Genetic diversity of the natural strains of Streptococcus thermophilus

Ботина, С. Г.; Piksasova, O. V.; Tsygankov, Yu. D.; Sukhodolets, V. V.

doi:10.1134/s102279540705002x

Cited by 2 publications

(3 citation statements)

References 16 publications

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“…The molecular typing of all high folate producing isolates along with two reference strains (NCDC 177, National Culture Collection Centre, National Dairy Research Institute, Karnal, Haryana, India;and MTCC 1938, (Microbial Type Culture Collection, Institute of Microbial Technology, Chandigarh, India)) wass done by RAPD-PCR using two primer variants: M13, 5 0 -gagggtggcggttct-3 0 (Botina, Piksasova, Tsygankov, & Sukhodolets, 2007) and AP4, 5 0 -tcacgctgca-3 0 (Andrighetto et al, 2001).…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

Probiotic properties of folate producing Streptococcus thermophilus strains

Iyer

Tomar

Kapila

et al. 2010

Food Research International

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Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

Probiotic properties of folate producing Streptococcus thermophilus strains

Iyer

Tomar

Kapila

et al. 2010

Food Research International

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“…This modified PCR program used for RAPD PCR was found to be more efficient than that described by Botina et al (2007) and Andrighetto et al (2001Andrighetto et al ( , 2002. On the basis of RAPD, no clear correlation can be deduced between RAPD clusters and folate production by isolates.…”

Section: Resultsmentioning

confidence: 92%

“…The interspecific differences of ten high folate producing isolates was characterized by random amplification of polymorphic DNA (RAPD)-PCR with two type strains S. thermophilus NCDC 177 and MTCC 1938 as standard cultures and comparative analysis of the PCR fingerprinting profiles similarity using two RAPD primers M13, 5′-GAGGGTGGCGGTTCT-3′ (Botina et al 2007) and AP4, 5′-TCACGCTGCA-3′ (Andrighetto et al 2001). The RAPD-PCR was performed with minor modification in the annealing temperatures for both the primers.…”

Section: Screening For Folate Productionmentioning

confidence: 99%

Bioprospecting of strains of Streptococcus thermophilus from Indian fermented milk products for folate production

Iyer

Tomar

Mohanty

et al. 2011

Dairy Science & Technol.

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This study deals with the bio-prospecting of folate producing strains of Streptococcus thermophilus isolated from milk and different fermented milk products of Indian origin. From a total of 500 randomly selected colonies isolated from 209 different samples, 117 isolates were identified as S. thermophilus by classical biochemical and molecular characterization. Frequency of incidence of S. thermophilus in the different samples of milk and milk products was variable with the highest in the dahi followed by yogurt and lassi and a very low incidence in case of milk and cheese. On screening for folate using a microbiological assay with a trienzyme extraction, about 15% of strains was found to produce folate in the range of 40-50 μg·L −1 , 35% in the range of 20-30 μg·L −1 , and the remainder in the range of 4-16 μg·L −1 . Comparative analysis of the random amplification of polymorphic DNA PCR fingerprint profiles was used to characterize interspecific diversity of the ten highest folate producers. The LacZ gene of two of the highest folate producing isolates were sequenced and submitted to GenBank under following accession numbers of FJ161697 and FJ161698.

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Genetic diversity of the natural strains of Streptococcus thermophilus

Cited by 2 publications

References 16 publications

Probiotic properties of folate producing Streptococcus thermophilus strains

Probiotic properties of folate producing Streptococcus thermophilus strains

Bioprospecting of strains of Streptococcus thermophilus from Indian fermented milk products for folate production

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