Genetic diversity studies of Kherigarh cattle based on microsatellite markers

Abstract

We report a genetic diversity study of Kherigarh cattle, a utility draught-purpose breed of India, currently declining at a startling rate, by use of microsatellite markers recommended by the Food and Agriculture Organization. Microsatellite genotypes were derived, and allelic and genotypic frequencies, heterozygosities and gene diversity were estimated. A total of 131 alleles were distinguished by the 21 microsatellite markers used. All the microsatellites were highly polymorphic, with mean (+/- s.e.) allelic number of 6.24 +/- 1.7, ranging 4-10 per locus. The observed heterozygosity in the population ranged between 0.261 and 0.809, with mean (+/- s.e.) of 0.574 +/- 0.131, indicating considerable genetic variation in this population. Genetic bottleneck hypotheses were also explored. Our data suggest that the Kherigarh breed has not experienced a genetic bottleneck in the recent past.

Characterization of rare migratory cattle and evaluation of its phylogeny using short-tandem-repeat-based markers

“…However, considerable high heterozygosity values indicated the existence of substantial genetic variability in the studied population. Similar high values for observed and expected heterozygosity have been observed for Kherigarh (0.71) cattle (Pandey, Rekha, Singh, et al 2006) However, many workers reported comparatively lower mean expected heterozygosity, 0.67 in Tharparkar cattle and Hariana cattle (Rehman & Khan 2009); 0.59 in Deoni cattle (Mukesh et al 2004) and 0.68 in Kherigarh cattle (Pandey, Rekha, Yatender, et al 2006). The mean observed heterozygosity values, though lower than the expected values, exhibited failure of significant differences suggesting random mating in Belahi cattle.…”

“…Most of the loci showed a similar trend in terms of allele numbers with the earlier characterized cattle breeds. The observed number of alleles in Belahi cattle was higher than observed in other Indian breeds (Mukesh et al 2004;Pandey, Rekha, Singh, et al 2006;Pandey, Rekha, Yatender, et al 2006), namely Hariana (6.5), Sahiwal (5.2), Deoni (5.9), Kenkatha (5.95) and Kherigarh (3.77) cattle. Our findings had shown moderately higher number of alleles; this could be due to the presence of higher heterogeneity present in Belahi population.…”

“…Our results were similar to those reported by Sharma et al (2013) in Bachaur, Shahbadi, Purnea and Siri cattle from Northeast of India. Slightly higher inbreeding estimates of 0.14 and 0.18 were reported in Tharparkar and Kherigarh cattle, respectively (Pandey, Rekha, Singh, et al 2006;Sodhi et al 2006). However, comparatively higher inbreeding estimates were reported in Sahiwal (0.32), Kenkatha (0.21) and Hariana (0.25) cattle (Mukesh et al 2004;Pandey, Rekha, Yatender, et al 2006;Rehman & Khan 2009).…”

“…They are neutral markers and have high variability, widespread distribution throughout the genome and have shown a co-dominant inheritance. Microsatellites are best suited to evaluate genetic diversity (Metta et al 2004;Li et al 2005;Yoon et al 2005;Sodhi et al 2005Sodhi et al , 2006Pandey, Rekha, Singh, et al 2006, Pandey, Rekha, Yatender, et al 2006Sharma et al 2013) and relationships among cattle populations from Africa, Europe and Asia (Moazami-Goudarzi et al 1997;Machugh et al 1998;Martín-Burriel et al 1999;Mommens et al 1999;Beja-Pereira et al 2003;Jordana et al 2003). Using a set of bovine-specific microsatellite markers, Mukesh et al (2004) studied the genetic diversity (both within and between) and relationship among the three important Indian cattle breeds, namely Sahiwal, Hariana and Deoni.…”

Comparison of microsatellite and blood group diversity among different genotypes of cattle

“…The main value was 0.009 and it indicates 0.9% gene fixation within the breeds analysed. This value is lower than that obtained by Pandey et al (2006) in the Indian cattle population (0.188). F IT value varied from -0.018 (TGLA227) to 0.371 (ETH10).…”