Genetic diversity of Taenia saginata (Cestoda: Cyclophyllidea) from Lao People’s Democratic Republic and northeastern Thailand based on mitochondrial DNA

Sanpool, Oranuch; Rodpai, Rutchanee; Intapan, Pewpan M.; Sadaow, Lakkhana; Thanchomnang, Tongjit; Laymanivong, Sakhone; Maleewong, Wanchai; Yamasaki, Hiroshi

doi:10.1186/s13071-017-2079-7

Cited by 12 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differently from the other studies in the central, north and northeastern areas of Lao PDR, where cases of T . asiatica were not found [ 12 , 14 , 15 ], in the east part of Savannakhet province ( Fig 1 ) we could detect the presence of 3 species of human Taenia species in Sepon district that border Vietnam. The prevalence of Taenia eggs in KK was not high as presented by Okello et al [ 14 ] which detected percentages as high as 46%.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Taenia solium, Taenia saginata, Taenia asiatica, their hybrids and other helminthic infections occurring in a neglected tropical diseases' highly endemic area in Lao PDR

Sato

Yanagida

et al. 2018

PLoS Negl Trop Dis

View full text Add to dashboard Cite

Most part of Southeast Asia is considered endemic for human-infecting Taenia tapeworms; Taenia solium, T. saginata, and T. asiatica. However, until now there was no report of the occurrence of human cases of T. asiatica in Lao PDR. This study, conducted in Savannakhet Province, Lao PDR, microscopically examined a total of 470 fecal samples by Kato Katz method and found 86% of people harboring at least one helminth. Hookworms were detected in 56% of the samples besides Opisthorchis like eggs (42%), Trichuris trichiura (27%), Ascaris spp. (14%), and Taenia spp. (4%) eggs. Serology for cysticercosis showed 6.8% positives with results varying from 3% to 14.3% in Ethnic School students and Kalouk Kao village respectively. Species-specific PCR targeting mitochondrial DNA (mtDNA) of 28 tapeworms, recovered from 16 patients, revealed T. solium (n = 2), T. saginata (n = 21), and T. asiatica (n = 5). Two patients were confirmed to be coinfected with T. saginata and T. asiatica, indicating the endemicity of the 3 human Taenia in Lao PDR. However, nucleotide sequencing of a nuclear DNA gene, DNA polymerase delta (pold) revealed that all the tapeworms identified as T. asiatica using mtDNA had T. saginata type allele at pold locus, demonstrating that they are not “pure T. asiatica” but the hybrid descendants between the two species, confirming the wide distribution of hybrids of T. saginata/ T. asiatica in Southeast Asia. The high prevalence of several helminthic NTDs in east Savannakhet area even with conventional control measures indicates the importance to establish wide and multifaceted health programs to sustainably improve the quality of life of the populations living in these communities.

show abstract

Section: Discussionmentioning

confidence: 94%

“…Furthermore, in a recent study, 15 haplotypes of T . saginata were obtained from 30 isolates from Khammouane Province, central Lao PDR [ 15 ]. An extensive study on the prevalence of taeniasis in Lao PDR with whole country coverage reported the presence of mainly T .…”

Section: Introductionmentioning

confidence: 99%

Taenia solium, Taenia saginata, Taenia asiatica, their hybrids and other helminthic infections occurring in a neglected tropical diseases' highly endemic area in Lao PDR

Sato

Yanagida

et al. 2018

PLoS Negl Trop Dis

View full text Add to dashboard Cite

show abstract

“…In the last decade, several phylogenetic analyses were performed and all demosntrated genetic diversity in F. hepatica [4] , L. tropica [5] , T. saginata [6] , E. multilocularis [7] , G. lamblia [8] ; Blastocystis spp. [9] ; E. granulosus [10] .…”

Section: Applicationsmentioning

confidence: 99%

What is and why do we have to know the phylogenetic tree?

