Genetic diversity of Tunisian figs (Ficus carica L.) as revealed by nuclear microsatellites

Abstract

The present study portrays the achievement of the genetic polymorphism surveying and the establishment of an ecotypes identification key on the basis of simple sequence repeats data. Seventy‐two Tunisian fig ecotypes in situ and ex situ conserved were analyzed using six microsatellite loci. A total of 58 alleles and 124 genotypes were revealed and permitted to evidence high degree of genetic diversity mainly explained at the intra group level. Cluster analysis based on genetic distances proved that a typical continuous genetic diversity characterizes the local germplasm. In addition, the microsatellite multilocus genotyping has permitted to unambiguously distinguish 70 well‐defined ecotypes (resolving power of 97.22%). Data are discussed in relation with the reliability of the used markers to check the conformity of the plant material and to rationally manage the conservation of this crop.

Assessment of genetic diversity of Tunisian orange, Citrus sinensis (L.) Osbeck using microsatellite (SSR) markers

“…In the present, it was possible to differentiate the two trees of Washington navel cultivar, WN1 and WN2 by SSR specific alleles. These results confirm the effectiveness of the SSR markers in genotyping and the identification of cultivars, as was done previously on the date palm (Zehdi et al, 2012), apricot (Krichen et al, 2006), fig (Saddoud et al, 2007;Chatti et al, 2010), olive (Taamalli et al, 2008), and almond (Gouta et al, 2012) by SSR markers.…”

Development of an SSR-based identification key for Tunisian local almonds

“…With only five primers, it was possible to discriminate among all landraces studied, identifying 103 alleles and 155 different genotypes. In fig (Ficus carica L.), it was not possible to discriminate among all 72 Tunisian local ecotypes with six SSR primers, but the identification key revealed 58 alleles and 124 genotypes, for a resolving power of 97.2 % (Saddoud et al, 2007). An identification key for 26 Tunisian olives (Olea europea L.) was successful in discriminating among all local cultivars using three of ten SSR markers (Taamalli et al, 2008).…”

Genetic Diversity of Fig (Ficus carica L.) Based on Morphological Characters and Two-Way Hierarchical Cluster Analysis

“…In the fig accessions, 26 of 38 fruit characters could explain 47.3% of the total variation. The most important discriminators of fig fruits were the fruit weight, fruit length, fruit diameter, fruit skin and flesh color (Saddoud et al 2008;Podgornik et al 2010), fruit shape (Giraldoet al 2010;Podgornik et al 2010), firmness of the fruit skin (Saddoud et al 2008;Podgornik et al 2010), fruit skin cracks (Saddoud et al 2008), production type, skin firmness (Giraldo et al 2010), fruit neck length (Podgornik et al 2010), abscission of the stalk from the twig (Podgornik et al 2010), stalk diameter, neck diameter, ostiole diameter, ostiole opening, and flesh thickness.…”

“…Only 11 out of 26 plant traits could successfully distinguish different accessions. The number and shape of lobes (Saddoud et al 2008), tree growth habit, size of the tree, degree of branching, number of lobes per leaf (Giraldo et al 2010), leaf length, leaf width, leaf area, density of hairs/spicules on the leaf 's upper surface, and petiole thickness (Podgornik et al 2010) were the traits used for the discrimination of fig accessions.…”