Genetic diversity of the black gram [&lt;i&gt;Vigna mungo&lt;/i&gt; (L.) Hepper] gene pool as revealed by SSR markers

Kaewwongwal, Anochar; Kongjaimun, Alisa; Somta, Prakit; Chankaew, Sompong; Yimram, T.; Srinives, Peerasak

doi:10.1270/jsbbs.65.127

Cited by 54 publications

(47 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, none of the QTLs for seed dormancy were common among these four Vigna species. In fact, we expected that black gram shares some common QTLs for pod indehiscence and seed dormancy with mungbean because the two species both originated and are domesticated in India and show very similar morphological characteristics and are cultivated and used in the same fashion ( Tomooka et al, 2002 ; Kaewwongwal et al, 2015 ). However, the results indicated that different mechanisms control seed dormancy in these five Vigna species.…”

Section: Discussionmentioning

confidence: 99%

QTL Mapping for Agronomic and Adaptive Traits Confirmed Pleiotropic Effect of mog Gene in Black Gram [Vigna mungo (L.) Hepper]

et al. 2020

Self Cite

View full text Add to dashboard Cite

Organ size and architecture of plants are important traits affecting crop yield and agronomic practices. An induced mutant, multiple-organ gigantism (MOG), of black gram ( Vigna mungo ) has been obtained, which shows gigantic leaves, fruit, seed, and architecture (plant height) but lower number of pods per plant. These traits are a pleiotropic effect of a single recessive gene, mog . In this study, we investigated variation of 16 agronomic and adaptive traits in a recombinant inbred line (RIL) population derived from a cross between the MOG mutant ( V. mungo var. mungo ) and wild black gram ( V. mungo var. silvestris ) accession TC2210 and identified quantitative trait loci (QTLs) controlling those traits to gain a better understanding of the effect of the mog gene on breeding. The results showed that most of the traits (100-seed weight, leaf size, and plant height) showed moderate narrow-sense heritability ( h 2 ) (45–65%), while pod size and seed length (SDL) showed high h 2 (>75%) and pod dehiscence (shattering), and seed width (SDW) and days to flowering showed low h 2 (<35%). The QTLs for the traits were mapped onto a high-density linkage map developed for the RIL population. Inclusive composite interval mapping identified 42 QTLs in total for the 16 traits with number of QTLs per trait ranging from one to six. Major QTLs for the MOG phenotypes were clustered on linkage group (LG) 6, confirming the pleiotropic effect of the mog gene. Effect of the mog gene/QTL for the MOG phenotypic variations was not high, ranging from about 15% in plant height to 40% in leaf size. For 100-seed weight, which is the most interesting trait, the mog gene/QTL contributed about 30% of the total trait variation and showed an additive effect of only 0.51 g, which is only about 1.5-fold higher than that of the other five QTLs detected for this trait. These results indicated that mog gene expression is highly affected by environment and the effect of the gene toward organ size and plant height is not extraordinarily high. Implications of the findings of this study and exploiting of the MOG mutant in breeding were also discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

QTL Mapping for Agronomic and Adaptive Traits Confirmed Pleiotropic Effect of mog Gene in Black Gram [Vigna mungo (L.) Hepper]

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Because they are stable, fast, multi‐allelic, and require less DNA, SSR molecular markers have been extensively used for genetic diversity, quantitative trait loci, and association analyses in black gram [ Vigna mungo (L.) Hepper], maize ( Zea mays L.), rice ( Oryza sativa L.), and tobacco (Obara et al, 2010; Yang et al, 2013; Zhang et al, 2014; Kaewwongwal et al, 2015). Dai et al (2015) analyzed the genetic diversity of 33 tobacco resistant backbone parents based on 103 SSR markers and obtained a PIC value of 0.343.…”

Section: Discussionmentioning

confidence: 99%

Analysis on Solanesol Content and Genetic Diversity of Chinese Flue‐Cured Tobacco (Nicotiana tabacum L.)

