Detection of Noroviruses and Sapoviruses in Swine and High Genetic Diversity of Porcine Sapovirus in Japan during Fiscal Year 2008

Nakamura

Saga²,

Iwai

et al. 2010

J Clin Microbiol

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A molecular biological survey on porcine norovirus (NoV) and sapovirus (SaV) was conducted in Toyama Prefecture, Japan, during fiscal year 2008. Both NoV and SaV were detected from swine fecal samples throughout the surveillance period, indicating that these viruses were circulating in this region. NoV strains detected in this study belonged to three genotypes that are known as typical swine NoVs. Although human NoVs were occasionally detected, it was unclear whether they replicated in pigs. As for SaV, genogroup VII (GVII) and other divergent genogroups were identified in addition to the dominant genogroup, GIII, which is the prototypic porcine SaV. In addition, 3 strains genetically related to human SaV were detected. Two of these 3 strains were closely related to human SaV GV. Our study showed that genetic diversification of porcine SaV is currently progressing in the swine population.

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Genetic diversity of porcine sapoviruses

Cited by 33 publications

References 31 publications

Incidence, Diversity, and Molecular Epidemiology of Sapoviruses in Swine across Europe

Incidence, Diversity, and Molecular Epidemiology of Sapoviruses in Swine across Europe

Identification of genetic diversity of porcine Norovirus and Sapovirus in Korea

Frequent Detection of Noroviruses and Sapoviruses in Swine and High Genetic Diversity of Porcine Sapovirus in Japan during Fiscal Year 2008

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