A study was conducted to evaluate the impact of probiotic, prebiotic and synbiotic as feed additives on faecal counts of coliform and E. coli along with cell mediated immune response in crossbred calves. For this purpose fifteen days old, 24 crossbred calves were divided into 4 groups of 6 calves each. The calves were assigned to different dietary treatments viz T 0 (control group) -basal diet, T 1 (probiotic group) -basal diet + Lactobacillus acidophilus @ 1 g/calf/day (2 x 10 10 cfu/ g), T 2 (prebiotic group) -basal diet + Mannan oligosaccharide (MOS) @ 4 g per calf/day and T 3 (synbiotic group) basal diet +Lactobacillus acidophilus @ 0.5 gm (2 x 10 10 cfu/ g) + 2 g MOS per calf/day respectively for a period of 90 days. Faecal samples for coliform and E. coli count were taken at 15 days interval. Blood samples were taken at 15 days interval for lymphocyte count. Blood samples were taken at 45 th and 90 th -day for lymphocyte stimulation test (LST) in EDTA coated vials. Skin fold test was done on 90 th day of experimental study. The results of study showed that there was a significant reduction in faecal shedding of coliform and E. coli in T 1 , T 2 and T 3 groups indicating lesser proliferation of pathogenic bacteria in the gut. The treatment groups T 2 and T 3 showed a significant increase in lymphocyte count whereas all the treated groups had significantly higher values of the lymphocytic index on 45 th and 90 th day post supplementation indicating improved cell mediated immunity. Increased skin fold thickness was observed post 48 hours of phytohaemagglutinin injection in T 2 and T 3 groups. It may, therefore, be concluded that supplementation of prebiotic and synbiotic is advantageous in attaining gut conditioning and enhanced immunity which may be helpful in preventing calf scours in crossbred calves.

A study was conducted on 24 fifteen day old crossbred calves that were divided into 4 groups of 6 calves each. The calves were fed different dietary treatments viz T 0 (control group) -basal diet, T 1 (probiotic group) -basal diet + Lactobacillus acidophilus @ 1 g/calf/day (2 x 10 10 cfu/ g), T 2 (prebiotic group) -basal diet + Mannan oligosaccharide (MOS) @ 4 g per calf/day and T 3 (synbiotic group) basal diet + Lactobacillus acidophilus @ 0.5 gm (2 x 10 10 cfu/ g) + 2 g MOS per calf/day respectively for a period of 90 days. Blood samples were taken at monthly interval and serum was separated to estimate different biochemical parameters. The results of study indicated that probiotic (Lactobacillus acidophilus), prebiotic (MOS) and synbiotic supplementation were effective in reduction of serum cholesterol and triglycerides at 60 th and 90 th day. Serum creatinine, glucose, urea and bilirubin were unaffected by dietary supplementation of probiotic, prebiotic (MOS) and synbiotic. There was no effect of T 1, T 2 and T 3 supplementation on Serum enzymes, Aspartate aminotransferase (AST) and Alanine Amino Transferase (ALT).

The present study was conducted to evaluate the impact of probiotic, prebiotic

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