Xiang

Yao²,

Liu

et al. 2017

Crop Science

View full text Add to dashboard Cite

Solanesol is an important pharmaceutical intermediate that also affects the quality of tobacco (Nicotiana tabacum L.) leaves. To explore novel applications of tobacco leaves and to broaden the genetic basis of flue‐cured tobacco breeding varieties, the solanesol content of 168 Chinese flue‐cured tobacco germplasm resources, planted at four geographical regions of China in 2014 and 2015, was determined using ultra‐performance liquid chromatography. Solanesol content and genetic diversity were analyzed using additive main effects and multiplicative interaction model (AMMI) and simple sequence repeat (SSR) markers. The results indicated that solanesol content of flue‐cured tobacco ranged from 0.70 to 4.13% and the mean content of samples from the northern regions (2.60%) were higher than that from the southern regions (1.67%) of China. Genotypes, environments, and their interactions all had significant impacts on the solanesol content of tobacco leaves. Additionally, high‐solanesol varieties Dashuba2106, Changboyan, and Heiyeyan and low‐solanesol cultivars Zhongyan90, CF90NF, Zhongyan102 had the highest hereditary stability. These 168 accessions were divided into introduced (Y), breeding (X), and local (D) groups according to origins. Using 69 SSR primer pairs selected from 1196 primer combinations, we scored 271 alleles, of which 229 (84.50%) were polymorphic. Shannon's index, Nei's index, and polymorphism information content value were 0.8006, 0.4613, and 0.4014, respectively, suggesting a wide genetic basis of these 168 germplasm resources. Among the three groups, the D group showed the richest genetic diversity, while the Y group exhibited the lowest genetic diversity. The Y and X groups had the highest genetic similarity (0.9657), followed by X group versus D group (0.9462) and Y group versus D group (0.9019). Findings in this study should have great importance for future exploitation and utilization of solanesol from tobacco leaves.

show abstract

“…Vigna mungo (L.) Hepper or black gram is a diploid plant with 2 n = 2 x = 22 chromosomes. It belongs to the family Leguminosae, subfamily Papilionoideae, clade Millettioid [ 1 ], and is a tropical legume crop species that is cultivated in Asia, Africa, and America [ 2 ]. Black gram is an economically important Vigna species, which provides high-protein food [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

The Genome and Transcriptome Analysis of the Vigna mungo Chloroplast

Nawae

Yundaeng

Naktang

et al. 2020

Plants

Self Cite

View full text Add to dashboard Cite

Vigna mungo is cultivated in approximately 5 million hectares worldwide. The chloroplast genome of this species has not been previously reported. In this study, we sequenced the genome and transcriptome of the V. mungo chloroplast. We identified many positively selected genes in the photosynthetic pathway (e.g., rbcL, ndhF, and atpF) and RNA polymerase genes (e.g., rpoC2) from the comparison of the chloroplast genome of V. mungo, temperate legume species, and tropical legume species. Our transcriptome data from PacBio isoform sequencing showed that the 51-kb DNA inversion could affect the transcriptional regulation of accD polycistronic. Using Illumina deep RNA sequencing, we found RNA editing of clpP in the leaf, shoot, flower, fruit, and root tissues of V. mungo. We also found three G-to-A RNA editing events that change guanine to adenine in the transcripts transcribed from the adenine-rich regions of the ycf4 gene. The edited guanine bases were found particularly in the chloroplast genome of the Vigna species. These G-to-A RNA editing events were likely to provide a mechanism for correcting DNA base mutations. The V. mungo chloroplast genome sequence and the analysis results obtained in this study can apply to phylogenetic studies and chloroplast genome engineering.

show abstract

Genetic diversity of the black gram [<i>Vigna mungo</i> (L.) Hepper] gene pool as revealed by SSR markers

Cited by 54 publications

References 33 publications

QTL Mapping for Agronomic and Adaptive Traits Confirmed Pleiotropic Effect of mog Gene in Black Gram [Vigna mungo (L.) Hepper]

QTL Mapping for Agronomic and Adaptive Traits Confirmed Pleiotropic Effect of mog Gene in Black Gram [Vigna mungo (L.) Hepper]

Analysis on Solanesol Content and Genetic Diversity of Chinese Flue‐Cured Tobacco (Nicotiana tabacum L.)

The Genome and Transcriptome Analysis of the Vigna mungo Chloroplast

Contact Info

Product

Resources

About