Genetic Diversity of Potato Based on Random Amplified Polymorphic DNA and Simple Sequence Repeat Marker

Hadi, Sapto Nugroho; Nurchasanah, Siti

doi:10.18196/pt.2020.114.54-62

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…The characteristics of a plant can be demonstrated using an analysis of genetic diversity through morphological and molecular approaches. Both approaches use markers to demonstrate the characteristics of a plant [25]. Morphological characterization is the starting point in the description and classification of germplasm.…”

Section: B Vegetative Characters Of Kepel In the Palace Of Yogyakartamentioning

confidence: 99%

Diversity of Morphologically Vegetative Characters of Kepel (Stelechocarpus burahol): Study in Yogyakarta Palace Region, Indonesia

Handayani¹,

Parjanto²,

Cahyani³

et al. 2021

International Journal on Advanced Science, Engineering and Information Technology

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Kepel or Stelechocarpus burahol is a plant member of the Annonaceae family that is very useful as herbal medicines and natural deodorant for the Javanese palace's monarch. Kepel is an endemic Javanese plant that is currently very rare and endangered, so preservation and conservation are essential. The study aims to characterize vegetative morphology and determine the clustering of Kepel in Yogyakarta Palace Region as the first step for Kepel conservation. The study was carried out by a survey method using purposive sampling in 14 regions in Yogyakarta Palace Region. The plant used as an accession was healthy, grows normally, and has produced fruits. The variable observation of the canopy, stem, and leaf refers to the Annonaceae family's description based on Bioversity International. Data analysis is performed using cluster analysis based on the coefficient of similarity. The results showed that 46 kepels in Yogyakarta Palace Region had a high diversity based on the analysis of similarities in 16 variables with a similarity coefficient of 0.5-0.91. Analysis of cluster described that there are two clusters with a coefficient similarity of 0.5. Cluster A has 40 accessions (87%); cluster B has six accessions (13%). Leaf shape, leaf size, and stem color have an essential role in clustering the Kepel in Yogyakarta Palace Region. Cluster A relatively has a smaller leaf than the leaf of cluster B. The leaf blade shape of cluster A is elliptic to lanceolate, and the stem colors are beige-green and grey. Cluster B has an elliptical to ovate leaf blade shape, and the stem color is light brown, brown, and reddish-brown. This research data can enrich Kepel germplasm information, which is very useful as basic data for the conservation, development, and breeding of Kepel plants.

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Section: B Vegetative Characters Of Kepel In the Palace Of Yogyakartamentioning

confidence: 99%

Diversity of Morphologically Vegetative Characters of Kepel (Stelechocarpus burahol): Study in Yogyakarta Palace Region, Indonesia

Handayani¹,

Parjanto²,

Cahyani³

et al. 2021

International Journal on Advanced Science, Engineering and Information Technology

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“…SSR markers can efficiently facilitate the establishment of genetic linkages due to their high polymorphism level, codominant inheritance, high allele diversity level, randomly wide distribution in the genome, multi-allele, and experimentally reproducible and transferable among related species [9], [10]. Some molecular markers have been applied to the genetic diversity study on potatoes in Indonesia [5], [11]- [15], but only few reported with SSR markers. Therefore, this present study was aimed to quantify the genetic diversity among 29 elite potato varieties in Indonesia and 4 clones that originated from the United States using the 12 SSR markers and to identify the potential genotypes for potato breeding programs.…”

Section: Introductionmentioning

confidence: 99%

Diversity of 33 Genotypes of Potato Revealed by Simple Sequence Repeats Markers

Terryana,

Prihaningsih,

Nugroho

et al. 2022

JOAAT

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Genetic diversity analysis is essential for developing newly high-yielding potato varieties along with other important traits. Simple Sequence Repeats (SSR) markers can be robust tools to assess the genetic diversity of this plant species because they are abundant in the genome, codominant, and more accurate than morphological markers. This study was carried out to quantify the genetic divergence of 29 elite potato varieties in Indonesia and 4 clones that originated from the United States using the 12 SSR markers and identify the potential genotypes for potato breeding programs. A total of 136 gene alleles were detected from 12 SSR markers. The number of alleles per marker ranged from 2 to 22, with an average value of 12.8. All SSR markers showed Polymorphism Information Content (PIC) of 0.66-0.92, with an average of 0.80, and an average value of genetic diversity of 0.82, indicating their high suitability for potato diversity studies. Clustering and principal coordinate analysis classified 33 genotypes into three groups with a coefficient of similarity of 0.76, indicating their high genetic variability. All clones originating from the United States belonged to the same group and separated from the other genotypes. This study gives an overview of the genetic diversity of the Indonesian potato and provides an initial basis of selection for appropriate parents to assist breeders efficiently in developing newly potato varieties with desired agricultural traits in Indonesia.

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Genetic Diversity of Potato Based on Random Amplified Polymorphic DNA and Simple Sequence Repeat Marker

Abstract: The decline in the potato production also occurred in Central Java, which is one of the potato production centers in Indonesia. Data from the Indonesian Ministry of Agriculture ( 2017) stated that the number of

Cited by 2 publications

References 13 publications

Diversity of Morphologically Vegetative Characters of Kepel (Stelechocarpus burahol): Study in Yogyakarta Palace Region, Indonesia

Diversity of Morphologically Vegetative Characters of Kepel (Stelechocarpus burahol): Study in Yogyakarta Palace Region, Indonesia

Diversity of 33 Genotypes of Potato Revealed by Simple Sequence Repeats Markers

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