Abaza

2020

Parasitologists United Journal

View full text Add to dashboard Cite

What we have to know? The term phylogenetic is derived from the two ancient Greek words (phûlon) and (génesis). The first means "race or lineage", and the latter means "origin or source". Parasitologists have to know that phylogeny of a group of species, called a clade, is based upon similarities and/or differences in their biological, morphological, and genetic characteristics. To comprehend a phylogenetic tree, one must keep in mind that it is composed of internal branches that connect two nodes or it is formed of just one node with one external branch. The node is a branching diverging point, while the crown node, i.e. the tree root, represents the last common ancestor of the clade of interest. The clade is a monophyletic group of creatures that can be separated from the rest of the tree, including its root, by cutting a single branch. In other words, monophyletic species are all the descendants of a particular ancestor, included in one clade. The root is the earliest node in the phylogenetic tree that represents the last common ancestor of all tree tips or leaves. The latter are also known as the entities (e.g., texa, genes) whose relationships are depicted using the tree diagram. Therefore, texa are a named group of organisms that are represented by tree tips or leaves (Figure 1 A-C). A cladogram represents the branching pattern of texa and provides all the information relevant to determine the degree of relatedness between the known group of organisms. This means that the cladogram indicates that the more closely related organisms are those which share more recent common ancestors. It also provides time distance between evolutionary lineage splitting which is the division of an ancestral lineage into two or more descendant lineages. It is worth mentioning that the history of lineage splitting, depicted by a rooted tree including the list of all its clades, is termed the tree's topology. During the last two decades, several algorithmic programs were developed for phylogenetic analyses for estimation of the lineage divergence time between species.

show abstract

“…Taenia saginata, Taenia solium, and Taenia asiatica are responsible for human taeniasis. Among them, T. saginata and T. solium are distributed globally, while T. asiatica is mostly distributed in Asian countries [1][2][3][4][5]. The symptomatology of infection is broad, but the most frequent and distinctive symptoms are the discharge of proglottids, abdominal pain, weight loss, nausea, and fever [6].…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification and Appraisal of the Genetic Variation of Taenia saginata in Central Regions of Vietnam

Thi

Azzena

Scarpa

et al. 2022

Life

View full text Add to dashboard Cite

Taenia saginata is a globally distributed tapeworm responsible for human taeniasis due to the ingestion of raw or undercooked beef. T. saginata is present in several Asian countries, including China, Thailand, Lao PDR, Cambodia, and Vietnam, but little is known about its genetic variation. Studying the tapeworm’s phylogeographic patterns is crucial to better understanding their association with the geographic distribution of taeniasis/cysticercosis in human populations. In the present study, 38 specimens of this putative species were collected in central regions of Vietnam and analysed using the mitochondrial gene Cytochrome c Oxidase subunit I (COI) as a molecular marker to assess the correct species identification and investigate the level of genetic variation at different geographic scales. Phylogenetic and phylogeographic analyses were carried out on a dataset that included COI sequences from Vietnamese specimens and from all conspecifics available in GenBank to date. The results showed that the collected Vietnamese specimens belonged to the species T. saginata. In Southeast Asia, signs of a possible founder effect were discovered, with the most common haplotypes frequent and present in many countries, except Lao PDR, which shares its most common haplotype only with individuals from Thailand. Remarkably, a unique taxonomic entity was found worldwide, even though the available COI sequences of T. saginata belonging to non-Asiatic countries are, at present, limited. Therefore, future studies including more COI sequences from a higher number of countries and the use of a combined molecular approach with multiple genetic markers would be useful to provide deeper insight into the global genetic variation of this species.

show abstract

Genetic diversity of Taenia saginata (Cestoda: Cyclophyllidea) from Lao People’s Democratic Republic and northeastern Thailand based on mitochondrial DNA

Cited by 12 publications

References 30 publications

Taenia solium, Taenia saginata, Taenia asiatica, their hybrids and other helminthic infections occurring in a neglected tropical diseases' highly endemic area in Lao PDR

Taenia solium, Taenia saginata, Taenia asiatica, their hybrids and other helminthic infections occurring in a neglected tropical diseases' highly endemic area in Lao PDR

What is and why do we have to know the phylogenetic tree?

Molecular Identification and Appraisal of the Genetic Variation of Taenia saginata in Central Regions of Vietnam

Contact Info

Product

Resources

About