Genetic diversity of stilbene metabolism in Vitis sylvestris

Duan, Dong; Halter, David; Baltenweck, Raymonde; Tisch, C.; Tröster, Viktoria; Kortekamp, A.; Hugueney, Philippe; Nick, Peter

doi:10.1093/jxb/erv137

Cited by 76 publications

(92 citation statements)

References 43 publications

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“…Viniferin is highly toxic and can be considered an important marker for grapevine resistance to downy mildew (Pezet et al, 2004), but very little information is available on the role of constitutive stilbenoids (Duan et al, 2015). …”

Section: Resultsmentioning

confidence: 99%

Grapevine Grafting: Scion Transcript Profiling and Defense-Related Metabolites Induced by Rootstocks

et al. 2017

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Rootstocks are among the main factors that influence grape development as well as fruit and wine composition. In this work, rootstock/scion interactions were studied using transcriptomic and metabolic approaches on leaves of the “Gaglioppo” variety, grafted onto 13 different rootstocks growing in the same vineyard. The whole leaf transcriptome of “Gaglioppo” grafted onto five selected rootstocks showed high variability in gene expression. In particular, significant modulation of transcripts linked to primary and secondary metabolism was observed. Interestingly, genes and metabolites involved in defense responses (e.g., stilbenes and defense genes) were strongly activated particularly in the GAG-41B combination, characterized in addition by the down-regulation of abscisic acid (ABA) metabolism. On the contrary, the leaves of “Gaglioppo” grafted onto 1103 Paulsen showed an opposite regulations of those transcripts and metabolites, together with the greater sensitivity to downy mildew in a preliminary in vitro assay. This study carried out an extensive transcriptomic analysis of rootstock effects on scion leaves, helping to unravel this complex interaction, and suggesting an interesting correlation among constitutive stilbenes, ABA compound, and disease susceptibility to a fungal pathogen.

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Section: Resultsmentioning

confidence: 99%

Grapevine Grafting: Scion Transcript Profiling and Defense-Related Metabolites Induced by Rootstocks

et al. 2017

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“…A1). In a previous study (Duan et al 2015), we identified considerable variation in stilbene inducibility among grapevine (Vitis) genotypes, and reported differences in their abundance and profiles in response to UV-C pulsing. The V. sylvestris genotypes Hoe29, Ke53 and Ke83 and the V. vinifera cultivar Pinot Blanc were found to develop elevated stilbene levels in response to UV light, with high stilbene accumulation, and were classified as high-stilbene types, with the most notable representatives being Hoe29 and Ke53.…”

Section: Introductionmentioning

confidence: 91%

“…sylvestris genotypes 'Hoe29', 'Ke53' and 'Ke83', and the V. vinifera cultivars 'Augster Weiss', 'Pinot Blanc', and 'Müller-Thurgau', are maintained as living specimens in the grapevine collection of the Botanical Garden of the Karlsruhe Institute of Technology, Germany (Olmo 1976, Ledesma-Krist et al 2014). The V. sylvestris genotypes Hoe29, Ke53 and Ke83 and the V. vinifera cultivar Pinot Blanc were classified as high-stilbene types, while two V. vinifera cultivars, Augster Weiss and Müller-Thurgau have been categorized as low-stilbene types in response to UV light (Duan et al 2015). For the analysis in this study, entire leaves of a similar age (based on node size and position), were harvested from greenhouse-grown plants cultivated at a temperature of 22°C and 18°C (day and night, respectively) and a photoperiod of 14 h light and 10 h dark.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Frozen leaves were ground in liquid nitrogen using a pestle and mortar. A 300 mg fresh weight of powdered leaf tissue was mixed with 1 ml of 100% methanol and homogenized for 10 min on a platform vortexer in order to maximize uniform sampling and completely extract the stilbenes (Duan et al 2015). All the experiments were performed under a green safelight (λmax = 550 nm).…”

Section: Analysis and Quantification Of Trans-resveratrol And Viniferinmentioning

confidence: 99%

“…All the experiments were performed under a green safelight (λmax = 550 nm). The stilbenes were analyzed by liquid chromatography-mass spectrometry (LC-MS) at the Institut National de Recherche Agriculturel (INRA, Université de Strasbourg, Colmar, France), using the method described by Duan et al (2015). The instruments were operated using the XCalibur software and data were processed using the the XCMS software (Smith et al 2006).…”

Section: Analysis and Quantification Of Trans-resveratrol And Viniferinmentioning

confidence: 99%

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Changes in the chlorophyll content of grape leaves could provide a physiological index for responses and adaptation to UV‐C radiation

Luo

Jiang

et al. 2019

Nordic Journal of Botany

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Stilbenes are a group of phytoalexins that play an important role in grapevine (Vitis) basal immunity and can be induced by biotic and abiotic stresses. The levels of chlorophylls, the main pigments in plant cells, can also indicate the tolerance of plants to various stresses. Here, the response of different grapevine genotypes to UV‐C radiation treatment was tested and the abundance of chlorophyll in the Hoe29 and Ke53 genotypes was observed to increase significantly within 6 h of UV‐C treatment. Conversely, chlorophyll levels decreased markedly in the Augster Weiss and Müller–Thurgau genotypes. Furthermore, stilbene abundance increased substantially in the Hoe29, Ke53, Ke83 and Pinot Blanc genotypes, but increased only slightly in Augster Weiss and Müller–Thurgau. The expression of resveratrol synthase, which encodes a key enzyme in the stilbene synthesis pathway, increased in Hoe29, Ke53, Ke83 and Pinot Blanc following UV‐C treatment, in a manner consistent with stilbene accumulation. In addition, we observed that reactive oxygen species (ROS) provide a key trigger in physiological responses and changes in secondary metabolite contents. In summary, the results from this study support a link between ROS, chlorophyll levels and genetic diversity for stilbene abundance in different grape genotypes, providing insights into mechanisms for plant physiological and biochemical responses and adaptations to stress.

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MYB Genes Involved in Domestication and Crop Improvement

Albert

Allan

2021

Annual Plant Reviews Online

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Domestication of the world's crop species has been achieved through improvements in yield, and product qualities, with or without the guidance of scientific knowledge. These selection pressures are applied by breeders (and before them, farmers) both intentionally and unintentionally to improve crop features, which have often been grouped into ‘production traits’ and ‘quality traits’. New approaches to breeding that incorporate genome sequencing, mapping, and identification of genes underlying traits have revealed new variants in the family of transcription factors (TFs) called MYBs as driving important features in both these categories of domestication traits. In this article, we review the progress in understanding the role of MYB TFs in crop performance, as well as reasons to predict further gains in yield and quality centred on the MYB TF family.

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Genetic diversity of stilbene metabolism in Vitis sylvestris

Abstract: HighlightWe show that the ancestor of cultivated grapevine harbours genetic factors that increase the inducibility of stilbenes correlated with increased resistance to the important pathogen grapevine downy mildew.

Cited by 76 publications

References 43 publications

Grapevine Grafting: Scion Transcript Profiling and Defense-Related Metabolites Induced by Rootstocks

Grapevine Grafting: Scion Transcript Profiling and Defense-Related Metabolites Induced by Rootstocks

Changes in the chlorophyll content of grape leaves could provide a physiological index for responses and adaptation to UV‐C radiation

MYB Genes Involved in Domestication and Crop Improvement

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